Effect of nutrient limitation and two-stage continuous fermentor design on productivities during “Clostridium ragsdalei” syngas fermentation

Kundiyana, Dimple K.; Huhnke, Raymond L.; Wilkins, Mark R.

doi:10.1016/j.biortech.2011.03.020

Cited by 54 publications

(25 citation statements)

References 30 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…"C. ragsdalei" or strain "P11" was isolated from duck pond sediment by researchers from The University of Oklahoma and Oklahoma State University and is described in a patent [154]. "C. ragsdalei" has been explored for the production of ethanol from syngas [155][156][157], with growth temperatures of 32 °C-37 °C [157] and a batch fermentation reported ethanol concentration of 1.99 g L −1 [158]. In a 100 L stirred tank reactor (STR) an ethanol concentration of 25.26 g L −1 was achieved over a fermentation duration of 59 days [159].…”

Section: Ethanol Productionmentioning

confidence: 99%

Commercial Biomass Syngas Fermentation

2012

View full text Add to dashboard Cite

Abstract:The use of gas fermentation for the production of low carbon biofuels such as ethanol or butanol from lignocellulosic biomass is an area currently undergoing intensive research and development, with the first commercial units expected to commence operation in the near future. In this process, biomass is first converted into carbon monoxide (CO) and hydrogen (H 2 )-rich synthesis gas (syngas) via gasification, and subsequently fermented to hydrocarbons by acetogenic bacteria. Several studies have been performed over the last few years to optimise both biomass gasification and syngas fermentation with significant progress being reported in both areas. While challenges associated with the scale-up and operation of this novel process remain, this strategy offers numerous advantages compared with established fermentation and purely thermochemical approaches to biofuel production in terms of feedstock flexibility and production cost. In recent times, metabolic engineering and synthetic biology techniques have been applied to gas fermenting organisms, paving the way for gases to be used as the feedstock for the commercial production of increasingly energy dense fuels and more valuable chemicals.

show abstract

Section: Ethanol Productionmentioning

confidence: 99%

Commercial Biomass Syngas Fermentation

2012

View full text Add to dashboard Cite

show abstract

“…The baffled CSTR (continuously-stirred tank reactor) fermenter equipped with gas dispersion impellers can be operated in semi-batch mode with batch liquid and continuous gas feed, with fed-batch liquid, or with both continuous gas and liquid feed. Two-stage CSTR fermenters have been operated with the first CSTR configured to promote growth of the acetogenic culture with acid production and the second CSTR operated at low pH under nutrient limitation and low gas conversion to achieve high ethanol concentration [100]. Column fermenters that show promise include a bubble column with a ceramic monolith to support biofilm [84], a trickle bed with biofilm [87,101,102] and a biofilm supported on a hollow fiber membrane for gas dispersion [86,87].…”

Section: Reactor Designmentioning

confidence: 99%

Syngas Fermentation: A Microbial Conversion Process of Gaseous Substrates to Various Products

2017

View full text Add to dashboard Cite

Biomass and other carbonaceous materials can be gasified to produce syngas with high concentrations of CO and H 2 . Feedstock materials include wood, dedicated energy crops, grain wastes, manufacturing or municipal wastes, natural gas, petroleum and chemical wastes, lignin, coal and tires. Syngas fermentation converts CO and H 2 to alcohols and organic acids and uses concepts applicable in fermentation of gas phase substrates. The growth of chemoautotrophic microbes produces a wide range of chemicals from the enzyme platform of native organisms. In this review paper, the Wood-Ljungdahl biochemical pathway used by chemoautotrophs is described including balanced reactions, reaction sites physically located within the cell and cell mechanisms for energy conservation that govern production. Important concepts discussed include gas solubility, mass transfer, thermodynamics of enzyme-catalyzed reactions, electrochemistry and cellular electron carriers and fermentation kinetics. Potential applications of these concepts include acid and alcohol production, hydrogen generation and conversion of methane to liquids or hydrogen.

show abstract

“…In another study with C. ragsdalei, limiting calcium pantothenate, vitamin B12 and cobalt chloride in two-stage continuous bioreactor resulted in 15 g ethanol/g cell compared to 2.5 g ethanol/g cell in a single-stage bioreactor (Kundiyana et al, 2011a). Standard YE medium was replaced with defined minimal medium, cotton seed extract (CSE) and corn steep liquor (CSL) to reduce medium cost and improve ethanol production (Gao et al, 2013;Kundiyana et al, 2010;Maddipati et al, 2011;Phillips et al, 2014).…”

Section: Medium Componentsmentioning

confidence: 99%