Performance evaluation of the new fully automated urine particle analyser UF-5000 compared to the reference method of the Fuchs-Rosenthal chamber

Previtali, Giulia; Ravasio, Rudi; Seghezzi, Michela; Buoro, Sabrina; Alessio, Maria Grazia

doi:10.1016/j.cca.2017.07.028

Cited by 56 publications

(51 citation statements)

References 18 publications

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“…Nevertheless, with the development of new products and technologies, a new, fully automated, third generation urine flow cytometer UF5000 following the UF100 and UF1000i is now available. A study suggests that the UF5000 has an increased performance compared with UF1000i on the whole, except for WBC [19]. Otherwise, no study of BACT was done.…”

Section: Discussionmentioning

confidence: 99%

The Diagnostic Performance of Urine Flow Cytometer UF1000i for Urinary Tract Infections

Sun¹,

Zuo²,

Liu³

et al. 2018

Clin. Lab.

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Background: Urinary tract infections (UTIs) are one of the most common infectious diseases in clinic. Urine flow cytometry is receiving more and more attention due to its rapid forecast of UTIs. Methods: The Urine Flow Cytometer UF1000i has a series of software programs to quantify bacteria (BACT) and white blood cells (WBC), and describe the scatter diagram of bacteria. The UTIs were predicted based on the cutoff values with the Receiver Operating Characteristic (ROC) curves of BACT and WBC counts. To evaluate the diagnostic performance of UF1000i for UTIs, the sensitivity and specificity of 889 urine samples were determined in comparison to the results of urine culture. Meanwhile the bacterial morphology indication of the UF1000i was evaluated in order to help doctors choose antibiotics. The angle of the scatter cloud with the x-axis was used to classify the infected bacteria as bacilli (< 30°) or cocci (≥ 30°). Results: The best cutoff value of BACT counts for predicting UTIs was 119 per µL, and the sensitivity and specificity were 95.5% and 88.7%, respectively. While the best cutoff value of WBC counts was 81.5 per µL, and the sensitivity and specificity were 77.6% and 76.7%, respectively. In addition, the best cutoff values for females were 583 BACT per µL and 137.5 WBC per µL. They were much higher than for males (118 BACT per µL and 91 WBC per µL). The coincidence of the bacterial morphology information between the UF1000i software indication and the bacterial actual morphology identified by urine culture was 83% (bacilli) and 68% (cocci), respectively. Conclusions: Data demonstrated that the performance of BACT counts for UTIs is superior to WBC counts. In addition, the bacterial morphology could preliminarily be predicated by the scatter diagram. Since the urine flow cytometer UF1000i can provide the data of both BACT counts and the scatter diagram, the urine flow cytometry was regarded as a suitable method for screening UTIs. Moreover, it would be better to take gender into consideration when setting the best cutoff value for diagnosis of UTIs in clinic.

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Section: Discussionmentioning

confidence: 99%

The Diagnostic Performance of Urine Flow Cytometer UF1000i for Urinary Tract Infections

Sun¹,

Zuo²,

Liu³

et al. 2018

Clin. Lab.

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show abstract

“…The cutoff for both RBCs and WBCs was set at 25 cells/µL. The UF‐5000 showed very good agreement when compared to phase‐contrast microscopy using a Fuchs‐Rosenthal chamber . The Behring Nephelometer II analyzer (Siemens, Marburg, Germany) was used for assessment of urinary albumin concentration (cutoff 20 mg/L).…”

Section: Methodsmentioning

confidence: 99%

“…The UF-5000 showed very good agreement when compared to phase-contrast microscopy using a Fuchs-Rosenthal chamber. 12 The Behring Nephelometer II analyzer (Siemens, Marburg, Germany) was used for assessment of urinary albumin concentration (cutoff 20 mg/L). The immunonephelometric assay was carried out using commercially available Siemens antibodies and the WHO/College of American Pathologists certified reference material 470.…”

Section: Reference Methodsmentioning

confidence: 99%

Semiquantitative, fully automated urine test strip analysis

Oyaert

Delanghe

2019

Clinical Laboratory Analysis

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ObjectivesUrinalysis is one of the most frequently ordered diagnostic laboratory tests. In order to reduce workload and costs, rapid screening tests such as urine test strip analyses are applied. The aim of this study was to evaluate the analytical performance of the UC‐3500 as well as the diagnostic performance in comparison with reference methods.Design and methodsWe measured within‐run and between‐run imprecision based on quantitative reflectance values. 347 prospectively included urine specimens were investigated for the presence of glucose, protein, albumin, leukocyte esterase, and hemoglobin peroxidase activity, and ordinal scale results were compared to an automated urine particle analyzer (UF‐5000, Sysmex, Kobe, Japan) and wet chemistry (Roche Cobas 8000, Mannheim, Germany).ResultsWithin‐run and between‐run imprecision results based on reflectance data for both the 9 and 11 parameter test strips ranged from 0.07% to 1.36% for the low‐level control and from 0.37% to 6.13% for the high‐level control, depending on the parameter. Regarding diagnostic performance, the sensitivity/specificity for glucose, protein, albumin, leukocyte esterase, and hemoglobin peroxidase was 100/60%, 94.2/88.2%, 81.8/89.2%, 81.7/92.8%, and 85.1/88.6%, respectively; the negative predictive value was 100%, 83.3%, 89.1%, 94.6%, and 96.1%. The Spearman correlation coefficients of the UC‐3500 vs reference methods ranged from 0.915 to 0.967, depending on the parameter.ConclusionThis fully automated urine test strip analyzer overall shows a satisfying performance and can reliably screen out negative urine samples in order to focus on further characterization of positive samples in the following steps of the workflow.

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“…Sysmex UF-5000 is a third-generation urine sediment analyzer that uses flow cytometry [17]. Each cell is stained with fluorescent dyes and passed through the flow lamina.…”

Section: Introductionmentioning

confidence: 99%

Performance Evaluation of Body Fluid Cellular Analysis Using the Beckman Coulter UniCel DxH 800, Sysmex XN-350, and UF-5000 Automated Cellular Analyzers

Cho

Lee

et al. 2020

Ann Lab Med

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Background: Automated cellular analyzers are expected to improve the analytical performance in body fluid (BF) analysis. We evaluated the analytical performance of three automated cellular analyzers and established optimum reflex analysis guidelines.Methods: A total of 542 BF samples (88 cerebrospinal fluid [CSF] samples and 454 non-CSF samples) were examined using manual counting and three automated cellular analyzers: UniCel DxH 800 (Beckman Coulter), XN-350 (Sysmex), and UF-5000 (Sysmex). Additionally, 2,779 BF analysis results were retrospectively reviewed. For malignant cell analysis, the receiver operating characteristic (ROC) curve was used, and the detection of high fluorescence-BF cells (HF-BFs) using the XN-350 analyzer was compared with cytology results.Results: All three analyzers showed good agreement for total nucleated cell (TNC) and red blood cell (RBC) counts, except for the RBC count in CSF samples using the UniCel DxH 800. However, variable degrees of differences were observed during differential cell counting. For malignant cell analysis, the area under the curve was 0.63 for the XN-350 analyzer and 0.76 for manual counting. We established our own reflex analysis guidelines as follows: HF-BFs < 0.7/100 white blood cells (WBCs) is the criterion for quick scans with 100 × magnification microscopic examination as a rule-out cut-off, while HF-BFs > 83.4/100 WBCs or eosinophils > 3.8% are the criteria for mandatory double check confirmation with 1,000 × magnification examination. Conclusions:The three automated analyzers showed good analytical performances. Application of reflex analysis guidelines is recommended for eosinophils and HF-BFs, and manual confirmation is warranted.

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Performance evaluation of the new fully automated urine particle analyser UF-5000 compared to the reference method of the Fuchs-Rosenthal chamber

Abstract: UF-5000 is an analyser of great interest to detect urine particle related to pathological process of kidney and urinary tract.

Cited by 56 publications

References 18 publications

The Diagnostic Performance of Urine Flow Cytometer UF1000i for Urinary Tract Infections

The Diagnostic Performance of Urine Flow Cytometer UF1000i for Urinary Tract Infections

Semiquantitative, fully automated urine test strip analysis

Performance Evaluation of Body Fluid Cellular Analysis Using the Beckman Coulter UniCel DxH 800, Sysmex XN-350, and UF-5000 Automated Cellular Analyzers

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