Performance evaluation of immunoassay for infectious diseases on the Alinity i system

Nam, Minjeong; Song, Da Young; Song, Sang Hoon; Roh, Eun Youn; Shin, Sue; Park, Kyoung Un; Song, Eun Young

doi:10.1002/jcla.23671

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…Anti-CMV IgG were detected using the Abbott Architect CMV IgG chemiluminescent microparticle immunoassay (CMIA) [ 21 , 22 ]. The assay uses a human CMV lysate (strain AD 169) and is performed on the automated platform Abbott Architect i2000SR (Abbott, Abbott Park, IL, United States).…”

Section: Methodsmentioning

confidence: 99%

CMV-IgG pre-allogeneic hematopoietic stem cell transplantation and the risk for CMV reactivation and mortality

Eberhardt

Jung

Knops

et al. 2023

Bone Marrow Transplant

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Cytomegalovirus (CMV) represents one of the most common infectious complications after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Currently, a common diagnostic test used to stratify the risk for CMV infection in allo-HSCT recipients is the qualitative CMV serology of donor and recipient. A positive serostatus of the recipient is the most important risk factor for CMV reactivation and associated with reduced overall survival post-transplantation (TX). Direct and indirect effects of CMV are involved in the poorer survival outcome. The present study investigated if the quantitative interpretation of anti-CMV IgG before allo-HSCT might serve as a novel parameter for the identification of patients at risk for CMV reactivation and worse outcome post-TX. For this purpose, a cohort of 440 allo-HSCT recipients over a period of 10 years was retrospectively analyzed. Our findings indicated that patients with high CMV IgG pre-allo-HSCT had a higher risk to develop CMV reactivation, including clinically relevant infections, and a worse prognosis 36 months post-allo-HSCT as compared to recipients with low CMV IgG values. In the letermovir (LMV) era, this group of patients might benefit from a closer CMV monitoring, and hence, earlier intervention if needed, especially after discontinuation of prophylaxis.

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Section: Methodsmentioning

confidence: 99%

CMV-IgG pre-allogeneic hematopoietic stem cell transplantation and the risk for CMV reactivation and mortality

Eberhardt

Jung

Knops

et al. 2023

Bone Marrow Transplant

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show abstract

“…The cutoff values for IgG or IgM detection recommended by the manufacturers were used to interpret the results (Table 1). For easier understanding, since several studies, including local ones (data not shown), have asserted an equivalence between AxSYM and Architect Toxo assays on the one hand (14,15) and between Architect and Alinity Toxo assays on the other hand (16); these assays were renamed Abbott Toxo IgG and IgM assays. All immunoassays reported the test results in IU/mL for IgG and index for IgM.…”

Section: Methodsmentioning

confidence: 99%

Diagnosis of Congenital Toxoplasmosis: Performance of Four IgG and IgM Automated Assays at Birth in a Tricentric Evaluation

et al. 2022

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For postnatal diagnosis of congenital toxoplasmosis (CT), the gold standard for the detection of anti- Toxoplasma IgM in newborns relies on the immunosorbent agglutination assay (ISAGA), which is manufactured from whole Toxoplasma parasites that become difficult to maintain. For IgG, only the Platelia assay provides a validated assay for cord blood according to the manufacturer, allowing its use in this context.

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“…Three investigators reviewed the results, and any samples that were positive for either IgG or IgM against T . gondii were retested and confirmed with the ARCHITECT i2000SR system (Abbott, Abbott Park, Illinois, U.S.A.) in accordance with the Clinical and Laboratory Standards Institute (CLSI) [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

“…A negative result was indicated by "IgG-IgM-", a positive IgG but negative IgM antibody result was indicated by "IgG+IgM-", a positive IgM but negative IgG antibody result was indicated by "IgG-IgM+", and a positive result for both IgG and IgM antibodies was indicated by "IgG+IgM+" (as shown in Fig 1). Three investigators reviewed the results, and any samples that were positive for either IgG or IgM against T. gondii were retested and confirmed with the ARCHITECT i2000SR system (Abbott, Abbott Park, Illinois, U.S.A.) in accordance with the Clinical and Laboratory Standards Institute (CLSI) [25].…”

Section: Rapid Diagnostic Test (Rdt) and Retest Of Positive Samplesmentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii infection among patients of a tertiary hospital in Guangzhou, Guangdong province, PR China

et al. 2023

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Purpose This study aimed to explore the prevalence of Toxoplasma gondii (T. gondii) among patients in Guangzhou city, South China, and to identify susceptible patient populations and analyze the causes of infection differences. Methods From May 2020 to May 2022, a total of 637 sera were collected from patients, and 205 sera were collected from health participants as health control. All sera were examined by colloidal gold kits to detect the positivity of antibodies against T. gondii. And the positivity of antibodies in sera was confirmed with ARCHITECT i2000SR system. Results The prevalence of T. gondii infection in patients was 7.06% (45/637), which was lower than the prevalence in health participants 4.88% (10/205). Among patients, 34 (5.34%) were positive only for IgG, 10 (1.57%) were only for IgM, and 1 (0.16%) was positive for both IgG and IgM. There was a significant difference in prevalence between male and female patients, but not among different age groups or diseases groups. The prevalence of T. gondii infection in diseases groups varied. The prevalence was relatively high in patients with the disorders of thyroid gland and the malignant neoplasms of digestive organs, which suggests that caution should be taken to avoid T. gondii infection in these patients. Surprisingly, the prevalence was quite low in diffuse Large B-cell Lymphoma (DLBC) patients. This may be due to the overexpression of TNF-α in tumor tissues of DLBC patients and the higher protein level of TNF-α in sera of DLBC patients. Conclusion This study provides a systematic exploration of the prevalence of T. gondii infection in patients in a tertiary hospital. Our data contributes to a better understanding of the epidemic investigation of T. gondii among patients in South China, which can help the prevention and treatment of the disease caused by T. gondii infection.

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Performance evaluation of immunoassay for infectious diseases on the Alinity i system

Cited by 7 publications

References 12 publications

CMV-IgG pre-allogeneic hematopoietic stem cell transplantation and the risk for CMV reactivation and mortality

CMV-IgG pre-allogeneic hematopoietic stem cell transplantation and the risk for CMV reactivation and mortality

Diagnosis of Congenital Toxoplasmosis: Performance of Four IgG and IgM Automated Assays at Birth in a Tricentric Evaluation

Seroprevalence of Toxoplasma gondii infection among patients of a tertiary hospital in Guangzhou, Guangdong province, PR China

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