Diagnosis of Congenital Toxoplasmosis: Performance of Four IgG and IgM Automated Assays at Birth in a Tricentric Evaluation

Avignon, Marine; Lévêque, Maude F.; Guemas, Emilie; Sasso, Milène; Albaba, Sahar; Lachaud, Laurence; Fillaux, Judith

doi:10.1128/jcm.00115-22

Cited by 5 publications

(3 citation statements)

References 20 publications

(24 reference statements)

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“…The lack of IgM detection could be at least partially attributed to maternal treatment (25,30). In the present study, we could detect IgM in 73.2% and 74.8% of infected infants with ISAGA and PLATELIA, respectively, which is in agreement with other studies evaluating postnatal diagnosis (18,30,31). As already shown in other studies, the rate of IgM detection was higher when maternal infection occurred during the third trimester of pregnancy, but was not linked to the IgM assay (25,32).…”

Section: Discussionsupporting

confidence: 92%

Could PLATELIA Toxo IgM be the new gold standard for the serological diagnosis of congenital toxoplasmosis: a French multicenter study

Arkhis,

Rouges,

Dahane

et al. 2024

J Clin Microbiol

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To assess the performance of PLATELIA Toxo IgM (Bio-Rad) and Toxo ISAGA (BioMérieux) to detect anti- Toxoplasma IgM in infants at risk of congenital toxoplasmosis, a retrospective multicenter study was conducted comparing serological results obtained in the framework of routine diagnosis work-up for congenital toxoplasmosis. All infants born to mothers infected with T. gondii during pregnancy from 2010 to 2020 with at least 6 months of serological follow-up were included ( n = 1,010). One thousand ten cases were included, of which 250 infants (24.75%) had congenital toxoplasmosis. A total of 1039 sera were included. The concordance between the two techniques was 96%, with kappa coefficient of 0.87, showing an almost perfect agreement between ISAGA and PLATELIA. Cumulative sensitivity and specificity were 73.2% and 99.5.% and 74.8% and 100% for ISAGA and PLATELIA, respectively. The mean time to detect IgM using ISAGA and PLATELIA tests was 6.9 ± 20.1 days and 5.6 ± 14.7 days, respectively not significant (ns). Finally, the sensitivity of ISAGA and PLATELIA to detect IgM antibodies in infected neonates at 5 days of life was 62% and 64%, respectively. Performances of PLATELIA Toxo IgM assay were comparable to the gold standard ISAGA. This enzyme-linked immunosorbent assay is suitable for routine serology for the diagnosis of congenital toxoplasmosis in newborns. IMPORTANCE This study will help clinical microbiologists to chose an alternative serological method for the neonatal diagnosis of congenital toxoplasmosis, once the gold standard technique ISAGA will be withdrawn next year.

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Section: Discussionsupporting

confidence: 92%

Could PLATELIA Toxo IgM be the new gold standard for the serological diagnosis of congenital toxoplasmosis: a French multicenter study

Arkhis,

Rouges,

Dahane

et al. 2024

J Clin Microbiol

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“…Platelia Toxo IgM Ò appears to be a good alternative to replace Toxo-ISAGA IgM Ò in neonates because of its very good sensitivity. This finding confirms previous studies [2,12]. Concerning adults, the accuracy of Liaison Toxo IgM Ò was greater than that of Toxo-ISAGA IgM Ò , but another study [12] reported that Liaison Toxo IgM Ò had lower sensitivity (61.7%) with a concordance of 51.7% with Toxo-ISAGA IgM Ò .…”

Section: Discussionsupporting

confidence: 88%

“…In a pregnant woman with negative IgG and positive IgM, the differential diagnosis between recent T. gondii infection and the presence of false IgM positivity is often difficult [6]. For Toxoplasma IgM detection, numerous automated immunoenzymatic or chemiluminescence tests [12] have been used, but a confirmatory test such as the IgM immunosorbent agglutination assay (ISAGA) is a help to determine whether the IgM are specific or not [2].…”

Section: Introductionmentioning

confidence: 99%

Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM?

Deleplancque,

Fricker-Hidalgo,

Pomares

et al. 2024

Parasite

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The ISAGA immunocapture test for the detection of anti-Toxoplasma immunoglobulin M is a manual technique known for its excellent sensitivity and specificity. The purpose of this retrospective, multicenter study was to compare the performances and agreement between ISAGA and other IgM detection techniques before cessation of ISAGA production. The analytic performance of the different tests was evaluated using 1,341 serum samples from adults with positive IgM and negative IgG to Toxoplasma gondii, and 1,206 sera from neonates born to mothers with seroconversion. The agreement between the tests was evaluated on 13,506 adult and 5,795 child serum samples. The sensitivity of Toxo-ISAGA IgM® (adults 98.7%, neonates 63.1%) was similar to that of Platelia Toxo IgM® (adults 94.4%, neonates 64.6%), and significantly higher than Liaison Toxo IgM® (adults 90.6%), Architect/Alinity Toxo IgM® (adults 95.7%, neonates 48.6%), and Vidas Toxo IgM® (adults 81.8%, neonates 17.5%). However, the specificities varied between 24.4% (Platelia Toxo IgM®) and 95.2% (Liaison Toxo IgM®) in adults and were >95% for all tests in neonates. An analysis of the kappa coefficients showed better agreement between ISAGA IgM® and the other tests in children (0.75–0.83%) than in adults (0.11–0.53%). We conclude that, in the absence of Toxo-ISAGA IgM®, the association of a very sensitive technique (Platelia Toxo IgM® or Architect/Alinity Toxo IgM®) and a very specific technique (Vidas Toxo IgM® or Liaison Toxo IgM®) is recommended for IgM detection in adult sera. For neonates, Platelia Toxo IgM® appeared to be the best alternative to replace Toxo-ISAGA IgM®.

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A retrospective study of air quality associated with teratogenic pathogen screening in women of reproductive age in southern China

Chen

Zheng

Huang

et al. 2023

Environ Sci Pollut Res

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Diagnosis of Congenital Toxoplasmosis: Performance of Four IgG and IgM Automated Assays at Birth in a Tricentric Evaluation

Cited by 5 publications

References 20 publications

Could PLATELIA Toxo IgM be the new gold standard for the serological diagnosis of congenital toxoplasmosis: a French multicenter study

Could PLATELIA Toxo IgM be the new gold standard for the serological diagnosis of congenital toxoplasmosis: a French multicenter study

Comparative performance of ISAGA IgM and ELISA assays for the diagnosis of maternal and congenital Toxoplasma infections: which technique could replace ISAGA IgM?

A retrospective study of air quality associated with teratogenic pathogen screening in women of reproductive age in southern China

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