Performance evaluation of fiber optic probes for tissue lifetime fluorescence spectroscopy

Papaioannou, Thanassis; Preyer, Norris W.; Fang, Qiyin; Kurt, Hamza; Carnohan, Michael; Ross, Russel; Brightwell, Adam; Cottone, Greg; Jones, Linda; Marcu, Laura

doi:10.1117/12.476141

Cited by 12 publications

(8 citation statements)

References 13 publications

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“…The application of fiber optic in spectroscopic analysis provides the ability of “ taking the spectrometer to the sample ” despite the conventional method of taking the sample to the spectrometer [ 57 ]. Moreover, the fiber optic probe allows flexible delivery and collection of scattered or reflected light even in hard to reach areas [ 58 ]. The ultimate goal of the design of water quality fiber sensors is to make them able to be implemented at site where the consideration of the velocity of flowing media and the variation of particles may be considered and good measurement can still be obtained.…”

Section: Discussionmentioning

confidence: 99%

Turbidimeter Design and Analysis: A Review on Optical Fiber Sensors for the Measurement of Water Turbidity

Omar¹,

MatJafri²

2009

Sensors

143

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Turbidimeters operate based on the optical phenomena that occur when incident light through water body is scattered by the existence of foreign particles which are suspended within it. This review paper elaborates on the standards and factors that may influence the measurement of turbidity. The discussion also focuses on the optical fiber sensor technologies that have been applied within the lab and field environment and have been implemented in the measurement of water turbidity and concentration of particles. This paper also discusses and compares results from three different turbidimeter designs that use various optical components. Mohd Zubir and Bashah and Daraigan have introduced a design which has simple configurations. Omar and MatJafri, on the other hand, have established a new turbidimeter design that makes use of optical fiber cable as the light transferring medium. The application of fiber optic cable to the turbidimeter will present a flexible measurement technique, allowing measurements to be made online. Scattered light measurement through optical fiber cable requires a highly sensitive detector to interpret the scattered light signal. This has made the optical fiber system have higher sensitivity in measuring turbidity compared to the other two simple turbidimeters presented in this paper. Fiber optic sensors provide the potential for increased sensitivity over large concentration ranges. However, many challenges must be examined to develop sensors that can collect reliable turbidity measurements in situ.

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Section: Discussionmentioning

confidence: 99%

Turbidimeter Design and Analysis: A Review on Optical Fiber Sensors for the Measurement of Water Turbidity

Omar¹,

MatJafri²

2009

Sensors

143

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“…The results of these studies are reported elsewhere. 37 In the current study, a custom-made bifurcated probe ͑nonsolarizing silica/silica step index fibers of 0.11 NA, CeramOptec, East Longmeadow, MA͒ was used. The probe consists of a central excitation fiber ͑600 m core diameter͒ surrounded distally by a collection ring of fourteen 200 m core diameter fibers.…”

Section: B Fiber-optic Probementioning

confidence: 99%

Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics

Fang

Papaioannou

et al. 2004

Review of Scientific Instruments

Self Cite

112

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Articles you may be interested inDesign and evaluation of a device for fast multispectral time-resolved fluorescence spectroscopy and imaging Rev. Sci. Instrum. 85, 034303 (2014); 10.1063/1.4869037 A multichannel time-domain scanning fluorescence mammograph: Performance assessment and first in vivo results Rev. Sci. Instrum. 82, 024302 (2011); 10.1063/1.3543820 Development of a dual-modal tissue diagnostic system combining time-resolved fluorescence spectroscopy and ultrasonic backscatter microscopy Rev. Sci. Instrum. 80, 065104 (2009); 10.1063/1.3142478 Design and development of a rapid acquisition laser-based fluorometer with simultaneous spectral and temporal resolution Rev. Sci. Instrum. 72, 3061 (2001); 10.1063/1.1379957Rapid acquisition, analysis, and display of fluorescence lifetime-resolved images for real-time applications Rev.We report the design and development of a compact optical fiber-based apparatus for in situ time-resolved laser-induced fluorescence spectroscopy ͑tr-LIFS͒ of biological systems. The apparatus is modular, optically robust, and compatible with the clinical environment. It incorporates a dual output imaging spectrograph, a gated multichannel plate photomultiplier ͑MCP-PMT͒, an intensified charge-coupled-device ͑ICCD͒ camera, and a fast digitizer. It can accommodate various types of light sources and optical fiber probes for selective excitation and remote light delivery/ collection as required by different applications. The apparatus allows direct recording of the entire fluorescence decay with high sensitivity ͑nM range fluorescein dye concentration with signal-to-noise ratio of 46͒ and with four decades dynamic range. It is capable of resolving a broad range of fluorescence lifetimes from hundreds of picoseconds ͑as low as 300 ps͒ using the MCP-PMT coupled to the digitizer to milliseconds using the ICCD. The data acquisition and analysis process is fully automated, enabling fast recording of fluorescence intensity decay across the entire emission spectrum ͑0.8 s per wavelength or ϳ40 s for a 200 nm wavelength range at 5 nm increments͒. The spectral and temporal responses of the apparatus were calibrated and its performance was validated using fluorescence lifetime standard dyes ͑Rhodamin B, 9-cyanoanthracene, and rose Bengal͒ and tissue endogenous fluorophores ͑elastin, collagen, nicotinamide adenine dinucleotide, and flavin adenine dinucleotide͒. Fluorescence decay lifetimes and emission spectra of all tested compounds measured with the current tr-LIFS apparatus were found in good agreement with the values reported in the literature. The design and performance of tr-LIFS apparatus have enabled in vivo studies of atherosclerotic plaques and brain tumors.

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“…Optical fiber-based fluorescence lifetime measurements are being researched by several groups [ 28 – 30 ]. Moreover, measurement of fluorescence decay lifetime, as opposed to steady state, has special advantages because it is relatively independent of light scattering, signal amplitude fluctuations, and fluorophore concentration [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates

Awad

Ramprasath

Mathivanan

et al. 2014

International Scholarly Research Notices

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Fluorescence spectroscopy was examined as a potential technique for identification and classification of bacterial pathogens. Colonies of Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae on agar plates were measured directly using a laboratory spectrofluorimeter coupled with optical fiber. Steady state fluorescence spectra were collected following excitation at 280 nm (tryptophan) and 380 nm (NADH). Results showed that fluorescence lifetime decays of tryptophan at 280 nm excitation from the four organisms were best described with triexponential fit and it reveals the existence of different protein conformation. The emission spectroscopy of the four bacteria at 380 nm excitation (NADH) provided better classification (100% of original grouped cases correctly classified and 98.1% of cross-validated grouped cases correctly classified) than that of 280 nm excitation (tryptophan). Our results demonstrated that optical fiber-based fluorescence identification and classification of bacteria is rapid, easy to perform, and of low cost compared to standard methods.

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Performance evaluation of fiber optic probes for tissue lifetime fluorescence spectroscopy

Cited by 12 publications

References 13 publications

Turbidimeter Design and Analysis: A Review on Optical Fiber Sensors for the Measurement of Water Turbidity

Turbidimeter Design and Analysis: A Review on Optical Fiber Sensors for the Measurement of Water Turbidity

Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics

Optical Fiber-Based Steady State and Fluorescence Lifetime Spectroscopy for Rapid Identification and Classification of Bacterial Pathogens Directly from Colonies on Agar Plates

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