Performance analysis of immobilized and co-immobilized enriched-mixed culture for hydrogen production

Damayanti, Astrilia; Sarto, Sarto; Sediawan, Wahyudi Budi; Syamsiah, Siti

doi:10.15282/jmes.12.1.2018.18.0312

Cited by 7 publications

(8 citation statements)

References 32 publications

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“…Immobilized material characteristics was similar to those in the previous experiment (Damayanti et al 2018). Two grams of alginate was dissolved in 100 mL distilled water.…”

Section: Immobilized and Co-immobilized Mixed Culturementioning

confidence: 85%

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Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System

Damayanti

Sarto

Sediawan

2020

Int. J. Renew. Energy Dev.

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Biohydrogen production via dark fermentation is a prospective renewable energy technology. In the process, reused of immobilized mixed culture is very important as their activities greatly influencehydrogen production. The aim of this work was to evaluate the reuse of alginate beads affecting the biohydrogen production for 45 days. This study in batch reactor were performed using glucose 10 M as substrate, alginate and activated carbon as immobilization matrix materials, chicken eggshell as buffer, and cow dung biodigester as mixed culture. Hydrogen and pH on fermentation product are investigated by gas chromatography (GC) technique and pH meter, respectively. The colony diameter on immobilized and co-immobilized mixed culture was observed using optical microscope and colony diameter was measured using Image-Pro Plus Software v4.5.0.29. The surface morphology of immobilization and co-immobilization beads were determined using scanning electron microscope (SEM). The results showed that the colonies growth observed using optical microscopy or SEM was apparent only in the immobilization of mixed culture. The average growth and diameter of colonies per day were 90 colonies and 0.025 mm, respectively. The weight of beads and pH during the 45-day fermentation process for bead immobilization of mixed culture were 1.32–1.95 g and 6.25–6.62, correspondingly, meanwhile, the co-immobilizations of the mixed culture were 1.735–2.21g and 6.25–6.61, respectively. In addition, the average hydrogen volume of glucose fermented using an eggshell buffer and reusing the immobilization and co-immobilization beads was 18.91 mL for 15 cycles.©2020. CBIORE-IJRED. All rights reserved

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“…Immobilized material characteristics was similar to those in the previous experiment (Damayanti et al 2018). Two grams of alginate was dissolved in 100 mL distilled water.…”

Section: Immobilized and Co-immobilized Mixed Culturementioning

confidence: 85%

“…The ratio of Alginate concentration to AC was 1:1. The preparation of immobilized and co-immobilized mixed culture was similar to that in the previous experiment (Damayanti et al 2018)…”

Section: Immobilized and Co-immobilized Mixed Culturementioning

confidence: 93%

Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System

Damayanti

Sarto

Sediawan

2020

Int. J. Renew. Energy Dev.

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“…The optimum ratio of GAC to alginate of 1:2 remarked the occupation of the optimal porous space of GAC-Alg immobilised beads by the microbes to promote stable biological activity for biohydrogen production. Hence, the study revealed that a combination of GAC and alginate as immobilised beads gave a positive response in bacterial immobilisation, especially in promoting the growth of hydrogen-producing bacteria during the fermentation [34]. The positive performance of the GAC-Alg beads was due to the presence of granule activated carbon inside, which acted as a support for the alginate carrier and maintained the stability of beads [21].…”

Section: Biohydrogen Production Of Immobilised Beads Gac-algmentioning

confidence: 94%

“…Generally, the differences in the shape of beads were caused by the gravity and surface tension imbalance when the beads dropped from the syringe. The beads shape formation also were affected by the viscosity of alginate and chitosan, and distance of dropper to gel solution [34]. This is a new method introduced to improve the stability of biofilm formation and adsorption capacity as well as enhanced the mechanical strength of the carrier, thus enhanced the hydrogen yield production.…”

Section: Bacteria Immobilisation In Gac-alg Entrapped With Chitosan Omentioning

confidence: 99%

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Effects of Alginate and Chitosan on Activated Carbon as Immobilisation Beads in Biohydrogen Production

et al. 2020

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In this study, the effects of alginate and chitosan as entrapped materials in the biofilm formation of microbial attachment on activated carbon was determined for biohydrogen production. Five different batch fermentations, consisting of mixed concentration alginate (Alg), were carried out in a bioreactor at temperature of 60 °C and pH 6.0, using granular activated carbon (GAC) as a primer for cell attachment and colonisation. It was found that the highest hydrogen production rate (HPR) of the GAC–Alg beads was 2.47 ± 0.47 mmol H2/l.h, and the H2 yield of 2.09 ± 0.22 mol H2/mol sugar was obtained at the ratio of 2 g/L of Alg concentration. Next, the effect of chitosan (C) as an external polymer layer of the GAC–Alg beads was investigated as an alternative approach to protecting the microbial population in the biofilm in a robust environment. The formation of GAC with Alg and chitosan (GAC–AlgC) beads gave the highest HPR of 0.93 ± 0.05 mmol H2/l.h, and H2 yield of 1.11 ± 0.35 mol H2/mol sugar was found at 2 g/L of C concentration. Hydrogen production using GAC-attached biofilm seems promising to achieve consistent HPRs at higher temperatures, using Alg as immobilised bead material, which has indicated a positive response in promoting the growth of hydrogen-producing bacteria and providing excellent conditions for microorganisms to grow and colonise high bacterial loads in a bioreactor.

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Revising the dark fermentative H2 research and development scenario – An overview of the recent advances and emerging technological approaches

Sekoai

Daramola

Mogwase

et al. 2020

Biomass and Bioenergy

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The indiscriminate use of fossil fuels has led to several challenges such as greenhouse gas emissions, environmental degradation, and energy security. Establishment of clean fuels is at the forefront of science and innovation in today's society to curb these problems. Dark fermentation (DF) is widely regarded as the most promising clean energy technology of the 21st century due to its desirable properties such as high energy content, its non-polluting features, its ability to use a broad spectrum of feedstocks and inoculum sources, as well as its ability to use mild fermentation conditions. In developing nations, this technology could be instrumental in establishing effective waste disposal systems while boosting the production of clean fuels. However, DF is still hindered by the low yields which stagnate its commercialization. This paper reviews the recent and emerging technologies that are gaining prominence in DF based on information that has been gathered from recent scientific publications. Herein, novel enhancement methods such as cell immobilization, nanotechnology, mathematical optimization tools, and technologies for biogas upgrading using renewable H 2 are comprehensively discussed. Furthermore, a section which discusses the potential of bioenergy in Sub-Saharan Africa including South Africa is included. Finally, scientific areas that need further research and development in DF process are also presented.

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Performance analysis of immobilized and co-immobilized enriched-mixed culture for hydrogen production

Cited by 7 publications

References 32 publications

Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System

Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System

Effects of Alginate and Chitosan on Activated Carbon as Immobilisation Beads in Biohydrogen Production

Revising the dark fermentative H2 research and development scenario – An overview of the recent advances and emerging technological approaches

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