Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System

Abstract

Biohydrogen production via dark fermentation is a prospective renewable energy technology. In the process, reused of immobilized mixed culture is very important as their activities greatly influencehydrogen production. The aim of this work was to evaluate the reuse of alginate beads affecting the biohydrogen production for 45 days. This study in batch reactor were performed using glucose 10 M as substrate, alginate and activated carbon as immobilization matrix materials, chicken eggshell as buffer, and cow dung biodigester as mixed culture. Hydrogen and pH on fermentation product are investigated by gas chromatography (GC) technique and pH meter, respectively. The colony diameter on immobilized and co-immobilized mixed culture was observed using optical microscope and colony diameter was measured using Image-Pro Plus Software v4.5.0.29. The surface morphology of immobilization and co-immobilization beads were determined using scanning electron microscope (SEM). The results showed that the colonies growth observed using optical microscopy or SEM was apparent only in the immobilization of mixed culture. The average growth and diameter of colonies per day were 90 colonies and 0.025 mm, respectively. The weight of beads and pH during the 45-day fermentation process for bead immobilization of mixed culture were 1.32–1.95 g and 6.25–6.62, correspondingly, meanwhile, the co-immobilizations of the mixed culture were 1.735–2.21g and 6.25–6.61, respectively. In addition, the average hydrogen volume of glucose fermented using an eggshell buffer and reusing the immobilization and co-immobilization beads was 18.91 mL for 15 cycles.©2020. CBIORE-IJRED. All rights reserved

Bioethanol Production from Oil Palm Empty Fruit Bunches Using Saccharomyces cerevisiae Immobilized on Sodium Alginate Beads

“…This phenomenon occurred because the lag phase time is shortened and the number of yeast cell inside Na-alginate beads is higher than that of the first fermentation cycle [34]. This is in accordance with several studies of reuse of alginate beads including being able to increase cell growth by up to 66 % in hydrogen production on the 9 th day [38], increasing ethanol production from carrot waste using S. cerevisiae immobilized in Na-alginate beads by 10 % [34] and ethanol production…”

“…Since the syringe needle diameter was very small (3.5 mm), a syringe adapter was placed between the syringe and syringe needle so that they were perfectly connected. The Na-alginate solution containing the yeast cells was discharged at the tip of the needle in the form of droplets [37,38]. The spherical Na-alginate beads were allowed to solidify in CaCl 2 solution as a result of ionic reaction between calcium and alginate under gentle agitation for 30 minutes using a magnetic stirrer [39].…”

A Review of Biohydrogen Productions from Lignocellulosic Precursor via Dark Fermentation: Perspective on Hydrolysate Composition and Electron-Equivalent Balance

“…For process intensification, the approaches such as pure culture, continuous operation, consolidated saccharification and fermentation, chemical additions, are optimization avenues. In this work, for convenience of discussions, the comparisons were divided into the following five categories, namely pure culture (PC) [73,[84][85][86][87][88][89][90][91][92][93][94], chemical additions (CA) [2,39,76,[95][96][97], continuous operation with mixed culture (COMC) [98][99][100][101][102], batch operation with mixed culture (BOMC) [103][104][105][106][107][108] and consolidated saccharification and fermentation (CSF) [18,31,61,[109][110][111], respectively. For quantitative comparison of hydrogen yield across different literatures, the necessary Carbon-molar-based mass balance for DF is pivotal and necessary.…”

Effect of Hydrogen Peroxide on Hydrogen Production from Melon Fruit (Cucumis melo L.) Waste by Anaerobic Digestion Microbial Community

“…The seed sludge was collected from biogas digester treating fruit waste at Gemah Ripah fruit market (Yogyakarta, Indonesia) for enriching the hydrogenproducing bacteria. The seeds were acidified to pH 3 by adding 2 M HCl and then maintaining for 24 hours and then adjusting back to pH 6.8 with the addition of 2 M NaOH (Amekan et al 2018;Damayanti et al 2020) to inactivate the hydrogen-consuming microbes before use in the enrichment of hydrogen-producing bacteria using glucose as the sole carbon source. The melon fruit waste used in this study was collected from Gemah Ripah fruit market located in Yogyakarta.…”