Perfluorooctanoic acid (PFOA) but not perfluorooctane sulfonate (PFOS) showed DNA damage in comet assay on Paramecium caudatum

Kawamoto, Kosuke; Oashi, Takahiro; Oami, Kazunori; Liu, Wei; Jin, Yinlong; Saito, Norimitsu; Sato, Itaru; Tsuda, Shuji

doi:10.2131/jts.35.835

Cited by 33 publications

(23 citation statements)

References 32 publications

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“…The different toxic effects of PFOA towards human and animals have been explored, such as reproductive toxicity, developmental toxicity, carcinogenicity, and immunotoxicity in experimental animals (Lau et al 2007;Olsen et al 2009). Additionally, according to previous studies, PFOA could inhibit the growth and development of macrophytes (Hanson et al 2005) and affect the survival of several invertebrates (Hazelton et al 2012;Kawamoto et al 2010). However, these toxicity studies of PFOA mainly focused on animals and plants, while the information on microorganisms is limited.…”

Section: Introductionmentioning

confidence: 84%

Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing

Yu-rong

Wang

Peng

et al. 2016

Environ Sci Pollut Res

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The characterization of bacterial community compositions and the change in perfluoroalkyl acids (PFAAs) along a natural river distribution system were explored in the present study. Illumina high-throughput sequencing was used to explore bacterial community diversity and structure in sediment polluted by PFAAs from the Xiaoqing River, the area with concentrated fluorochemical facilities in China. The concentration of PFAAs was in the range of 8.44-465.60 ng/g dry weight (dw) in sediment. Perfluorooctanoic acid (PFOA) was the dominant PFAA in all samples, which accounted for 94.2 % of total PFAAs. High-level PFOA could lead to an obvious increase in relative abundance of Proteobacteria, ε-Proteobacteria, Thiobacillus, and Sulfurimonas and the decrease in relative abundance of other bacteria. Redundancy analysis revealed that PFOA played an important role in the formation of bacterial community, and PFOA at higher concentration could reduce the diversity of bacterial community. When the concentration of PFOA was below 100 ng/g dw in sediment, no significant effect on microbial community structure was observed. Thiobacillus and Sulfurimonas were positively correlated with the concentration of PFOA, suggesting that both genera were resistant to PFOA contamination.

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Section: Introductionmentioning

confidence: 84%

Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing

Yu-rong

Wang

Peng

et al. 2016

Environ Sci Pollut Res

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“…A typical TP53 response involves differential p21/WAF1 expression, which was not observed, and is induced by DNA damage. Perfluorooctane sulfonate does not appear to be genotoxic [50][51][52], although Liu et al [34] did report DNA damage at high PFOS exposures but concluded that it was likely apoptosis related. Recent evidence has shown that lipid peroxidation products can activate TP53 [53], which may explain results from the present study.…”

Section: Interaction Network and Potential Regulatory Moleculesmentioning

confidence: 99%

Technical‐grade perfluorooctane sulfonate alters the expression of more transcripts in cultured chicken embryonic hepatocytes than linear perfluorooctane sulfonate

O’Brien

Austin

Williams

et al. 2011

Enviro Toxic and Chemistry

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Recently it was discovered that the perfluorooctane sulfonate (PFOS) detected in wildlife, such as fish-eating birds, had a greater proportion of linear PFOS (L-PFOS) than the manufactured technical product (T-PFOS), which contains linear and branched isomers. This suggests toxicological studies based on T-PFOS data may inaccurately assess exposure risk to wildlife. To determine whether PFOS effects were influenced by isomer content, we compared the transcriptional profiles of cultured chicken embryonic hepatocytes (CEH) exposed to either L-PFOS or T-PFOS using Agilent microarrays. At equal concentrations (10 µM), T-PFOS altered the expression of more transcripts (340, >1.5-fold change, p < 0.05) compared with L-PFOS (130 transcripts). Higher concentrations of L-PFOS (40 µM) were also less transcriptionally disruptive (217 transcripts) than T-PFOS at 10 µM. Functional analysis showed that L-PFOS and T-PFOS affected genes involved in lipid metabolism, hepatic system development, and cellular growth and proliferation. Pathway and interactome analysis suggested that genes may be affected through the RXR receptor, oxidative stress response, TP53 signaling, MYC signaling, Wnt/β-catenin signaling, and PPARγ and SREBP receptors. In all functional categories and pathways examined, the response elicited by T-PFOS was greater than that of L-PFOS. These data show that T-PFOS elicits a greater transcriptional response in CEH than L-PFOS alone and demonstrates the importance of considering the isomer-specific toxicological properties of PFOS when assessing exposure risk.

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“…Although not as well-studied as the P. aurelia complex of species, P. caudatum also has a long history of research (Calkins 1902;Sonneborn 1933). Recent studies on P. caudatum involve investigations into quorum sensing (Fellous et al 2012), thermal adaptation (Krenek et al 2012), learning (Armus et al 2006), endosymbiosis and parasite-mediated selection (Duncan et al 2010(Duncan et al , 2011, and ecotoxicology (Rao et al 2007;Kawamoto et al 2010;Hailong et al 2011). P. caudatum cells are larger than those of the aurelia complex and have a single, larger micronucleus, whereas aurelia species have two smaller micronuclei (Fokin 2010).…”

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confidence: 99%

Insights into Three Whole-Genome Duplications Gleaned from theParamecium caudatumGenome Sequence

et al. 2014

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Paramecium has long been a model eukaryote. The sequence of the Paramecium tetraurelia genome reveals a history of three successive whole-genome duplications (WGDs), and the sequences of P. biaurelia and P. sexaurelia suggest that these WGDs are shared by all members of the aurelia species complex. Here, we present the genome sequence of P. caudatum, a species closely related to the P. aurelia species group. P. caudatum shares only the most ancient of the three WGDs with the aurelia complex. We found that P. caudatum maintains twice as many paralogs from this early event as the P. aurelia species, suggesting that post-WGD gene retention is influenced by subsequent WGDs and supporting the importance of selection for dosage in gene retention. The availability of P. caudatum as an outgroup allows an expanded analysis of the aurelia intermediate and recent WGD events. Both the Guanine+Cytosine (GC) content and the expression level of preduplication genes are significant predictors of duplicate retention. We find widespread asymmetrical evolution among aurelia paralogs, which is likely caused by gradual pseudogenization rather than by neofunctionalization. Finally, cases of divergent resolution of intermediate WGD duplicates between aurelia species implicate this process acts as an ongoing reinforcement mechanism of reproductive isolation long after a WGD event.T HE genus Paramecium has been used as a model unicellular eukaryotic system for over a century, beginning with research by Jennings (1908) and leading to some of the earliest derivations of mathematical population-genetics properties (Jennings 1916(Jennings , 1917. The subsequent discovery of mating types in members of the Paramecium aurelia complex (Sonneborn 1937) permitted the first systematic crossbreeding of different genotypes in any unicellular eukaryote. Later work provided fundamental insights into major issues in biology, including mutagenesis (Igarashi 1966), molecular and developmental genetics (Sonneborn 1947), symbiosis (Beale et al. 1969), mitochondrial genetics (Adoutte and Beisson 1972), aging (Siegel 1967), nuclear differentiation (Berger 1937), and gene regulation (Allen and Gibson 1972). More recently, Paramecium has been used to study maternal inheritance (Nowacki et al. 2005), programmed genome rearrangements and transposon domestication (Arnaiz et al. 2012), epigenetic inheritance (Singh et al. 2014), and whole-genome duplication (Aury et al. 2006; Lynn McGrath, Jean-Francois Gout, Parul Johri, Thomas Graeme Doak, and Michael Lynch, unpublished results). Although not as well-studied as the P. aurelia complex of species, P. caudatum also has a long history of research (Calkins 1902;Sonneborn 1933). Recent studies on P. caudatum involve investigations into quorum sensing (Fellous et al. 2012), thermal adaptation (Krenek et al. 2012), learning (Armus et al. 2006), endosymbiosis and parasite-mediated selection (Duncan et al. 2010(Duncan et al. , 2011, and ecotoxicology (Rao et al. 2007;Kawamoto et al. 2010;Hailong et al. 2011). P...

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Perfluorooctanoic acid (PFOA) but not perfluorooctane sulfonate (PFOS) showed DNA damage in comet assay on Paramecium caudatum

Cited by 33 publications

References 32 publications

Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing

Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing

Technical‐grade perfluorooctane sulfonate alters the expression of more transcripts in cultured chicken embryonic hepatocytes than linear perfluorooctane sulfonate

Insights into Three Whole-Genome Duplications Gleaned from theParamecium caudatumGenome Sequence

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