Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing

Abstract

The characterization of bacterial community compositions and the change in perfluoroalkyl acids (PFAAs) along a natural river distribution system were explored in the present study. Illumina high-throughput sequencing was used to explore bacterial community diversity and structure in sediment polluted by PFAAs from the Xiaoqing River, the area with concentrated fluorochemical facilities in China. The concentration of PFAAs was in the range of 8.44-465.60 ng/g dry weight (dw) in sediment. Perfluorooctanoic acid (PFOA) was the dominant PFAA in all samples, which accounted for 94.2 % of total PFAAs. High-level PFOA could lead to an obvious increase in relative abundance of Proteobacteria, ε-Proteobacteria, Thiobacillus, and Sulfurimonas and the decrease in relative abundance of other bacteria. Redundancy analysis revealed that PFOA played an important role in the formation of bacterial community, and PFOA at higher concentration could reduce the diversity of bacterial community. When the concentration of PFOA was below 100 ng/g dw in sediment, no significant effect on microbial community structure was observed. Thiobacillus and Sulfurimonas were positively correlated with the concentration of PFOA, suggesting that both genera were resistant to PFOA contamination.

Fungal biotransformation of 6:2 fluorotelomer alcohol

“…However, the effects of perfluoroalkyl substances on fungal stress responses and the fungal transformation of 6:2 FTOH and related compounds has not yet been examined. Comparatively, bacteria (e.g., Dehalococcoides and Rhodococcus jostii RHA1) were negatively impacted by perfluoroalkyl acids (Weathers, Harding‐Marjanovic, Higgins, Alvarez‐Cohen, & Sharp, ; Weathers, Higgins, & Sharp, ), and the bacterial community in aerobic river sediment was observed to decrease in diversity with PFOA exposure (>100 nanograms per gram dry weight in sediment; Sun, Wang, Peng, Wang, & Lu, ). Hydrogenotrophic methanogens from anaerobic sludge were also inhibited by 500 mg PFOS/L, but other PFASs tested, such as PFBS, PFPrA, and PFPeA, did not inhibit methanogenesis (Ochoa‐Herrera, Field, Luna‐Velasco, & Sierra‐Alvarez, ).…”

“…Effect of PFOA and PFOS on growth of fungal isolates PFOA and PFOS by the six fungal isolates was examined. Fungi wereSharp, 2015), and the bacterial community in aerobic river sediment was observed to decrease in diversity with PFOA exposure (>100 nanograms per gram dry weight in sediment;Sun, Wang, Peng, Wang, & Lu, 2016). Hydrogenotrophic methanogens from anaerobic sludge were also inhibited by 500 mg PFOS/L, but other PFASs tested, such as PFBS, PFPrA, and PFPeA, did not inhibit methanogene-…”

Direct effects of organic pollutants on the growth and gene expression of the Baltic Sea model bacteriumRheinheimerasp.BAL341

“…strain CPB4337 (Rosal et al ., ), cause membrane disruption, oxidative stress and DNA damage in Escherichia coli bacteria (Liu et al ., ), and that they influence bacterial membrane fluidity, permeability and quorum sensing (Fitzgerald et al ., , ). Furthermore, there is a 16S rRNA gene survey indicating that these compounds can drive selective changes in bacterial sediment communities (Sun et al ., ). In the current study, we found no growth responses of bacteria in exponential phase to the combination of PFOS and PFOA and only a very minor difference for bacteria in stationary phase.…”

Traditional and new POPs in environments along the Bohai and Yellow Seas: An overview of China and South Korea

“…Concentrations of PFOA in water from the Xiaoqing River basin ranged from 38.6 to 1,707,290 ng L À1 (Liu et al, 2016). The greatest concentrations of PFASs in sediment were detected in the Xiaoqing River, of which PFOA was dominant and its concentrations were between 3.86 and 456.20 ng g À1 dw (Sun et al, 2016). In addition, studies in the Liaohe, Haihe and Yellow Rivers were also carried out (Li et al, 2011;Pan et al, 2011b;Wang et al, 2012aWang et al, , 2012bYang et al, 2011;Zhao et al, 2013).…”