Perfluorocarbon Effects on Rabbit Blood-Retinal Barrier Permeability

Abstract

Studies have been made of the effects of intravitreal perfluoro-n-octane on the permeability to fluorescein of the blood-retinal barrier in rabbits. At day 1 after injection, there is increased aqueous humor fluorescence that reflects the physical disturbance to the eye following injection. From that time through 7 weeks, there is no evidence of any overt toxicity to the blood-retinal barrier. The retention in the vitreous of a small volume of perfluoro-n-octane following its intraoperative use would not be expected to induce a toxic response. Some effect on an already compromised retina cannot, however, be excluded.

Avaliação do possível efeito tóxico de um alcano semifluorinado de uso oftalmológico sobre cultura de células Vero

“…Embora o F 6 H 8 tenha sido testado quanto a sua toxicidade em cultura de células e não tenha demonstrado inibir a proliferação celular (29) , este produto ainda não foi avaliado tendo como parâmetro um efeito compressivo inerte. Isto permitiu uma observação paralela dos achados encontrados no experimento.…”

“…O fato de não se encontrarem, no entanto, diferenças marcantes nos resultados, sugerem que o F 6 H 8 não apresenta outro mecanismo de ação, que não o efeito compressivo, para as alterações encontradas. Esses dados então em acordo com outros relatos previamente publicados (29) . Em conjunto, estes resultados apontam para a interpretação de que as alterações estruturais provocadas pelo F 6 H 8 em olhos após cirurgias em animais de experimentação seriam principalmente em decorrência da força mecânica do mesmo, exercida sobre os tecidos no qual estão em contato e tempodependente.…”

Effect of perfluorodecalin on human retinal pigment epithelium and human corneal endothelium in vitro

“…Inflammatory reactions could be detected in rabbit eyes after 2-12 weeks contact with PFCLs. In almost all eyes subendothelial vascularization and scar formation in the periphery of the perfluorodecalin bleb could be observed at the end of the study [22,14]. It is therefore assumed that the loss of cell density as well as the morphological changes may be caused by mechanical or barrier effects of perfluorodecalin.…”

“…PFCL are usually removed at the end of the operation but small amounts may remain in the eye [24]. Recently, contradictory findings have been reported regarding damage in association with residual PFCL in the eye [3,10,14,15,22,24].…”

New substances for intraocular tamponades: perfluorocarbon liquids, hydrofluorocarbon liquids and hydrofluorocarbon-oligomers in vitreoretinal surgery

“…Intraocular droplet formation does occur within the first few days, demonstrated here in a rabbit eye 5 days after injection. Simultaneously, flakey white precipitates appear in the residual vitreous and build membrane like structures around the bubbles, which may prevent reconfluence and indicate disturbance of the blood retinal barrier and leakage of protein ( Fig.1) [20]. Experimentally, functional and morphological alterations of the retinal blood supply were found in two respects: first, an immediate vasoconstriction with an up to 30% reduction in retinal blood flow was measured in rabbit eyes after injection of PFCL [25].…”