Perfil Fitoquímico E Potencial Antioxidante De Extratos Etanólicos Da Espécie Bauhinia Monandra Kurz (Fabaceae) / Phytochemical Profile and Antioxidant Potential of Ethanol Extracts of the Species Bauhinia Monandra Kurz (Fabaceae)

Oliveira, Daniel Pereira de; Cavalcanti, Eveline Solon Barreira; Morais, Selene Maia de; Pinto, Cleonilda Claita Carneiro; Montes, Renato Almeida; Santos, Lucas Mendes Brito dos; Furtado, Milena Lira; Silva, Eduardo Souza da

doi:10.34117/bjdv6n11-176

Cited by 1 publication

(1 citation statement)

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“…The C. brasiliensis and D. violacea seeds were collected from plants located at Crato, Ceará, Brazil, and the ConBR and DVL lectins were purified as previously described Lectins isolated from plants have been used as biotechnological tools against several infectious diseases [29], demonstrating potent antibacterial and antiviral effects [30][31][32][33]. In the antiviral context, lectins isolated from seeds of Bauhinia variegata (specificity for glucose and galactose) demonstrated activity against Coxsackievirus B3 and Rotavirus [34,35]; from Momordica charantia (specificity for galactose and N-Acetylgalactosamine) against HIV, HSV-1, H1N1, H3N2, and H5N1 [36][37][38][39]; from Mucuna pruriens (specificity for mannose) against HCV [40]; and from Senna tora (specificity for mannose and galactose) against SARS-CoV-2 [41,42]. Therefore, we hypothesized that the mannose-biding lectins ConBR and DVL could present antiviral activity against SARS-CoV-2, potentially by interacting with the S protein to abrogate virus cell entry.…”

Section: Extraction and Purification Of Conbr And Dvlmentioning

confidence: 99%

Mannose-Binding Lectins as Potent Antivirals against SARS-CoV-2

Grosche,

Souza,

Ferreira

et al. 2023

Viruses

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The SARS-CoV-2 entry into host cells is mainly mediated by the interactions between the viral spike protein (S) and the ACE-2 cell receptor, which are highly glycosylated. Therefore, carbohydrate binding agents may represent potential candidates to abrogate virus infection. Here, we evaluated the in vitro anti-SARS-CoV-2 activity of two mannose-binding lectins isolated from the Brazilian plants Canavalia brasiliensis and Dioclea violacea (ConBR and DVL). These lectins inhibited SARS-CoV-2 Wuhan-Hu-1 strain and variants Gamma and Omicron infections, with selectivity indexes (SI) of 7, 1.7, and 6.5, respectively for ConBR; and 25, 16.8, and 22.3, for DVL. ConBR and DVL inhibited over 95% of the early stages of the viral infection, with strong virucidal effect, and also protected cells from infection and presented post-entry inhibition. The presence of mannose resulted in the complete lack of anti-SARS-CoV-2 activity by ConBR and DVL, recovering virus titers. ATR-FTIR, molecular docking, and dynamic simulation between SARS-CoV-2 S and either lectins indicated molecular interactions with predicted binding energies of −85.4 and −72.0 Kcal/Mol, respectively. Our findings show that ConBR and DVL lectins possess strong activities against SARS-CoV-2, potentially by interacting with glycans and blocking virus entry into cells, representing potential candidates for the development of novel antiviral drugs.

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