Perfil de polifenoles, capacidad antioxidante y efecto citotóxico in vitro en líneas celulares humanas de un extracto hidroalcohólico de pétalos de Calendula officinalis L.

Abstract

ARTÍCULO ORIGINAL abstractThe hydro-alcoholic extract from Calendula officinalis petals, was evaluated showing beneficial effects against oxidative stress and therefore on diseases associated to inflammatory processes. The content of polyphenols of the extract was determined by TLC and HPLC-UV-DAD and the antioxidant efficiency estimated from Fe 3+ /Fe 2+ reducing power, Cu 2+and Fe 2+ chelation,• OH radical scavenging, ABTS [2, 2´-azino bis (3 ethylbenzothiazoline-6-sulphonic acid)] and DPPH (2,2´diphenyl-1-picrylhydrazyl) assays. The in vitro growth inhibition (IC 50 ) in human cancer cell breast (MDA-MB-231), uterine cervix (HeLa), lung (A-549), colon (HT-29) and (Caco-2) was determined by MTT method. The extract showed that the polyphenols content in the hydro-alcoholic extract was 37.01±0.015 mg GAE g -1 , being rutin the most abundant one. ABTS TEAC and DPPH TEAC tests showed a high antioxidant capacity. The antioxidant activity profile displayed high reducing capacity and ability to chelate Cu 2+ and Fe 2+ as well as good• OH radical scavenging capacity. The extract showed a cytotoxic effect on human cancer cell lines of colon (HT-29) and (Caco-2), breast (MDA-MB-231), uterine cervix (HeLa), and lung (A-549) (IC 50 = 10.84, 11.73, 11.26, 11.53, and 11.96 µg mL -1 , respectively). The correlation analysis suggests that the Calendula officinalis polyphenols are directly related to the antioxidant efficiency of the extract and inversely to the cell viability.Key Words: Calendula officinalis, polyphenols, antioxidant, cytotoxicity. OH, y ensayos ABTS [2, 2´-azino-bis (3 etilbenzotiazolin-6-sulfónico) y DPPH (2,2´difenil-1-picrilhidracilo). La inhibición del crecimiento in vitro (IC 50 ) en células cancerosas humanas de mama (MDA-MB-231), de cuello uterino (HeLa) , pulmón (A-549) , y colon (HT-29 y Caco-2), fue determinado por el método de MTT. El extracto mostró que el contenido total de polifenoles fue de 37.01±0.015 mg GAe g -1 , siendo la rutina la más abundante. Las pruebas ABTS TEAC y DPPH TEAC mostraron una alta capacidad antioxidante. El perfil de actividad antioxidante mostró buena capacidad reductora y capacidad para quelar Cu 2+ y Fe 2+, así como una buena capacidad de eliminación de radicales • OH. El extracto también mostró efecto citotóxico en células de colon (HT-29 y Caco-2) , de mama (MDA-MB-231), de cuello uterino (HeLa) y pulmón (A-549) (IC 50 = 10.84, 11.73, 11.26, 11.53 y 11.96 μg mL -1 , respectivamente). El análisis de correlación sugiere que los polifenoles de Calendula officinalis están directamente relacionados con la capacidad antioxidante del extracto y se asocian inversamente con la viabilidad celular.Palabras Clave: Calendula officinalis, polifenoles, antioxidante, citotoxicidad.

In vitro Fermentation of Polysaccharides from Aloe vera and the Evaluation of Antioxidant Activity and Production of Short Chain Fatty Acids

“…A BEH C18 Column (2.1 mm X 100 mm) was used and the chromatograms were registered through detection of the Total Ion Current. The MS conditions of analysis in negative-ion mode were as follows: drying gas (nitrogen) flow rate, 8 L/min; gas temperature, 180 °C; scan range, m/z 50–3000; capillary voltage, 4500 V; and nebulizer pressure, 2.5 bar [44]. A solution of 8 mg/mL of lyophilized AV was prepared, homogenized, and passed through a 0.22 µm cellulose filter prior to analysis.…”

Cuantificación de fenoles y flavonoides totales en un extracto blando de flores de Calendula officinalis Linn

“…Su esqueleto carbonado contiene 15 carbonos ordenados en dos anillos aromáticos unidos por un puente de tres carbonos (heterociclo). Se clasifican en función del grado de oxidación del puente de tres carbonos, siendo los principales: antocianinas, flavonas, flavonoles (quercetina, la miricetina, kaempferol), isoflavonas, flavanoles (catequina y derivados) (Hernández-Rosas, et al, 2018).…”

Polyacrylamide Hydrogel Containing Calendula Extract as a Wound Healing Bandage: In Vivo Test

“…The presence of phenols in the species contributes to its high antioxidant potential [ 46 , 47 ]. Calendula has widespread therapeutic applications, which include tissue re-epithelialization and general wound healing action.…”