Percutaneous Radiofrequency Thermal Ablation of Lung VX2 Tumors in a Rabbit Model Using a Cooled Tip-Electrode

Lee, Jeong Min; Jin, Gong Yong; Li, Chun Ai; Chung, Gyung Ho; Lee, Sang Yong; Han, Young Min; Chung, Myoung Ja; Kim, Chong Soo

doi:10.1097/00004424-200302000-00008

Cited by 34 publications

(7 citation statements)

References 32 publications

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“…As the electrode is placed percutaneously directly into the tumor under cross-sectional imaging guidance, such as chest CT, complications such as pneumothorax occur at relatively high rates. The frequency of pneumothorax or hemothorax with use of percutaneous direct electrode placement was 47% in a rabbit model [9]. Clinical complications occurring in percutaneously directly RFA were 16%–35% [3, 4].…”

Section: Discussionmentioning

confidence: 99%

Clinical Experience of Bronchoscopy-Guided Radiofrequency Ablation for Peripheral-Type Lung Cancer

Koizumi

Kobayashi

Tanabe

et al. 2013

Case Reports in Oncological Medicine

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We have developed a new internal cooled electrode for radiofrequency ablation (RFA) (Japan Application no. 2006-88228) suitable for forceps channel bronchoscopy. Here, we present our clinical experience with bronchoscopy-guided RFA under computed tomography (CT) monitoring for patients with peripheral-type non-small-cell lung cancer (NSCLC). Bronchoscopy-guided RFA was performed in two patients (80 and 70 years old) with NSCLC, who had no lymph node involvement and distant metastases (T1N0M0), but not indicated for surgery because of other complications, such as advanced age, poor pulmonary function, and refusal of thoracic surgery. The locations of the tumors were right S2 and left S3, respectively. Although the tumors showed ground-glass opacity (GGO) with solid components in both cases, radiographic findings changed to reduced mass-like shadow and remained stable for 4 and 3.5 years after bronchoscopy-guided RFA. As the former case developed progressive disease on chest CT, bronchoscopy-guided RFA was repeated in the same lesion, resulting in no change for the subsequent 1 year. There were no adverse reactions during the procedures. Thus, bronchoscopy-guided RFA is a safe and feasible procedure that represents a potentially useful therapeutic tool in local control in medically inoperable patients with stage I NSCLC.

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Section: Discussionmentioning

confidence: 99%

Clinical Experience of Bronchoscopy-Guided Radiofrequency Ablation for Peripheral-Type Lung Cancer

Koizumi

Kobayashi

Tanabe

et al. 2013

Case Reports in Oncological Medicine

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“…Studies performing CT-guided (rather than real-time CT fluoroscopy-guided) VX2 tumor cell inoculations with or without Matrigel and with 18- or 21-gauge needles have reported successful formation rates of VX2 lung cancer of 45.8–77.8%; however, multiple rather than solitary lesions were obtained [25–27]. Similar to the procedures performed in previous studies, we first percutaneously injected VX2 tissue suspensions without Matrigel using an 18-gauge needle under real-time CT fluoroscopy guidance (group 1).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies of lung cancer have reported CT-guided inoculations of VX2 tumor cell suspensions [25–27]. However, the success rate of such inoculations in lung cancer models of solitary tumors was low because injection of the suspension into the lung parenchyma was difficult to confirm in real time.…”

Section: Introductionmentioning

confidence: 99%

Real-time computed tomography fluoroscopy-guided solitary lung tumor model in a rabbit

Choi

Young

et al. 2017

PLoS ONE

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Preclinical studies of lung cancer require suitable large-animal models to allow evaluation and development of surgical and interventional techniques. We assessed the feasibility and safety of a novel rabbit lung cancer model of solitary tumors, in which real-time computed tomography fluoroscopy is used to guide inoculation of VX2 carcinoma single-cell suspensions. Thirty-eight rabbits were divided into four groups according to the volume of the VX2 tissue or cell suspension, the volume of lipiodol, the volume of Matrigel, and the injection needle size. The mixtures were percutaneously injected into rabbit lungs under real-time computed tomography fluoroscopy guidance. Two weeks later, VX2 lung carcinomas were confirmed via positron emission tomography/computed tomography, necropsy, and histology. Real-time computed tomography fluoroscopy allowed the precise inoculation of the tumor cell suspensions containing lipiodol, while the use of Matrigel and a small needle prevented leakage of the suspensions into the lung parenchyma. Solitary lung tumors were successfully established in rabbits (n = 22) inoculated with single-cell suspensions (150 μL), lipiodol (150 μL), and Matrigel (150 μL) using a 26-gauge needle. This combination was determined to be optimal. Pneumothorax was observed in only two of the 38 rabbits (5.3%), both of which survived to the end of the study without any intervention. Real-time computed tomography fluoroscopy-guided inoculation of VX2 single-cell suspensions with lipiodol and Matrigel using a small needle is an easy and safe method to establish solitary lung tumors in rabbits.

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“…This is further complicated by heterogeneity in the time from inoculation to sacrifice (ranging from days to months). It is known that VX2 lung tumor models can generate nodal metastases, but this has been largely demonstrated on autopsy after animals died from uncontrolled disease at 26 to around 40 days [12, 13]. Those studies that stratified rabbits by time from inoculation did so for assessing changes on imaging or responses to radiofrequency ablation; the timing of nodal metastasis development was therefore unclear [12, 14].…”

Section: Introductionmentioning

confidence: 99%

Rabbit VX2 lung tumor models can form early nodal metastases

et al. 2019

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BackgroundThe rabbit squamous cell cancer line, VX2, has been used to generate various tumor models in rabbits. It is notable for its ability to generate nodal metastases. However, the timing and extent of nodal metastases vary by primary inoculation site and methodology. The development of metastases specifically in lung cancer models has not been well-described. We sought to characterize the generation of nodal metastases in rabbit transbronchial VX2 lung tumor models.MethodsRabbit VX2 lung tumor models were created in the right lung via transbronchial injection and serially imaged by computed tomography. Rabbits (n = 15) were sacrificed from between 5 and 24 days post-inoculation for collection of the ipsilateral and contralateral paratracheal lymph nodes. These underwent histopathological evaluation for metastases using hematoxylin and eosin as well as cytokeratin AE1/AE3 immunohistochemical staining.ResultsNodal metastases were detectable as early as 1 week after inoculation but were more prevalent with longer inoculation; all rabbits at > 2 weeks post-inoculation had nodal metastases. Contralateral metastases were in general seen later than ipsilateral metastases. Lymph node volume did not predict the likelihood of nodal metastases (p = 0.4 and p = 0.07 for ipsilateral and contralateral nodal metastases, respectively), but primary tumor volume was significantly associated with the likelihood of nodal metastases (p = 0.001 and p = 0.005 for ipsilateral and contralateral nodal metastases, respectively). Ipsilateral metastases were detectable at a tumor diameter of 1 cm; contralateral metastases were more variable but in general required a tumor diameter of 2 cm.ConclusionsRabbit transbronchial VX2 lung tumor models generate nodal metastases relatively early after inoculation. These results suggest such models may be valuable tools in the investigation of novel therapeutic modalities relevant for the treatment of both early-stage and locally advanced lung cancer.

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Percutaneous Radiofrequency Thermal Ablation of Lung VX2 Tumors in a Rabbit Model Using a Cooled Tip-Electrode

Abstract: This experimental study demonstrates the feasibility of RFA therapy for treating lung VX2 tumors in rabbits, although RFA for central tumors carries the potential for major complications, including large pneumothorax or obstructive pneumonia.

Cited by 34 publications

References 32 publications

Clinical Experience of Bronchoscopy-Guided Radiofrequency Ablation for Peripheral-Type Lung Cancer

Clinical Experience of Bronchoscopy-Guided Radiofrequency Ablation for Peripheral-Type Lung Cancer

Real-time computed tomography fluoroscopy-guided solitary lung tumor model in a rabbit

Rabbit VX2 lung tumor models can form early nodal metastases

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