Real-time computed tomography fluoroscopy-guided solitary lung tumor model in a rabbit

Abstract

Preclinical studies of lung cancer require suitable large-animal models to allow evaluation and development of surgical and interventional techniques. We assessed the feasibility and safety of a novel rabbit lung cancer model of solitary tumors, in which real-time computed tomography fluoroscopy is used to guide inoculation of VX2 carcinoma single-cell suspensions. Thirty-eight rabbits were divided into four groups according to the volume of the VX2 tissue or cell suspension, the volume of lipiodol, the volume of Matrigel, and the injection needle size. The mixtures were percutaneously injected into rabbit lungs under real-time computed tomography fluoroscopy guidance. Two weeks later, VX2 lung carcinomas were confirmed via positron emission tomography/computed tomography, necropsy, and histology. Real-time computed tomography fluoroscopy allowed the precise inoculation of the tumor cell suspensions containing lipiodol, while the use of Matrigel and a small needle prevented leakage of the suspensions into the lung parenchyma. Solitary lung tumors were successfully established in rabbits (n = 22) inoculated with single-cell suspensions (150 μL), lipiodol (150 μL), and Matrigel (150 μL) using a 26-gauge needle. This combination was determined to be optimal. Pneumothorax was observed in only two of the 38 rabbits (5.3%), both of which survived to the end of the study without any intervention. Real-time computed tomography fluoroscopy-guided inoculation of VX2 single-cell suspensions with lipiodol and Matrigel using a small needle is an easy and safe method to establish solitary lung tumors in rabbits.

Tumor-Targeting Glycol Chitosan Nanoparticles for Image-Guided Surgery of Rabbit Orthotopic VX2 Lung Cancer

“…For the establishment of the rabbit orthotopic VX2 lung tumor model, the VX2 tumor cell/matrigel/lipiodol mixture was directly injected to the lateral chest region using computed tomography (CT, Brillance 64; Philips, Amsterdam, The Netherlands)-guided interventional techniques [35]. In brief, a 26-gauge needle was inserted into the peripheral lung region under the guidance of real-time CT fluoroscopy images after the administration of local anesthesia.…”

Correction: Real-time computed tomography fluoroscopy-guided solitary lung tumor model in a rabbit

“…The correct names are: Ji Yun Rho and Hyun Koo Kim. The correct citation is: Choi BH, Young HS, Quan YH, Rho JY, Eo JS, Han KN, et al (2017) Real-time computed tomography fluoroscopy-guided solitary lung tumor model in a rabbit. PLoS ONE 12(6): e0179220.…”

Establishment of a modified percutaneous CT-guided paraspinal intramuscular VX-2 squamous cell carcinoma dual tumor model in rabbits

“…Still, in practice, this method often fails to obtain a tumor model of stable size and location (Lee et al, 2009). Choi et al (2017) developed a novel technique for creating a solitary VX-2 lung tumor in a rabbit lung cancer model. The technique involves inoculating a lipiodoland Matrigel-containing tumor cell suspension into rabbits using a small needle under real-time CT fluoroscopy guidance.…”

“…The novel method is an easy and safe method for successfully forming VX-2 solitary lung tumors in rabbits. This model will help surgical practice and interventional radiology studies of lung cancers (Choi et al, 2017).…”