Percutaneous Ethanol Injection of the Prostate as Minimally Invasive Treatment for Benign Prostatic Hyperplasia: Preliminary Report

Savoca, G.; Stefani, Stefano De; Gattuccio, Ignazio; Paolinelli, D; Stacul, Fulvio; Belgrano, E.

doi:10.1159/000049826

Cited by 21 publications

(23 citation statements)

References 13 publications

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“…3,15 The procedure itself is easy, safe, and rapid; it may be of special importance in managing medically high-risk patients who are not candidates for other surgical modalities.…”

Section: Discussionmentioning

confidence: 99%

Transurethral Ethanol Ablation of the Prostate for Symptomatic Benign Prostatic Hyperplasia: Long-Term Follow-Up

El‐Husseiny

Buchholz²

2011

Journal of Endourology

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“…3,15 The procedure itself is easy, safe, and rapid; it may be of special importance in managing medically high-risk patients who are not candidates for other surgical modalities.…”

Section: Discussionmentioning

confidence: 99%

Transurethral Ethanol Ablation of the Prostate for Symptomatic Benign Prostatic Hyperplasia: Long-Term Follow-Up

El‐Husseiny

Buchholz²

2011

Journal of Endourology

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“…Additionally, transperineal injection of anhydrous ethanol has received limited attention in the literature. Promising results were demonstrated with only small experiences with short term follow-up using TRUS [12].…”

Section: Procedural Evolutionmentioning

confidence: 99%

Injectables in the prostate

Saemi

Plante

2008

Current Opinion in Urology

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“…[30][31][32][33][34] Clinical studies examining the benefit of prostatic injection with ethanol for symptomatic BPH have demonstrated significant postsurgical improvements. [35][36][37] Furthermore, ethanol promotes tunicamycin-and thapsigargin-induced neuronal cell death, and potentiates TRAIL-induced apoptosis in colon cancer cells and leukemic T-Lymphocytes. 38,39 However, little is known about its use as a chemosensitizing agent in PCa cells.…”

Section: Introductionmentioning

confidence: 99%

Ethanol promotes cytotoxic effects of tumor necrosis factor-related apoptosis-inducing ligand through induction of reactive oxygen species in prostate cancer cells

Plante

Arscott

Folsom

et al. 2012

Prostate Cancer Prostatic Dis

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BACKGROUND: Effective treatment of prostate cancer (PCa) remains a major challenge due to chemoresistance to drugs including tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Ethanol and ethanol extracts are known apoptosis inducers. However, cytotoxic effects of ethanol on PCa cells are unclear. METHODS: In this study we utilized PC3 and LNCaP cell culture models. We used immunohistochemical analysis, western blot analysis, reactive oxygen species (ROS) measurement, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) Cell Proliferation Assay, Annexin-V staining and flow cytometry for quantification of apoptosis. In vitro soft agar colony formation and Boyden chamber invasion assays were used. Tumorigenicity was measured in a xenotransplantation mouse model. RESULTS: Here, we demonstrate that ethanol enhances the apoptosis-inducing potential of TRAIL in androgen-resistant PC3 cells and sensitizes TRAIL-resistant, androgen sensitive LNCaP cells to apoptosis through caspase activation, and a complete cleavage of poly (ADP)-ribose polymerase, which was in association with increased production of ROS. The cytotoxicity of ethanol was suppressed by an antioxidant N-acetyl cystein pretreatment. Furthermore, ethanol in combination with TRAIL increased the expression of cyclin-dependent kinase inhibitor p21 and decreased the levels of Bcl-2 and phosphorylated-AKT. These molecular changes were accompanied by decreased proliferation, anchorage-independent growth and invasive potential of PC3 and LNCaP cells. In vivo studies using a xenotransplantation mouse model with PC3 cells demonstrated significantly increased apoptosis in tumors treated with ethanol and TRAIL in combination. CONCLUSIONS: Taken together, use of ethanol in combination with TRAIL may be an effective strategy to augment sensitivity to TRAIL-induced apoptosis in PCa cells.

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Percutaneous Ethanol Injection of the Prostate as Minimally Invasive Treatment for Benign Prostatic Hyperplasia: Preliminary Report

Cited by 21 publications

References 13 publications

Transurethral Ethanol Ablation of the Prostate for Symptomatic Benign Prostatic Hyperplasia: Long-Term Follow-Up

Transurethral Ethanol Ablation of the Prostate for Symptomatic Benign Prostatic Hyperplasia: Long-Term Follow-Up

Injectables in the prostate

Ethanol promotes cytotoxic effects of tumor necrosis factor-related apoptosis-inducing ligand through induction of reactive oxygen species in prostate cancer cells

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