Percentage Value of Membrane Integrity and Acrosome Integrity Spermatozoa in Simmental Liquid Semen with Addition Penicillin and Sweet Orange Essential Oil

Sitepu, Sukma Aditya; Marisa, Julia

doi:10.1088/1755-1315/327/1/012027

Cited by 7 publications

(8 citation statements)

References 5 publications

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“…The results of previous studies by adding a combination of penicillin and essential oils have been shown to increase the percentage of membrane integrity in Simmental spermatozoa [4]. In this research, the combination of gentamicin and the sweet orange essential oil is expected to further increase the percentage of whole spermatozoa membrane integrity in Simmental liquid semen.…”

Section: One Of the Success Factors Of Artificialmentioning

confidence: 66%

Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

Sitepu¹,

Marisa²

2020

AJARR

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Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender. Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration. Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%. Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.

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Section: One Of the Success Factors Of Artificialmentioning

confidence: 66%

Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

Sitepu¹,

Marisa²

2020

AJARR

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“…Sweet orange essential oil containing limonene and linalool are toxic to bacteria [9]. The results of previous studies by adding a combination of penicillin and Sweet orange essential oil have been shown to increase the percentage of intact plasma membranes in Simmental spermatozoa [10]. In this research, a combination of gentamicin and sweet orange essential oil is expected to increase the percentage of spermatozoa acrosome integrity in Simmental bull liquid semen.…”

Section: One Of the Important Factors Of Artificialmentioning

confidence: 72%

“…From the data obtained, it has been shown that the addition of sweet orange essential oil increases the value of acrosome integrity spermatozoa Simmental bull percentage before semen equilibration. The higher level of administration of sweet orange essential oil will further increase the value of acrosome integrity spermatozoa [10]. Since it perhaps enhances membrane integrity and thus prevents cold shock.…”

Section: Resultsmentioning

confidence: 99%

Acrosome Integrity Spermatozoa Test with Addition of Gentamicin and Sweet Orange Essential Oil in Simmental Bull Liquid Semen

Sitepu

Marisa

2020

AJARR

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Aims: The purpose of this study was to determine the percentage value of spermatozoa acrosome integrity with the addition of gentamicin and sweet orange essential oil on the tris yolk extender on Simmental bull liquid semen. Methodology: The material that was used in this research included Simmental bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender prepares with Tris (hydroxymethyl) aminomethane (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aqua dest (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replications. The treatments comprised the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1% inclusion levels. The parameter observed was the evaluation of the percentage value of acrosome integrity before and after equilibration equilibration. Results: The results showed that the percentage value of acrosome integrity in spermatozoa linearly increased along with the addition of sweet orange essential oil. The addition of sweet orange essential oil had a very significant effect (P <0.01) on the percentage of spermatozoa acrosome integrity in Simmental bull liquid semen. The percentage of acrosome integrity on the addition of 0% (P0) sweet orange essential oil is 60%, the addition of 0.25% (P1) is 64%,the addition of 0.5% (P2) is 67%, the addition of 0.75% (P3) is 69%, and the addition of 1% (P4) is 73%. Conclusion: The addition of 1% (P4) sweet orange essential oil can increase the value of acrosome integrity on spermatozoa, which subsequently improve the quality of Simmental bull liquid semen.

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“…(2018) with 40% found in Bali bull and 50% in Simmental bull during the observation of the hood acrosome intact. Sitepu and Marisa (2019) claimed that the acrosome plays an important role in the fertilization process for successful artificial insemination. The spermatozoa binding initiation to the zona pellucida will trigger the acrosome reaction which lead to the release and activation of acrosome enzymes, so that the spermatozoa are able to penetrate the zona pellucida.…”

Section: The Quality Of Frozen Semen Of Bali and Simmental Bullmentioning

confidence: 99%

Bali Bull, Fresh Semen, Frozen Semen, Semen Quality, Simmental Bull

Adiputra¹,

Maulana²,

Kaiin³

et al. 2022

AAVS

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This study was conducted to determine the quality fresh and frozen semen of Bali and Simmental bull in the Technical Implementation Unit of Regional Artificial Insemination Center at Pucak, South Sulawesi. Two Bali bulls and two Simmental bulls aged 5-6 were used. The parameters observed in the fresh semen were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were motility, viability, abnormality, intact plasma membrane, acrosome status, DNA integrity, and movement patterns. Viability and abnormality parameters were stained with eosin-nigrosine, intact plasma membrane was stained with Hypo-osmotic (HOS) solution, acrosome status was stained with peanut agglutinin (PNA), and DNA integrity was stained with acridine orange (AO). The results showed that the volume, pH, motility, abnormality, intact plasma membrane, and concentration of fresh semen of Bali and Simmental bull were not significantly different (P>0.05). Likewise, the frozen semen of Bali and Simmental breed did not show any significant differences (P>0.05) in terms of motility, acrosome status, intact plasma membrane, DNA integrity, and movement patterns, but the viability of Bali bull was significantly higher than that of Simmental bull and the abnormality of Simmental bull was significantly higher than that of Bali bull. Based on the results, it can be concluded both Bali and Simmental bull has the same quality of fresh semen, but Bali bull had better viability and abnormality compared to those Simmental breed of frozen semen.

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Percentage Value of Membrane Integrity and Acrosome Integrity Spermatozoa in Simmental Liquid Semen with Addition Penicillin and Sweet Orange Essential Oil

Cited by 7 publications

References 5 publications

Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

Acrosome Integrity Spermatozoa Test with Addition of Gentamicin and Sweet Orange Essential Oil in Simmental Bull Liquid Semen

Bali Bull, Fresh Semen, Frozen Semen, Semen Quality, Simmental Bull

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