Percentage Destabilization Effect of Some West African Medicinal Plants on the Outer Membrane of Various Bacteria Involved in Infectious Diarrhea

Dougnon, Victorien; Hounsa, Edna; Agbodjento, Eric; Keilah, Lunga Paul; Lègba, Boris; Sintondji, Kévin; Afaton, Anny; Klotoé, Jean Robert; Baba-Moussa, Lamine; Bankolè, Honoré

doi:10.1155/2021/4134713

Cited by 5 publications

(4 citation statements)

References 24 publications

(28 reference statements)

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“…By contrast, MIC/2 and MIC/4 did not inhibit the bacterial growth as evidenced by the increase trend of the curves at these concentrations. These results are consistent with the antibiotic power (Pa=MBC/MIC: 25/12.5=2) obtained for both compounds 4a and 4b, which classify them as bactericidal according to the criteria reported by Dougnon et al [60] that elaborate bactericidal (1≤MBC/MIC≤2) and bacteriostatic (4≤MBC/MIC≤16) effects of potential antimicrobial compounds based on the ratio MBC/MIC. A paper by Kohanski et al in the September 7 issue of Cell [61] presented evidence that bactericidal antibiotics kill both Gram-and Gram+ bacteria by an as yet unappreciated mode: through overproduction of the destructive oxygen reduction product, the hydroxyl radical (HO•) [61].…”

Section: Discussionsupporting

confidence: 91%

Repurposing of Synthetic Acetaminophen Derivatives Containing Benzothiazole Scaffold as Alternative Therapy for Infectious Diarrhea Caused by Drug-Resistant Shigella Species

Pone Kamdem,

Pinlap,

Noumboue Kouamou

et al. 2024

Preprint

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Diarrhea remains one of the leading causes of mortality worldwide, especially among children. Recent epidemiological studies conducted in developing countries identified Shigella species as the most predominant pathogenic bacteria responsible for diarrhea. Antimicrobial therapy is necessary for Shigella infections; however, the rapid emergence of resistance against existing antimicrobials in Shigella spp. poses a serious global health problem. To identify alternative antimicrobial compounds with activity against Shigella species, the pathogens responsible for bacterial diarrhea. In this study, we have applied antibacterial phenotypic screening to identify potent anti-Shigella compounds across a broad chemical diversity, including selected acetaminophen derivatives containing benzothiazoles backbone, and their combination with certain antibiotics. In continuation to our effort in searching potent antimicrobial compounds, we have found this time around that two acetaminophen derivatives containing benzothiazoles backbone (4a and 4b) could inhibit the growth of Shigella flexneri with common MIC value of 12.5 µg/ml. These compounds were established through a time-kill kinetics’ study to be bactericidal. Meanwhile, the 2-aminobenzothiazoles (1a and 1b) used for the synthesis of compounds 4 (a & b) were found to be poorly active (MIC: 100 µg/ml) against this pathogen. Combination studies of 4a and 4b with the least susceptible antibiotics (ceftriaxone and cotrimoxazole) demonstrated synergistic anti-Shigella activity. The present study demonstrates that the azobenzothiazole dyes 4 (a & b) can be repurposed as potential anti-Shigella compounds, which can serve as scaffolds for the development of new agents against infectious diarrhea caused by Shigella and other enteric pathogens, especially in developing countries.

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Section: Discussionsupporting

confidence: 91%

Repurposing of Synthetic Acetaminophen Derivatives Containing Benzothiazole Scaffold as Alternative Therapy for Infectious Diarrhea Caused by Drug-Resistant Shigella Species

Pone Kamdem,

Pinlap,

Noumboue Kouamou

et al. 2024

Preprint

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“…[1]. The methanolic extract of the D. abyssinica root is active against this microorganism [94]; how-ever, in another study, it was only moderately active against the same microorganism [95]. Another medicinal plant, D. mespiliformis, is more active against C. neoformans than against C. albicans.…”

Section: Antifungal Activitymentioning

confidence: 99%

The Genus Diospyros: A Review of Novel Insights into the Biological Activity and Species of Mozambican Flora

Ribeiro,

Serrano,

da Silva

et al. 2023

Plants

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Species of the Diospyros L. genus (Ebenaceae family) have been largely used in traditional medicine for the treatment of several diseases, especially infectious ones. To date, active major compounds such as naphthoquinones, triterpenoids, and tannins have been isolated and pharmacologically validated from Diospyros species. The present study summarizes the information available in the literature on the species described in the Flora of Mozambique. To do so, scientific databases (e.g., PubMed, Scopus, Web of Science, and Google Scholar) were searched using various keywords and Boolean connectors to gather and summarize the information. Of the 31 native and naturalized species in the Flora of Mozambique, 17 are used in different regions of Africa and were described for their traditional uses. They were reported to treat more than 20 diseases, mostly infectious, in the gastrointestinal and oral cavity compartments. This work provides an overview of the therapeutical potential of Diospyros species and explores novel insights on the antimicrobial potential of extracts and/or isolated compounds of these Mozambican species.

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“…The extracts thus produced were placed in a refrigerator at 4°C and put back into solution during the different tests. Only extracts known for their proven antibacterial efficacy on strains involved in diarrheal infections were used in this study (12).…”

Section: Preparation Of Extractsmentioning

confidence: 99%

“…The treatment of diarrheal diseases in Benin relies in some communities on the use of certain medicinal plants of the Beninese flora, such as Khaya senegalensis, Daniellia oliveri, Vernonia amygdalina, Manihot esculenta, Ocimum gratissimum, Diospyros mespiliformis, Pterocarpus erinaceus, and Anacardium occidentale (11). Our previous studies on these plants have revealed their antibacterial effect on bacterial strains isolated from diarrheic stools (12). Another study on these plants further showed that they were composed of tannins (11).…”

Section: Introductionmentioning

confidence: 99%

Fetotoxicity and Subacute Toxicity of Some Plants Involved in the Treatment of Infectious Diarrhea in Benin

Hounsa¹,

Dougnon²,

Agbankpé³

et al. 2022

Front. Trop. Dis

Self Cite

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This study aimed to provide data on the subacute toxicity and fetotoxicity of aqueous and hydroethanolic extracts of Daniellia oliveri (Rolfe) Hutch. & Dalziel, Anacardium occidentale L., Diospyros mespiliformis Hochst. ex A. DC., Khaya senegalensis (Desv.) A. Juss., Ocimum gratissimum L., Vernonia amygdalina Delile, Pterocarpus erinaceus Poir., and Manihot esculenta Crantz used in the treatment of diarrheal diseases in Benin. For subacute toxicity, each male and female Wistar rat received by esophageal gavage a maximum of 1000 mg/kg over 28 days following the Organization for Economic Co-operation and Development (OECD) Guideline No. 407. Bodyweight changes, biochemical and hematological parameters were assessed. The fetotoxicity of the extracts was evaluated on Dutch Blue hen eggs at 100 mg/kg and 300 mg/kg. The hatching rate, biochemical and hematological constants were subsequently determined. The subacute toxicity data did not indicate any mortality or signs of toxicity. Also, no significant difference in the bodyweight of the rats and the hematological parameters was noted. Concerning fetotoxicity, a low hatching rate (varying from 20% to 50%) was observed in the batches treated with the extracts at 300mg/kg compared to the rate obtained in the negative control batch (80%). The batches that received the hydroethanolic extracts of Vernonia amygdalina and Manihot esculenta at the tested doses had a hatching rate of 0%. When looking at hematological parameters, no significant difference was noted for all batches. However, an increase in the Aspartate Amino-Transferase (ASAT) values was noted in the batches that received the hydroethanolic extract of Khaya senegalensis and the aqueous extract of Diospyros mespiliformis at 100 and 300 mg/kg. These results explicitly show that the hydroethanolic extracts of Manihot esculenta and Vernonia amygdalina are fetotoxic at the tested doses.

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Percentage Destabilization Effect of Some West African Medicinal Plants on the Outer Membrane of Various Bacteria Involved in Infectious Diarrhea

Cited by 5 publications

References 24 publications

Repurposing of Synthetic Acetaminophen Derivatives Containing Benzothiazole Scaffold as Alternative Therapy for Infectious Diarrhea Caused by Drug-Resistant Shigella Species

Repurposing of Synthetic Acetaminophen Derivatives Containing Benzothiazole Scaffold as Alternative Therapy for Infectious Diarrhea Caused by Drug-Resistant Shigella Species

The Genus Diospyros: A Review of Novel Insights into the Biological Activity and Species of Mozambican Flora

Fetotoxicity and Subacute Toxicity of Some Plants Involved in the Treatment of Infectious Diarrhea in Benin

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