Perbedaan Angiogenesis Pada Pulpa Setelah Aplikasi Ekstrak Propolis Dan Kalsium Hidroksida

Abstract

<jats:p>Background : Propolis is a resinous hive product collected by bees ( Apis Mellifera ) from tree buds and mixed with secreted bee wax in order to avoid bacterial contamination in the hive, and also to seal it. Propolis is employed for the treatment of various infectious diseases because it is wellknown that is has antibacterial and anti-inflammatory properties. Calcium hydroxide was introduced to the dental profession in 1921 and has been considered the “gold standard” for direct pulp capping materials in the past decades. Aims :This research is to investigate the development of new blood vessels ( angiogenesis ) in rat's dental pulp following application of propolis extract and calcium hydroxide. Methods : There was 43 Strain Wistar rats of 8–16 week old and 200–250 grams in weight were used in this study. The rats were randomly divided into 6 groups. Pulp exposures were performed on the occlusal surface of right maxillary first molars. At the 1st and 4th groups, as the control group, without pulp capping paste. At the 2rd and 5th groups, pulp exposure was applied with propolis extract. And at 3th and 6th groups pulp exposure was applied with calcium hydroxide ,and the 7th group is negative control is a normal teeth. Pulp exposure was applied with propolis extract. After that, all of the cavities were filled with light cured glass ionomer cement as a permanent filling. Animals on the 1st, 2rd, and 3th groups were decaputed after 7x24 hour post pulp capping material application and were sent for histological examination which new blood vessels ( angiogenesis ) cells were present. And at the 4th, 5th, 6th groups were culled after 7x 24 hour post pulp capping material application and were sent for histological examination which new blood vessels ( angiogenesis ) evaluated were present. Result : All sample were histopathological examinated and data was statiscally analysed using one way ANOVA the histological analysis revealed that the development of the new blood vessels occurred in all group. The new blood vessels ( angiogenesis ) of propolis extract group was milder compared to the control and calcium hydroxide group, with statistical analysis showed significant difference (p &gt; 0.05). Conclusion: The development of new blood vessels is earlier happened in group capping material containing propolis and which show with reduce the amount of the new blood vessels in days 7 and 14 than the other group.</jats:p>

Potential Combination of Acemannan Sponge and Calcium Phosphate Cement-Calcium Sulfate Hemihydrate (CPC-CSH) as Direct Pulp Capping Alternative Materials for Reparative Dentin Formation

“…Meanwhile, the pulp is a tooth structure that includes soft connective tissue in the blood vessels and nerves in the dental cavity which is located under the dentin. 22 Dentin chemically has a protein matrix composition and pathways that regulate the differentiation of odontoblasts and osteoblasts. Dentine morphologically does not have the ability to undergo remodeling, whereas dental pulp can differentiate into odontoblasts and osteoblasts, consisting of fibroblasts, endothelial cells, and progenitor cells.…”

<p>Potensi kombinasi scaffold gipsum puger dan aloe vera terhadap angiogenesis pada soket pasca ekstraksi gigi tikus Wistar jantan</p><p>The potential of the combination of hydroxyapatite gypsum puger and aloe vera scaffold on angiogenesis of the post-extraction socket of male Wistar rats<p>

“…Sedangkan, hari ke-14 digunakan dalam penelitian ini karena proses angiogenesis akan mengalami penurunan pada hari ke-14 karena pembuluh darah baru ini telah mengalami inhibisi dan remodeling. 19 Hasil penelitian yang telah dilakukan oleh peneiliti didapatkan rerata jumlah pembuluh darah tertinggi terdapat pada kelompok scaffold kombinasi HAGP dan AV atau kelompok PIII, sedangkan rerata jumlah pembuluh darah terendah terdapat pada kelompok kontrol. Setelah dilakukan uji LSD didapatkan hasil nahwa mayoritas mengalami perbedaan yang signifikan.…”

“…Hal ini karena proses penambahan jumlah pembuluh darah (angiogenesis) dimulai pada hari ke-5, dimana proses ini dimulai dengan migrasi sel-sel endotel menuju area traumatis yang diinduksi oleh faktorfaktor pro angiogenik, sedangkan pada hari ke-3 diduga tidak optimalnya faktor-faktor proangiogenik untuk menjamin proses pembentukan pembuluh darah . 18,19 Selain itu pada kelompok perlakuan hari ke -14, menunjukkan rerata jumlah meningkat dengan hasil uji LSD yang signifikan. Hal ini tidak sesuai dengan teori dimana seharusnya jumlah pembuluh darah pada hari ke-14 cenderung stabil atau bahkan menurun dikarenakan pembuluh darah baru telah mengalami inhibisi dan remodeling.…”

“…Proses inhibisi memiliki tujuan guna mencegah meningkatnya pembuluh darah yang berakibat pada pertumbuhan jaringan parut yang berlebih, sedangkan pada proses remodeling memiliki tujuan untuk mempermudah aliran darah ke jaringan baru. 19 Variabel yang diteliti dalam penelitian ini adalah angiogenesis, ini dilakukan dengan cara menghitung jumlah pembuluh darah dari setiap kelompok. Pembuluh darah mengalami peningkatan yang puncaknya berada pada hari ke-7.…”