Peran Thidiazuron Dalam Peningkatan Kemampuan Proliferasi Tanaman Secarain Vitro

Lestari, Endang Gati

doi:10.21082/jp3.v34n2.2015.p87-93

Cited by 4 publications

(5 citation statements)

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“…Artinya ada perbandingan perlakuan lainnya, TDZ pada konsentrasi rendah mampu menghasilkan tunas paling banyak. Thidiazuron berbeda dengan zat pengatur tumbuh lainnya, ia bekerja paling baik jika dalam konsentrasi rendah, namun jika konsentrasi tinggi dapat merusak jaringan dan mempersulit benih berkecambah (Lestari, 2015). Namun, dalam penelitian ini perlakuan TDZ 0,75 ppm (R7) menghasilkan tunas normal paling rendah dibandingkan dengan obat lain, artinya perlakuan kontrol (R0) dan perlakuan TDZ 1 ppm menghasilkan jumlah tunas lebih tinggi.…”

Section: Jumlah Tunas Per Eksplan (Buah)unclassified

Response of Shoot Growth of Pterocarpus indicus willd. to The Addition of BAP and TDZ In Vitro

Nofitria,

Violita,

Novita

et al. 2024

JBT

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Pterocarpus indicus Willd. also known as redwood is a native Philippine species belonging to the Fabaceae family that has many uses and high economic value and highly exploited. However, it is not accompanied by good regeneration which causes this species to be categorized as endangered (IUCN v. 2021-1). The in vitro technique is an alternative to overcome this problem. This study aims to determine the response of P. indicus Willd. shoot growth to the addition of BAP and TDZ in vitro. The research was conducted from August to December 2023, at the Biotechnology Laboratory, KST BJ. Habibie Puspiptek Serpong, South Tangerang, Banten. This study used a completely randomized design (CRD) consisting of 9 treatments and 14 replications. The treatments consisted of: R0 = Control, R1 = BAP 0,25 ppm, R2 = BAP 0,5 ppm, R3 = BAP 0,75 ppm, R4 = BAP 1 ppm, R5 = TDZ 0,25 ppm, R6 = TDZ 0,5 ppm, R7 = TDZ 0,75 ppm, R8 = TDZ 1 ppm. The results showed that the concentration of BAP 0,5 ppm gave the best results in height of shoot and TDZ 0,25 ppm gave optimum results in the number of shoots compared to the control and other treatment concentrations.

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Section: Jumlah Tunas Per Eksplan (Buah)unclassified

Response of Shoot Growth of Pterocarpus indicus willd. to The Addition of BAP and TDZ In Vitro

Nofitria,

Violita,

Novita

et al. 2024

JBT

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“…TDZ can be given together with other growth regulators, such as cytokinins or auxins. At the initiation of mangosteen shoots, using BA plus TDZ is the best treatment for shoot formation (Lestari et al 2015). TDZ stimulates shoot multiplication in several plants, including breadfruit (Supriati et al 2005) and aromatic ginger, by adding 0.1 mg/L TDZ to MS + BA 5 mg/L (Lestari & Hutami 2005).…”

Section: Shoot Regeneration After Conservationmentioning

confidence: 99%

“…PGR, such as BA has a significant role in cell division during plant metabolism in shoot induction and multiplication (Ashraf et al 2014). The exact concentration of BA for each plant is not the same depending on the type of plant, physiological conditions of the explant, type of primary media, environmental conditions of growth, and genetic factors (Lestari 2015). Propagation of ginger using ZPT 4.5 mg/L BA is the best concentration to stimulate shoot multiplication (Abbas et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Besides PGR, minerals are essential elements in media culture (Kumar & Reddy 2011). TDZ is a compound belonging to diphenyl urea and has almost the same activity as cytokinins, often used to stimulate cell division in shoot proliferation, especially in woody plants (Lestari 2015). Using a combination of TDZ with BA has succeeded in increasing the ability of cell proliferation in various plants, for example, Plumbago zeylanica (Syahid & Kristina 2008;Lestari et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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The Formula media <i>in vitro</i> Propagation and Conservation of <i>Ludwigia</i> sp.

Lestari¹,

Nugraha²,

Yunita³

2023

J.Tropical Biodiversity Biotechnology

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The aquatic plant "Red Malang” (Ludwigia sp.) has a fairly high economic value as an ornamental aquatic plant, so it has the potential to be developed. The growth of in vitro cultures in culture bottles is high-speed, so it is necessary to find a formula media to inhibit growth so that the frequency of subcultures is reduced. The current research aims to produce a formula media for shoot multiplication and in vitro culture conservation. The research was carried out at the ICABIOGRAD tissue culture laboratory from April 2020 to June 2021. Research activities included plant propagation, conservation, and regeneration after conservation. Plant material was using in the form of a culture collection in the ICABIOGRAD tissue culture laboratory, treatment media for propagation were BA (0; 0.1; 0.3; 0.5; 0.7 and 0.9 mg/L) + thidiazuron (TDZ) (0 and 0.1mg/L). For conservation were MS + BA medium (0 and 0.1 mg/L) + paclobutrazol (0; 0.1; 0.3; 0.5; 0.7 mg/L) and for shoot regeneration after conservation using MS medium without Plant Growth Regulator (PGR). Data analysis using the Anova SAS version 9.0 test program. Further test using DMRT test with alpha level 5%. There was no difference in the mean value between levels of TDZ treatment on the number of shoots and leaves. The difference in the mean value between levels of TDZ treatment was very significant on shoot height, the number of roots, and root length. BA treatment with a concentration of 0.7 mg/L is better because it gives higher results for each observation variable. For conservation, treatment with paclobutrazol 0.5 mg/L inhibited shoot and leaf count, and 0.3 mg/L inhibited shoot formation. Cultures stored for six months grew normally after being regenerated. The highest shoots and the highest number of leaves were obtained from the treatment of paclobutrazol without BA. This study indicated that the propagation media of aquatic plants Ludwigia sp. did not require high concentrations of BA. Cultures could be stored for over six months using paclobutrazol with 0.3-0.6 mg/L.

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“…TDZ merupakan hormon yang dapat menginduksi embrio somatik pada tanaman berkayu dengan konsentrasi lebih dari 1 µM atau 0,22 ppm, karena memiliki aktivitas yang mirip seperti sitokinin, yaitu melakukan pembelahan sel dan organogenesis (Huetteman dan Preece, 1993). Menurut Lestari (2015), konsentrasi TDZ untuk menginduksi embrio somatik dapat dilakukan menggunakan konsentrasi yang rendah, yaitu sekitar 0,1 -0,5 mg/l. Terjadinya abnormalitas dan terhambatnya pertumbuhan embrio somatik dapat disebabkan oleh konsentrasi TDZ yang terlalu tinggi, karena TDZ merupakan herbisida yang dapat membunuh jaringan pada eksplan dan menyebabkan penyimpangan pada perkembangan jaringan tanaman (Ouyang et al, 2016).…”

Section: Pengaruh Hormon Terhadap Embriogenesis Somatikunclassified

Pengaruh Hormon terhadap Induksi Embrio Somatik Kacapiring (Gardenia jasminoides) dan Potensi Aplikasinya dalam Pembuatan Benih Sintetik

Pardede

Sidharta

2021

Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati

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Kacapiring (Gardenia jasminoides) is an ornamental plant that has many uses, as a cut flowers, food ingredients, textile materials, cosmetic raw materials, and containing therapeutic compounds. The high demand for gardenia needs to be balanced with the results of its cultivation. Conventional propagation of gardenias has disadvantages such as a long life cycle, genotypes can differ from the parent, and can carry disease from the parent. Therefore, more efficient gardenia propagation techniques are needed, one of which is by making synthetic seeds. Synthetic seeds can be produced by encapsulating somatic embryos. Somatic embryos are collections of embryonic cells formed from soma cells that have the ability to regenerate into a complete new plant. Hormones are organic materials that occur naturally in plants or are synthetic. Hormones have an effect on plant differentiation and growth. Somatic embryogenesis can be carried out using auxin and cytokinin hormones, because auxins can induce embryogenic callus and cytokinins can initiate the embryogenic process in callus. Synthetic seeds are single somatic embryos covered by a matrix, so that they can be stored for a certain duration of time and can grow into a complete plant in both in vitro and ex vitro conditions. This literature review is to provide an overview based on existing references on the effects of various types of hormones for the induction of gardenia somatic embryos, synthetic seed production methods, and synthetic seed storage temperatures.

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Peran Thidiazuron Dalam Peningkatan Kemampuan Proliferasi Tanaman Secarain Vitro

Cited by 4 publications

References 0 publications

Response of Shoot Growth of Pterocarpus indicus willd. to The Addition of BAP and TDZ In Vitro

Response of Shoot Growth of Pterocarpus indicus willd. to The Addition of BAP and TDZ In Vitro

The Formula media <i>in vitro</i> Propagation and Conservation of <i>Ludwigia</i> sp.

Pengaruh Hormon terhadap Induksi Embrio Somatik Kacapiring (Gardenia jasminoides) dan Potensi Aplikasinya dalam Pembuatan Benih Sintetik

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