PepYLCIV and PepYLCAV resistance gene Pepy-2 encodes DFDGD-Class RNA-dependent RNA polymerase in Capsicum

Koeda, Sota; Mori, Namiko; Horiuchi, Ryo; Watanabe, Chiho; Nagano, Atsushi J.; Shiragane, Hayato

doi:10.1007/s00122-022-04125-9

Cited by 14 publications

(12 citation statements)

References 61 publications

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“…Over the past three decades, epidemics of diseases caused by begomoviruses have emerged to be an important constraint to the production of solanaceous crops in many tropical and subtropical regions of the world (Kenyon et al 2014). Genetic resistance to begomoviruses is an ideal means of controlling the diseases, and the isolation of several resistance genes from tomato and pepper has accelerated markerassisted breeding; the genes Ty-1/Ty-3, Ty-2, and ty-5 provide resistance against tomato yellow leaf curl virus, and pepy-1 and Pepy-2 provide resistance against PepYLCIV and PepYLCAV (Verlaan et al 2013;Lapidot et al 2015;Yamaguchi et al 2018;Koeda et al 2021Koeda et al , 2022. However, breeding for resistance to begomoviruses is much less advanced in eggplant compared with that in tomato and pepper.…”

Section: Discussionmentioning

confidence: 99%

“…To analyze the resistance of these candidate accessions in further detail, we conducted graft-inoculation of TYLCKaV. Graft-inoculation of a begomovirus is a reliable method to deliver a begomovirus to plants without inoculation escapes (Anaya-López et al 2003;Vanderschuren et al 2012;Koeda et al 2018Koeda et al , 2021Koeda et al , 2022Mori et al 2022). From the graft-inoculation experiment, 23 accessions were classi ed as highly resistant and 10 accessions as moderately resistant to TYLCKaV (Table 3).…”

Section: Discussionmentioning

confidence: 99%

“…However, chemical control has been only partially effective because most crops in tropical and subtropical regions are grown in open elds. This has led to strong demand for genetic resistance to begomoviruses for improved control of diseases, and the cloning of resistance genes in tomato and pepper has enabled marker-assisted breeding of resistant cultivars (Verlaan et al 2013;Lapidot et al 2015;Yamaguchi et al 2018;Koeda et al 2021Koeda et al , 2022. However, breeding for begomovirus resistance is much less advanced in eggplant compared with that in tomato and pepper.…”

Section: Introductionmentioning

confidence: 99%

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Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena

et al. 2023

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Yellow leaf curl disease caused by begomoviruses has emerged as a major limitation to the production of eggplant (Solanum melongena) in several regions of the world. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) is a bipartite begomovirus isolated from diseased eggplant in the areas of the Indochinese peninsula, South China, and Maritime Southeast Asia. In this study, ve begomovirus species isolated in Indonesia, including TYLCKaV, were inoculated to eggplant using infectious clones. Among the inoculated begomoviruses, TYLCKaV alone induced typical yellowing and curling symptoms in eggplant.Inoculation of TYLCKaV to a panel of crop species (eggplant, tomato, pepper, tobacco, cucumber, melon, and squash) commonly grown in Indonesia clari ed that TYLCKaV infects and causes yellow leaf curl disease mainly in eggplant and tomato. Agroin ltration of TYLCKaV to 736 accessions of eggplant enabled selection of 33 resistant candidates. Further evaluation by TYLCKaV graft-inoculation of the resistant candidates clari ed that 10 accessions were classi able as moderately resistant and 23 accessions as highly resistant. Among the highly resistant accessions, 22 accessions restricted viral DNA accumulation to a signi cantly lower amount than the susceptible control. These 22 highly resistant accessions represent valuable genetic resources for breeding begomovirus resistance in eggplant.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena

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“…In 2017, pepper yellow leaf curl Aceh virus (PepYLCAV) was isolated as a novel recombinant begomovirus species infecting pepper, tomato, and tobacco (Nicotiana tabacum) plants exhibiting leaf yellowing and curling symptoms in Aceh Province, which is located on the northern end of Sumatra (Kesumawati et al 2019). Interestingly, compared with the effects of its putative major parent (PepYLCIV), PepYLCAV was revealed to induce more severe symptoms on pepper plants, while also signi cantly inhibiting growth (Koeda et al 2021(Koeda et al , 2022. Substantial damages and production losses caused by begomoviral infections necessitated the breeding of begomovirus-resistant cultivars (Rojas et al 2018), with the most notable advances in the breeding for begomovirus resistance reported for tomato.…”

Section: Introductionmentioning

confidence: 99%

A novel recombinant begomovirus isolated in Southeast Asia breaks the Ty-1-mediated resistance in tomato

Pohan,

Kanae,

Okabe

et al. 2024

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Infections by begomoviruses have threatened the global production of multiple crops, including Solanaceae species. Recombination, a phenomenon frequently observed in begomoviruses, has profound effects on virus populations, enabling them to adapt to changing environmental conditions. We previously isolated pepper yellow leaf curl Aceh virus (PepYLCAV) from pepper (Capsicum annuum), tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum) plants in Aceh Province, Indonesia and determined it is a recombinant of the widely distributed pepper yellow leaf curl Indonesia virus (PepYLCIV). To compare PepYLCAV with its major putative parent PepYLCIV in terms of virulence, multiple solanaceous plants (Nicotiana benthamiana, pepper, and tomato) were inoculated with the two viruses. Disease symptom severity, growth inhibition, and viral DNA accumulation were greater for the infection with PepYLCAV than for the infection with PepYLCIV. Furthermore, the inoculation with PepYLCAV revealed the Ty-1-mediated resistance of the begomovirus-resistant tomato cultivar was compromised, resulting in moderate symptoms, which were not detected in the plants infected with PepYLCIV. According to the polymerase chain reaction-based re-analysis of samples collected from fields in Indonesia from 2012 to 2019, PepYLCAV rather than PepYLCIV has been the predominant species since 2017. In conclusion, PepYLCAV, which is a novel recombinant bipartite begomovirus that is more virulent and widespread than its major putative parent (PepYLCIV), can overcome the Ty-1-mediated resistance of commercial F1 hybrid tomato cultivars.

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“…An integrated pest management approach which uses begomovirus-resistant cultivars can be an effective alternative to control begomoviruses. Several begomovirus resistance genes have been cloned from Solanaceae crops using elaborate forward and reverse genetic methods [32][33][34][35][36]. Among the identi ed resistance genes, Ty-1/Ty-3 in tomato and Pepy-2 in capsicum encode DFDGD-class RNA-dependent RNA polymerases (RDRs) and are dominant resistance genes to multiple begomoviruses [33,35].…”

Section: Introductionmentioning

confidence: 99%

Cy-1, a major QTL for tomato leaf curl New Delhi virus resistance, harbors a gene encoding a DFDGD-Class RNA-dependent RNA polymerase in cucumber (Cucumis sativus)

Koeda,

Yamamoto,

Yamamoto

et al. 2023

Preprint

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Background Tomato leaf curl New Delhi virus (ToLCNDV) (family Geminiviridae, genus Begomovirus) is a significant threat to cucumber (Cucumis sativus) production in many regions. Previous studies have reported the genetic mapping of loci related to ToLCNDV resistance, but no resistance genes have been identified. Results We conducted map-based cloning of the ToLCNDV resistance gene in cucumber accession No.44. Agroinfiltration and graft-inoculation analyses confirmed the resistance of No.44 to ToLCNDV isolates from the Mediterranean and Asian countries. Initial mapping involving two rounds of phenotyping with two independent F2 populations generated by crossing the begomovirus-susceptible cultivar SHF and No.44 consistently detected major quantitative trait loci (QTLs) on chromosomes 1 and 2 that confer resistance to ToLCNDV. Fine-mapping of Cy-1, the dominant QTL on chromosome 1, using F3 populations narrowed the candidate region to a 209-kb genomic segment harboring 24 predicted genes. Among these genes, DFDGD-class RNA-dependent RNA polymerase (CsRDR3), an ortholog of Ty-1/Ty-3 of tomato and Pepy-2 of capsicum, was found to be a strong candidate conferring ToLCNDV resistance. The CsRDR3 sequence of No.44 contained multiple amino acid substitutions; the promoter region of CsRDR3 in No.44 had a large deletion; and the CsRDR3 transcript levels were greater in No.44 than in SHF. Virus-induced gene silencing (VIGS) of CsRDR3 using two chromosome segment substitution lines harboring chromosome 1 segments derived from No.44 compromised resistance to ToLCNDV. Conclusions Forward and reverse genetic approaches identified CsRDR3, which encodes a DFDGD-class RNA-dependent RNA polymerase, as the gene responsible for ToLCNDV resistance at the major QTL Cy-1 on chromosome 1 in cucumber. Marker-assisted breeding of ToLCNDV resistance in cucumber will be expedited by using No.44 and the DNA markers developed in this study.

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PepYLCIV and PepYLCAV resistance gene Pepy-2 encodes DFDGD-Class RNA-dependent RNA polymerase in Capsicum

Cited by 14 publications

References 61 publications

Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena

Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena

A novel recombinant begomovirus isolated in Southeast Asia breaks the Ty-1-mediated resistance in tomato

Cy-1, a major QTL for tomato leaf curl New Delhi virus resistance, harbors a gene encoding a DFDGD-Class RNA-dependent RNA polymerase in cucumber (Cucumis sativus)

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