Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena

Abstract

Yellow leaf curl disease caused by begomoviruses has emerged as a major limitation to the production of eggplant (Solanum melongena) in several regions of the world. Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) is a bipartite begomovirus isolated from diseased eggplant in the areas of the Indochinese peninsula, South China, and Maritime Southeast Asia. In this study, ve begomovirus species isolated in Indonesia, including TYLCKaV, were inoculated to eggplant using infectious clones. Among the inoculated begomoviruses, TYLCKaV alone induced typical yellowing and curling symptoms in eggplant.Inoculation of TYLCKaV to a panel of crop species (eggplant, tomato, pepper, tobacco, cucumber, melon, and squash) commonly grown in Indonesia clari ed that TYLCKaV infects and causes yellow leaf curl disease mainly in eggplant and tomato. Agroin ltration of TYLCKaV to 736 accessions of eggplant enabled selection of 33 resistant candidates. Further evaluation by TYLCKaV graft-inoculation of the resistant candidates clari ed that 10 accessions were classi able as moderately resistant and 23 accessions as highly resistant. Among the highly resistant accessions, 22 accessions restricted viral DNA accumulation to a signi cantly lower amount than the susceptible control. These 22 highly resistant accessions represent valuable genetic resources for breeding begomovirus resistance in eggplant.

Begomovirus Infection Patterns in Melons of Indonesian Lowlands: Growth Trends Among Ecosystems and Genotypes

“…Because of this global distribution of its vectors, begomovirus has become a major threat to vegetable crops in tropical and semitropical regions (Lapidot and Friedmann 2002;Mansoor et al 2003;Rojas et al 2005;Seal et al 2006;Varma and Malathi 2003). Especially for melon, several begomovirus species that has been reported to infect this plant are Tomato yellow leaf curl Kanchanaburi virus (Kikkawa et al 2023), Tomato leaf curl Palampur virus (Shahid 2023), Tomato leaf curl New Delhi virus (Chang et al 2023), and Cotton leaf curl Gezira virus (Gambley et al 2020).…”