Peptoniphilus mikwangii sp. nov., Isolated from a Clinical Specimen of Human Origin

Abstract

A novel Gram-positive, anaerobic, and coccus-shaped bacterium, designated as strain ChDC B134(T), was isolated from a human postoperative infectious lesion in the right maxilla between the lateral incisor and canine and was characterized by polyphasic taxonomic analysis. 16S rRNA gene sequence analysis revealed that the strain ChDC B134(T) belonged to the genus Peptoniphilus, as it showed sequence similarities to Peptoniphilus indolicus KCTC 15023(T) (94.0 %) and Peptoniphilus asaccharolyticus KCTC 3321(T) (93.8 %). The prevalent fatty acids of of strain ChDC B134(T) were C16:0 (20.3 %), C18:1 cis 9 (34.3 %), and C18:0 (13.2 %). The DNA G+C content was 30.9 mol%. The cell wall diamino acid was D-ornithine, which is a property shared by other reference type strains of the genus Peptoniphilus. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC B134(T) (=KCOM 1628(T) = KCTC 15227(T) = JCM 30223(T)) should be classified as the type strain of a novel species of genus Peptoniphilus, for which the name Peptoniphilus mikwangii sp. nov. is proposed.

Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse

“…(). C 16 : 0 , one of the major fatty acids of CBA3646 T , has also been shown to be the major fatty acid of strains of members of the genus Peptoniphilus [13–18], indicating that CBA3646 T shares cellular properties with members of the genus Peptoniphilus . C 14 : 0 was a major fatty acid in P.…”

“…The phenotypic characteristics of CBA3646 T and those of the reference strains P. asaccharolyticus KCTC 3321 3). C 16 : 0 , one of the major fatty acids of CBA3646 T , has also been shown to be the major fatty acid of strains of members of the genus Peptoniphilus [13][14][15][16][17][18], indicating that CBA3646 T shares cellular properties with members of the genus Peptoniphilus. C 14 : 0 was a major fatty acid in P. asaccharolyticus KCTC 3321 T but was a minor fatty acid in CBA3646 T .…”

“…its species were formerly classified as members of the genus Peptostreptococcus) in 2001 on the basis of the sequence of the 16S rRNA gene [8,12]. Species of the genus Peptoniphilus have been successfully isolated from various human clinical specimens [13][14][15][16][17] and swine faeces [18,19]. Bacteria of the genus Peptoniphilus are Gram-stain-positive, non-motile, anaerobic cocci.…”