Peptidomic and proteomic analyses of the systemic immune response of Drosophila

Lévy, Francine; Rabel, David; Charlet, Maurice; Bulet, Philippe; Hoffmann, Jules A.; Ehret‐Sabatier, Laurence

doi:10.1016/j.biochi.2004.07.007

Cited by 113 publications

(135 citation statements)

References 57 publications

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“…A number of proteins with immune functions were shown to be increased in intensity at 24 h but declined thereafter (Table 1). The 26 kDa (spot 4) and 32 kDa ferritin (spot 5) proteins are important in maintaining the homeostasis of iron in the haemocoel and have important roles in the immune response (Levy et al, 2004). Both proteins show a 1.34-1.4-fold increase in intensity at 24 h but this declined at 48 and 72 h. Transferrin (spot 1) showed a 2.27-fold increase in expression at 24 h compared to the control, however the intensity declined to 1.62 and 1.25-fold at 48 and 72 h, respectively.…”

Section: Analysis Of Changes In Protein Expression In Stressed Larvaementioning

confidence: 99%

Thermal and physical stresses induce a short-term immune priming effect in Galleria mellonella larvae

Browne

Surlis

Kavanagh

2014

Journal of Insect Physiology

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a b s t r a c tExposure of larvae of Galleria mellonella larvae to mild physical (i.e. shaking) or thermal stress for 24 h increased their ability to survive infection with Aspergillus fumigatus conidia however larvae stressed in a similar manner but incubated for 72 h prior to infection showed no elevation in their resistance to infection with A. fumigatus. Stressed larvae demonstrated an elevated haemocyte density 24 h after initiation of the stress event but this declined at 48 and 72 h. Larval proteins such as apolipophorin, arylophorin and prophenoloxidase demonstrated elevated expression at 24 h but not at 72 h. Larvae maintained at 37°C showed increased expression of a range of antimicrobial and immune-related proteins at 24 h but these decreased in expression thereafter. The results presented here indicate that G. mellonella larvae are capable of altering their immune response following exposure to mild thermal or physical stress to mount a response capable of counteracting microbial infection which reaches a peak 24 h after the initiation of the priming event and then declines by 72 h. A short-term immune priming effect may serve to prevent infection but maintaining an immune priming effect for longer periods may be metabolically costly and unnecessary while living within the colony of another insect.

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Section: Analysis Of Changes In Protein Expression In Stressed Larvaementioning

confidence: 99%

Thermal and physical stresses induce a short-term immune priming effect in Galleria mellonella larvae

Browne

Surlis

Kavanagh

2014

Journal of Insect Physiology

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“…Recently, this approach was successfully used in studies of different aspects of entomology, such as induced immunity (Levy et al, 2004), resistance mechanisms (Jiang et al, 2004), molecular pathology (Kaeslin et al, 2005), developmental process (Li et al, 2006;Jia et al, 2007) and diapause (Joplin et al, 1990;Li et al, 2007). In this report, a proteomic approach was used to investigate the proteome of wheat blossom midge larvae in different stages of diapause in order to determine the relationship between protein expression and insect diapause.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of pre-diapause, diapause and post-diapause larvae of the wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)

Cheng¹,

Li²,

Yu³

et al. 2009

Eur. J. Entomol.

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Abstract. To determine the relationship between protein expression and insect diapause, a proteomic approach was used to investigate the proteins extracted from larvae of the wheat blossom midge Sitodiplosis mosellana Gehin at different developmental stages, including pre-diapause, over-summering diapause, over-wintering diapause and post-diapause. Using 2-DE gels stained with coomassie brilliant blue, about 300 protein spots were detected in the extracts of pre-diapause larvae and 275 for those in each of the other stages. There were 91, 92 and 95 protein spots that showed more than a 2-fold change in abundance in the over-summering diapause, over-wintering diapause and post-diapause stages compared with pre-diapause. Eight protein spots, which showed the greatest difference in the larvae at different stages of diapause, were analyzed using Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Seven of them were successfully identified from their peptide mass fingerprints using the NCBInr database. They were proopiomelanocortin, NADH dehydrogenase subunit 1 and F10F2.5, which were up-regulated or unique to pre-diapause larvae, IKK interacting protein isoform 2 up-regulated in diapause and post-diapause larvae, GA10647-PA unique in over-wintering diapause larvae, purple CG16784-PB isoform B and B0228.6 up-regulated in over-summering and overwintering diapause larvae. The potential functions of these proteins during wheat blossom midge diapause are discussed.

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“…Parasito-proteomics studies are in their infancy but have already led to new insights concerning molecular pathogenesis and microorganism identification (Biron et al, 2005b;Levy et al, 2004;Moura et al, 200;Moura et al, 2003;Vierstraete et al, 2004). However, many parasito-proteomics studies performed so far have employed powerful tools but lack a conceptual approach to disentangle the host and parasite genome responses during their interactions.…”

Section: Molecular Manipulative Strategiesmentioning

confidence: 99%

Host–parasite molecular cross-talk during the manipulative process of a host by its parasite

Biron

Loxdale

2013

Journal of Experimental Biology

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SummaryMany parasite taxa are able to alter a wide range of phenotypic traits of their hosts in ways that seem to improve the parasiteʼs chance of completing its life cycle. Host behavioural alterations are classically seen as compelling illustrations of the ʻextended phenotypeʼ concept, which suggests that parasite genes have phenotype effects on the host. The molecular mechanisms and the host-parasite cross-talk involved during the manipulative process of a host by its parasite are still poorly understood. In this Review, the current knowledge on proximate mechanisms related to the ʻparasite manipulation hypothesisʼ is presented. Parasite genome sequences do not themselves provide a full explanation of parasite biology nor of the molecular cross-talk involved in host-parasite associations. Recently, first-generation proteomics tools have been employed to unravel some aspects of the parasite manipulation process (i.e. proximate mechanisms and evolutionary convergence) using certain model arthropodhost-parasite associations. The pioneer proteomics results obtained on the manipulative process are here highlighted, along with the many gaps in our knowledge. Candidate genes and biochemical pathways potentially involved in the parasite manipulation are presented. Finally, taking into account the environmental factors, we suggest new avenues and approaches to further explore and understand the proximate mechanisms used by parasite species to alter phenotypic traits of their hosts.

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Peptidomic and proteomic analyses of the systemic immune response of Drosophila

Cited by 113 publications

References 57 publications

Thermal and physical stresses induce a short-term immune priming effect in Galleria mellonella larvae

Thermal and physical stresses induce a short-term immune priming effect in Galleria mellonella larvae

Proteomic analysis of pre-diapause, diapause and post-diapause larvae of the wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae)

Host–parasite molecular cross-talk during the manipulative process of a host by its parasite

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