Peptides with antilipolytic and lipogenic activities from seeds of the bitter gourd Momordica charantia (Family Cucurbitaceae)

Ng, Tzi Bun; Wong, Chun-Kwok; Li, W.W.; Yeung, H.W.

doi:10.1016/0306-3623(87)90011-5

Cited by 14 publications

(6 citation statements)

References 12 publications

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“…The extracts exhibited significant antimicrobial activity against both gram positive and gram negative bacterial and fungal pathogens as well as cytotoxicity against brine shrimp nauplii (Saikot et al, 2012 ). Chemical constituents obtained from leaves of M. charantia showed hypoglycemic effect and can promote the release of insulin (Ng et al, 1986 ; Hui et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India

et al. 2016

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Plants have been used since ancient times as an important source of biologically active substances. The aim of the present study was to investigate the phytochemical constituents (flavonoids and phenolics), antioxidant potential, cytotoxicity against HepG2 (human hepato carcinoma) cancer cell lines, and the antimicrobial activity of the methanol extract of selected traditional medicinal plants collected from Mizoram, India. A number of phenolic compounds were detected using HPLC-DAD-ESI-TOF-MS, mainly Luteolin, Kaempferol, Myricetin, Gallic Acid, Quercetin and Rutin, some of which have been described for the first time in the selected plants. The total phenolic and flavonoid contents showed high variation ranging from 4.44 to 181.91 μg of Gallic Acid equivalent per milligram DW (GAE/mg DW) and 3.17 to 102.2 μg of Quercetin/mg, respectively. The antioxidant capacity was determined by DPPH (IC50 values ranges from 34.22 to 131.4 μg/mL), ABTS (IC50 values ranges from 24.08 to 513.4 μg/mL), and reducing power assays. Antimicrobial activity was assayed against gram positive (Staphylococcus aureus), gram negative (Escherichia coli, Pseudomonas aeruginosa), and yeast (Candida albicans) demonstrating that the methanol extracts of some plants were efficacious antimicrobial agents. Additionally, cytotoxicity was assessed on human hepato carcinoma (HepG2) cancer cell lines and found that the extracts of Albizia lebbeck, Dillenia indica, and Bombax ceiba significantly decreased the cell viability at low concentrations with IC50 values of 24.03, 25.09, and 29.66 μg/mL, respectively. This is the first report of detection of phenolic compounds along with antimicrobial, antioxidant and cytotoxic potential of selected medicinal plants from India, which indicates that these plants might be valuable source for human and animal health.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India

et al. 2016

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“…M. charantia is widely used as a food and as a medicine. The seeds of M. charantia contain peptides having antilipolytic and lipogenic activities (Ng et al, 1987). The present study aimed to further assess the effect of M. charantia extract on lipid profiles and oral glucose tolerance in diabetic rats.…”

Section: Introductionmentioning

confidence: 99%

Effect of Momordica charantia on lipid proﬁle and oral glucose tolerance in diabetic rats

Chaturvedi¹,

George²,

Milinganyo³

et al. 2004

Phytotherapy Research

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In this study, the methanol extract of Momordica charantia fruit extract was administered to diabetic rats to assess the long term effect of the extract on the lipid profile and the oral glucose tolerance test. Treatment for 30 days showed a significant decrease in triglyceride, low density lipoprotein and a significant increase in high density lipoprotein level. A significant effect on oral glucose tolerance was also noted. Chronic administration showed an improvement in the oral glucose tolerance curve. The effect was more pronounced when the test was done in rats fed the extract on the day of the test compared with tests done in rats which were not fed the extract on the same day.

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“…Yet they do not activate ribosomes or do so only weakly. However, only two seed lectins from this family [ Momordica charantia lectin (MCL), and Trichosanthes kirilowii seed lectin (TKL‐I)] were characterized in detail with respect to carbohydrate binding and macromolecular properties (12, 15, 17–25). To fill this lacuna, we took up a long‐term research program to investigate and characterize cucurbit seed lectins and reported the high yield purification and characterization of three galactose‐specific lectins from the seeds of Trichosanthes anguina , Trichosanthes cucumerina, and Trichosanthes dioica (26–39).…”

Section: Introductionmentioning

confidence: 99%

Purification and physicochemical characterization of two galactose‐specific isolectins from the seeds of Trichosanthes cordata

2009

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SummaryA galactose-specific lectin has been purified from the seeds of Trichosanthes cordata by affinity chromatography on crosslinked guar gum. The affinity-eluted lectin could be resolved into two isolectins, TCA-I and TCA-II by ion-exchange chromatography on DEAE cellulose. The molecular weights of the isolectins were determined as 59 and 52 kDa by SDS-PAGE. TCA-I is a heterodimer in which the two subunits with masses of 32 and 27 kDa, are covalently connected by disulfide bonds. TCA-I and TCA-II are glycoproteins with 6.2% and 6.8% covalently bound neutral sugar, respectively. CD spectroscopic studies indicate that the two isolectins are very similar in secondary structure and contain about 8 to 10% a-helix, 37-38% b-sheet, 20% b-turns, and 32-33% unordered structures. These isolectins have similar carbohydrate specificities as revealed by hemagglutination-inhibition assays. Carbohydrate specificity, subunit size and composition, and secondary structure of TCA isolectins suggest close similarity to type-II ribosome inactivating proteins. The agglutination activity of TCA-I was found to be highest in the pH range 7.0-8.0. The lectin activity was unaffected between 0 and 408C, but decreased dramatically above 408C. Association constant for the interaction of TCA-I with lactose was determined by monitoring ligand-induced changes in the protein intrinsic fluorescence characteristics as 7.42 3 10 3 M 21 at 258C. The exposure and accessibility of the tryptophan residues of TCA-I and the effect of ligand binding on them have been probed by quenching studies employing neutral and ionic quenchers.

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Peptides with antilipolytic and lipogenic activities from seeds of the bitter gourd Momordica charantia (Family Cucurbitaceae)

Cited by 14 publications

References 12 publications

Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India

Evaluation of Phenolic Content Variability along with Antioxidant, Antimicrobial, and Cytotoxic Potential of Selected Traditional Medicinal Plants from India

Effect of Momordica charantia on lipid proﬁle and oral glucose tolerance in diabetic rats

Purification and physicochemical characterization of two galactose‐specific isolectins from the seeds of Trichosanthes cordata

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