Peptides to combat viral infectious diseases

Al-Azzam, Shams; Ding, Yun; Liu, Jinsha; Pandya, Priyanka; Ting, Joey; Afshar, Sepideh

doi:10.1016/j.peptides.2020.170402

Cited by 44 publications

(26 citation statements)

References 210 publications

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“…Natural and synthetic antimicrobial peptides (AMPs), also called Host Defense Proteins/Peptides (HDPs), comprise potentially effective countermeasures against COVID-19, having shown inhibitory activity against multiple viruses [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. Integral components of the innate immune response, HDPs are typically small (12–80 amino acids) proteins and peptides expressed widely in the animal kingdom that serve as the “first line of defense” against foreign pathogens and potential subsequent infection and related inflammation.…”

Section: Introductionmentioning

confidence: 99%

Brilacidin Demonstrates Inhibition of SARS-CoV-2 in Cell Culture

Bakovic

Risner

Bhalla

et al. 2021

Viruses

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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the newly emergent causative agent of coronavirus disease-19 (COVID-19), has resulted in more than two million deaths worldwide since it was first detected in 2019. There is a critical global need for therapeutic intervention strategies that can be deployed to safely treat COVID-19 disease and reduce associated morbidity and mortality. Increasing evidence shows that both natural and synthetic antimicrobial peptides (AMPs), also referred to as Host Defense Proteins/Peptides (HDPs), can inhibit SARS-CoV-2, paving the way for the potential clinical use of these molecules as therapeutic options. In this manuscript, we describe the potent antiviral activity exerted by brilacidin—a de novo designed synthetic small molecule that captures the biological properties of HDPs—on SARS-CoV-2 in a human lung cell line (Calu-3) and a monkey cell line (Vero). These data suggest that SARS-CoV-2 inhibition in these cell culture models is likely to be a result of the impact of brilacidin on viral entry and its disruption of viral integrity. Brilacidin demonstrated synergistic antiviral activity when combined with remdesivir. Collectively, our data demonstrate that brilacidin exerts potent inhibition of SARS-CoV-2 against different strains of the virus in cell culture.

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Section: Introductionmentioning

confidence: 99%

Brilacidin Demonstrates Inhibition of SARS-CoV-2 in Cell Culture

Bakovic

Risner

Bhalla

et al. 2021

Viruses

View full text Add to dashboard Cite

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“…Peptide-based therapeutics have been widely studied and developed to treat viral infections [ 14 , 15 ]. Moreover, the use of bioactive peptides presents advantages including high specificity, efficacy, broad spectrum activity, and safety.…”

Section: Introductionmentioning

confidence: 99%

Non-Toxic Dimeric Peptides Derived from the Bothropstoxin-I Are Potent SARS-CoV-2 and Papain-like Protease Inhibitors

et al. 2021

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The COVID-19 outbreak has rapidly spread on a global scale, affecting the economy and public health systems throughout the world. In recent years, peptide-based therapeutics have been widely studied and developed to treat infectious diseases, including viral infections. Herein, the antiviral effects of the lysine linked dimer des-Cys11, Lys12,Lys13-(pBthTX-I)2K ((pBthTX-I)2K)) and derivatives against SARS-CoV-2 are reported. The lead peptide (pBthTX-I)2K and derivatives showed attractive inhibitory activities against SARS-CoV-2 (EC50 = 28–65 µM) and mostly low cytotoxic effect (CC50 > 100 µM). To shed light on the mechanism of action underlying the peptides’ antiviral activity, the Main Protease (Mpro) and Papain-Like protease (PLpro) inhibitory activities of the peptides were assessed. The synthetic peptides showed PLpro inhibition potencies (IC50s = 1.0–3.5 µM) and binding affinities (Kd = 0.9–7 µM) at the low micromolar range but poor inhibitory activity against Mpro (IC50 > 10 µM). The modeled binding mode of a representative peptide of the series indicated that the compound blocked the entry of the PLpro substrate toward the protease catalytic cleft. Our findings indicated that non-toxic dimeric peptides derived from the Bothropstoxin-I have attractive cellular and enzymatic inhibitory activities, thereby suggesting that they are promising prototypes for the discovery and development of new drugs against SARS-CoV-2 infection.

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“…The peptides used in therapy can block the viral attachment, the gene release especially in respiratory disease, and the viral protein assembly in SARS and COVID-19. The development of peptide-based vaccines aims to achieve the activation of B and/or T cells to promote an immune response [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…Even though the outbreak started at the end of 2019, the structure of the virus was extensively studied based on previous research on SARS-CoV-1 virus, which was responsible for the SARS epidemic that affected more than 8000 patients and had 10% mortality. It was proved that the second version has 79% genetic similarities but uses the host’s angiotensin-converting enzyme 2 (ACE2) receptor for cellular penetration, and the new structure of protein S 2019’s version increases the pathogeny of the virus [ 18 ]. None of the actual therapies are peptide-based, but based on SARS-CoV-1 studies, several molecules derived from protein S that target the ACE2 receptor have perspectives to be implemented [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection

et al. 2021

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Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.

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Peptides to combat viral infectious diseases

Cited by 44 publications

References 210 publications

Brilacidin Demonstrates Inhibition of SARS-CoV-2 in Cell Culture

Brilacidin Demonstrates Inhibition of SARS-CoV-2 in Cell Culture

Non-Toxic Dimeric Peptides Derived from the Bothropstoxin-I Are Potent SARS-CoV-2 and Papain-like Protease Inhibitors

Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection

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