Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection

Tertiş, Mihaela; Hosu, Oana; Feier, Bogdan; Cernat, Andreea; Florea, Anca; Cristea, Cécilia

doi:10.3390/molecules26113200

Cited by 31 publications

(15 citation statements)

References 79 publications

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“…Furthermore, the structure and stability of proteins are influenced by chemical interactions between them and their surrounding environment. 97 However, due to its lower likelihood of allosteric changes, linear peptide during immunoassay may be a more practical and reproducible option than secondary or tertiary ones. Epitope arrangement may be impacted by the conformational epitope during its production and preparation.…”

Section: Selection Modification and Synthesis Of Peptidementioning

confidence: 99%

“…Numerous noncovalent interactions among amino acids contribute to the stabilization of folded proteins. Furthermore, the structure and stability of proteins are influenced by chemical interactions between them and their surrounding environment . However, due to its lower likelihood of allosteric changes, linear peptide during immunoassay may be a more practical and reproducible option than secondary or tertiary ones.…”

Section: Selection Modification and Synthesis Of Peptidementioning

confidence: 99%

See 1 more Smart Citation

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Muslihati,

Septiani,

Gumilar

et al. 2024

ACS Biomater. Sci. Eng.

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In tropical and developing countries, mosquito-borne diseases by flaviviruses pose a serious threat to public health. Early detection is critical for preventing their spread, but conventional methods are time-consuming and require skilled technicians. Biosensors have been developed to address this issue, but cross-reactivity with other flaviviruses remains a challenge. Peptides are essentially biomaterials used in diagnostics that allow virological and serological techniques to identify flavivirus selectively. This biomaterial originated as a small protein consisting of two to 50 amino acid chains. They offer flexibility in chemical modification and can be easily synthesized and applied to living cells in the engineering process. Peptides could potentially be developed as robust, low-cost, sensitive, and selective receptors for detecting flaviviruses. However, modification and selection of the receptor agents are crucial to determine the effectiveness of binding between the targets and the receptors. This paper addresses two potential peptide nucleic acids (PNAs) and affinity peptides that can detect flavivirus from another target-based biosensor as well as the potential peptide behaviors of flaviviruses. The PNAs detect flaviviruses based on the nucleotide base sequence of the target's virological profile via Watson−Crick base pairing, while the affinity peptides sense the epitope or immunological profile of the targets. Recent developments in the functionalization of peptides for flavivirus biosensors are explored in this Review by division into electrochemical, optical, and other detection methods.

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Section: Selection Modification and Synthesis Of Peptidementioning

confidence: 99%

Section: Selection Modification and Synthesis Of Peptidementioning

confidence: 99%

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Muslihati,

Septiani,

Gumilar

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Biological or biomimetic receptor components, such as whole animals, [ 31 ] insect tentacles, [ 32 ] ORs, [ 33 ] odorant‐binding protein (OBP), [ 34 ] peptides, [ 35 ] olfactory cells and tissues, [ 36 ] molecularly imprinted polymers (MIPs), [ 37 ] as the element of the biosensors, allow a significant improvement of selectivity and specificity with simultaneous reduction of the problems associated with cross‐reactivity and complex sample matrix. The secondary transducers are non‐biological devices, which are used to convert and amplify biological signals.…”

Section: Development Of Bioelectronic Nosementioning

confidence: 99%

Artificial Olfactory Biohybrid System: An Evolving Sense of Smell

Qin

Wang

et al. 2022

Advanced Science

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The olfactory system can detect and recognize tens of thousands of volatile organic compounds (VOCs) at low concentrations in complex environments. Bioelectronic nose (B-EN), which mimics olfactory systems, is becoming an emerging sensing technology for identifying VOCs with sensitivity and specificity. B-ENs integrate electronic sensors with bioreceptors and pattern recognition technologies to enable medical diagnosis, public security, environmental monitoring, and food safety. However, there is currently no commercially available B-EN on the market. Apart from the high selectivity and sensitivity necessary for volatile organic compound analysis, commercial B-ENs must overcome issues impacting sensor operation and other problems associated with odor localization. The emergence of nanotechnology has provided a novel research concept for addressing these problems. In this work, the structure and operational mechanisms of biomimetic olfactory systems are discussed, with an emphasis on the development and immobilization of materials. Various biosensor applications and current developments are reviewed. Challenges and opportunities for fulfilling the potential of artificial olfactory biohybrid systems in fundamental and practical research are investigated in greater depth.

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“…Ad hoc developed peptides can bind proteins selectively, making it possible to identify proteins that are unique to a particular infection (7) (8). Peptides also possess stability against denaturation, satisfactory affinity and specificity, standard synthetic protocols, and ease of modification (9). Due to their many capabilities, they can be the adequate option as biological recognition components to achieve specific targeting for several different biological substances.…”

Section: Introductionmentioning

confidence: 99%

High performance photonics-based biosensing platform for COVID detection

Castaño

Zugaza

Zaballa

et al. 2023

Optical Sensors 2023

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The COVID-19 pandemic caused by the SARS-CoV-2 virus has brought global healthcare systems to their knees. The spike protein on the surface of the virus is a critical component for viral entry into human cells and therefore is a prime target for diagnosis and therapeutics. In recent years, the integration of photonic technology with biosensors has emerged as a promising approach due to its high sensitivity, specificity, and real-time detection capabilities. Optical fibres are one of the most versatile platforms for photonic technology-based biosensors, owing to their small size, low cost, and compatibility with various transduction methods. In this work, we present photonic technology based on optical fibres for the detection of the spike protein present in the SARS-CoV-2 virus. The proposed method involves the use of ad hoc synthesized peptides that specifically bind to the spike protein. The synthesized peptides are immobilized on the surface of the external face of an asymmetric Fabry-Perot cavity fabricated at the end face of a standard optical fibre, which acts as a biosensor. The presence of the spike protein in the sample causes a change in the refractive index, which is detected as variations in the visibility of the spectrum generated in Fabry-Perot cavity. The experimental results carried out have detected spike protein on buffered solutions with an LOD of 0.3 ng/ml. The proposed method offers several advantages over existing biosensors, including high sensitivity, real-time detection, and ease of integration into existing diagnostic platforms. We believe that the proposed photonic technology-based approach can significantly contribute to the development of biosensors for the early diagnosis of COVID-19 and other diseases.

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Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection

Cited by 31 publications

References 79 publications

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Artificial Olfactory Biohybrid System: An Evolving Sense of Smell

High performance photonics-based biosensing platform for COVID detection

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