Peptides from <i>Mucuna pruriens</i> L., with protection and antioxidant <i>in vitro</i> effect on HeLa cell line

Leo, Edwin E. Martínez; Martín-Ortega, Armando M; Acevedo-Fernández, Juan José; Moo-Puc, Rosa; Campos, Maira Rubí Segura

doi:10.1002/jsfa.9649

Cited by 18 publications

(11 citation statements)

References 36 publications

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“…Based on the methodology reported by Segura‐Campos, Salazar, Chel, & Betancur (2013), a protein‐rich fraction was obtained, which was subjected to an enzymatic hydrolysis process using a sequential pepsin‐pancreatin system, based on the method reported by Martínez‐Leo, Martín, Acevedo, Moo, and Segura‐Campos (2019). Protein‐rich fraction was adjusted to pH 2 using 6 N HCl and hydrolyzed by treatment with Pepsin (Sigma ® , P7000‐100G) for 45 min, followed by treatment with Pancreatin (Sigma ® , P3292‐100G) for another 45 min after adjusting to pH 7.5 using 6 N NaOH.…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective effect from Salvia hispanica peptide fractions on pro‐inflammatory modulation of HMC3 microglial cells

Leo

Campos

2020

J Food Biochem

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Neuroinflammation plays a critical role in the neurodegenerative disease's development, where microglia's act an important role in the mechanisms of response to neuronal damage. In the present research, the neuroprotective effect from Salvia hispanica peptide fractions on the proinflammatory modulation on HMC3 microglial cells was evaluated. From the enzymatic hydrolysis of a protein‐rich fraction from S. hispanica seeds, three peptide fractions (<1, 1–3 and 3–5 kDa) were obtained, from which its neuroprotective and anti‐inflammatory effect was determined on the production of proinflammatory mediators on HMC3 cells. The F1–3 kDa exhibited the greatest protective effect (79.04%), associated with the decrease in ROS cell production (51.3 ± 2.3%). Likewise, F1–3 kDa at 50 µg/ml, presented the highest reduction percentages of NO (33.1 ± 2.30%), TNFα (26.4 ± 1.1%) and IL6 (17.36 ± 1.6%). F1–3 kDa exhibited a neuroprotective effect in HMC3 cells associated with its antioxidant and anti‐inflammatory effect. Practical applications Currently, neurodegenerative diseases represent a global health problem, so the search for bioactive compounds with neuroprotective effect is useful in the prevention and treatment of this group of diseases. Peptide research with an effect on the proinflammatory and prooxidant mediator's reduction presents a potential application in the functional food's development aimed at the treatment of chronic diseases, that have oxidative stress and inflammation as their etiological factor. The present research adds to the scientific evidence of the potential benefits of bioactive peptides obtained from chia seeds. The results correlate with the main health benefits of whole chia seed in humans, such as antioxidant, anti‐inflammatory, hypoglycemic and hypotensive capacity. This relationship is associated with the protein and peptide composition of chia, which increases its added value as food.

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Section: Methodsmentioning

confidence: 99%

Neuroprotective effect from Salvia hispanica peptide fractions on pro‐inflammatory modulation of HMC3 microglial cells

Leo

Campos

2020

J Food Biochem

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“…The enzymatic hydrolysis was stopped by increasing the temperature to 80°C for 20 min. In order to eliminate the insoluble portion, the digested sample was centrifuged at 1,317 g for 20 min (Martínez‐Leo et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

“…Degree of hydrolysis (%) was measured via the following Equation (Martínez‐Leo et al., 2019):

DH (\% ) = \frac{Number of hydrolysed bonds (HBs)}{Total number of peptide bonds (PBs) per protein equivalent (Peq)} \times 100

…”

Section: Methodsmentioning

confidence: 99%

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Therapeutic peptides of Mucuna pruriens L.: Anti‐genotoxic molecules against human hepatocellular carcinoma and hepatitis C virus

Taghizadeh

Azizi

Asili

et al. 2021

Food Science & Nutrition

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To assist the development of new therapeutic strategies for several disorders, biologically active peptides/proteins obtained from plant sources can be considered. Current study expected to determine the biological activities of peptide fractions of Mucuna pruriens against hepatocellular carcinoma cell lines (HepG2/ADM, HepG2, SMMC‐7721, and QGY‐7703), as well as normal cell line to prove their selectivity. Moreover, anti‐genotoxicity and antiviral activity against the hepatitis C virus (HCV) were assessed. The methods of this study were to isolate the peptides of M. pruriens and hydrolysate fractionation via fractionated pepsin‐pancreatin hydrolysates by ultrafiltration/high‐performance ultrafiltration cell, identify anti‐hepatoma activity of peptide fractions human liver cancer and normal cells by (3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐biphenyl tetrazolium bromide) (MTT) assay, determine anti‐HCV, and assess anti‐genotoxic effect of peptide fractions against damage that induced via alkylating agent methyl methanesulphonate in human mononuclear cells. The results showed that the fraction 5–10 kDa has been reported to exhibit significant cytotoxic activity against HepG2 and QGY‐7703. It was proven that both of 5–10 and >10 kDa fractions are active against HCV. The cytotoxic concentration (CC50) of 5–10 kDa against the cell line was 703.04 ± 5.21 µg/ml. Anti‐genotoxic activities of the peptide fractions were evaluated as mean values for the analyzed comet images. In this regard, the highest activity of protecting DNA damages was observed by the peptide fraction of 5–10 kDa. This study revealed the potential ability of peptide fractions of M. pruriens for the treatment of liver cancer, HCV, and high activities of protecting DNA damages.

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“…The imbalance between oxidizing species and endogenous antioxidants causes oxidative stress. Moreover, oxidative stress has been associated with degenerative diseases like cardiovascular disorders and type II diabetes (Martinez-Leo et al, 2019). The intake of antioxidants could reduce the morbidity and mortality associated with coronary heart diseases, as demonstrated in several studies (Durak et al, 2013).…”

Section: Introductionmentioning

confidence: 98%

Antioxidant, angiotensin-converting enzyme, and α-amylase inhibitory activities of protein hydrolysates of Leucaena leucocephala seeds

Balderas-León

Baigts-Allende

Cardador‐Martínez

2021

CyTA - Journal of Food

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The Leucaena leucocephala seeds (LLS) cotyledon proteins, globulins, and glutelins were independently hydrolyzed by Alcalase, Trypsin, and α-Chymotrypsin up to 180 min. The degree of hydrolysis (DH), antioxidant, ACE-inhibitory, and α-Amylase inhibition were assessed. The higher DH values were 76.75% and 82.71% for globulin α-Chymotrypsin, and glutelin Alcalase hydrolysates, respectively. The glutelin hydrolyzed with Trypsin showed the higher DPPH • antioxidant activity while the higher ABTS antioxidant activity was for globulin Alcalase hydrolysate after 180 min digestion. The highest ACE-inhibitory activity was 95.44% for globulin-Alcalase hydrolysate at 180 min; while, for the α-Amylase inhibition assay, glutelin-Alcalase showed the highest values at 100 min of reaction. LLS cotyledon protein concentrates and hydrolysates with relatively low mimosine content were obtained after protein extraction. Such findings indicate the possibility of getting bioactive peptides from LLS cotyledon proteins using enzyme digestions and might be utilized for functional foods with physiological enhancer effects. RESUMENEl concentrado proteico y las fracciones de globulinas y glutelinas del cotiledón de semillas de Leucaena leucocephala (LLS) fueron hidrolizados con Alcalasa, Tripsina y α-quimotripsina hasta 180 min. El grado de hidrólisis (DH), actividades antioxidante e inhibitoria de las enzimas convertidora de angiotensina (ACE-I) y α-amilasa fueron medidas. Los grados de hidrólisis más altos fueron 76.75% y 82.71% para los hidrolizados de globulina-α quimotripsina y glutelina-Alcalasa, respectivamente. El hidrolizado de glutelina con Tripsina mostró la mayor actividad antioxidante con DPPH, mientras que el hidrolizado de globulina con alcalasa fue el más alto a los 180 min en el método ABTS. La mayor actividad de ACE-I (95.44%) se observó en el hidrolizado de globulinas-Alcalasa. Por el contrario, el hidrolizado de glutelinas-Alcalasa a los 100 min mostró la mayor actividad. Se obtuvieron concentrados proteicos e hidrolizados con un contenido de mimosina relativamente bajo. Estos resultados indican la posibilidad de obtener péptidos bioactivos a partir del cotiledón de LLS usando digestión enzimática los cuales podrían utilizarse en alimentos funcionales con efectos fisiológicos.

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Peptides from Mucuna pruriens L., with protection and antioxidant in vitro effect on HeLa cell line

Cited by 18 publications

References 36 publications

Neuroprotective effect from Salvia hispanica peptide fractions on pro‐inflammatory modulation of HMC3 microglial cells

Neuroprotective effect from Salvia hispanica peptide fractions on pro‐inflammatory modulation of HMC3 microglial cells

Therapeutic peptides of Mucuna pruriens L.: Anti‐genotoxic molecules against human hepatocellular carcinoma and hepatitis C virus

Antioxidant, angiotensin-converting enzyme, and α-amylase inhibitory activities of protein hydrolysates of Leucaena leucocephala seeds

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