Peptide Regulation of Skin Fibroblast Functions during Their Aging In Vitro

Linkova, N. S.; Дробинцева, А О; Орлова, О. А.; Кузнецова, Е. М.; Polyakova, V. O.; Kvetnoy, I. M.; Khavinson, V. Kh.

doi:10.1007/s10517-016-3370-x

Cited by 14 publications

(9 citation statements)

References 9 publications

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“…MTT test showed that AED, KED, KE, and AEDG peptides and their combination stimulate hPDLSCs proliferation. It has been reported that KE peptide and AED peptide, which stimulate proliferation of immune cells and fibroblasts, 27,31 can potentiate proliferation activity and have a proliferation effect in hPDLSCs. KED peptide in previous investigation decreased stem cell proliferation, but stimulates thymocytes and skin fibroblast proliferation.…”

Section: Discussionmentioning

confidence: 99%

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Effect of short peptides on neuronal differentiation of stem cells

Caputi

Trubiani

Sinjari

et al. 2019

Int J Immunopathol Pharmacol

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It has been demonstrated that short peptides play an important role in the transmission of biological information, modulation of transcription, and restoring genetically conditioned alterations occurring with age. Peptidergic regulation of homeostasis occupies an important place in physiological processes, which lead to the aging of cells, tissues, and organs, consisting in the involution of major regulatory systems-the nervous, the endocrine, and the immune. The effect of AED (Ala-Glu-Asp), KED (Lys-Glu-Asp), KE (Lys-Glu), AEDG (Ala-Glu-Asp-Gly) peptides and their compound on neuronal differentiation of human periodontal ligament stem cells (hPDLSCs) was studied by immunofluorescence and western blot analysis. Growth-Associated Protein 43 (GAP43), which implements neurotransmission mechanisms and neuroplasticity, demonstrated an increased expression in hPDLSCs cultured with a compound of all studied peptides and with KED alone. The peptide compound and KED, increase the expression of Nestin (neurofilament protein), expressed in early neuronal precursors in hPDLSCs cultures. Thus, the compound of peptides AEDG, KE, AED, and KED could promote the neuronal differentiation of hPDLSCs and be a promising tool for the study of peptides as a modulator of neurogenesis in neurodegenerative diseases studied in animal models.

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Section: Discussionmentioning

confidence: 99%

“…Decreased MMP9 expression and increased Ki67 and CD98hc expression in primary rat's skin fibroblasts. 27 7. Vasoprotective effect in human and rat's vessel.…”

Section: Mtt Assaymentioning

confidence: 99%

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Effect of short peptides on neuronal differentiation of stem cells

Caputi

Trubiani

Sinjari

et al. 2019

Int J Immunopathol Pharmacol

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“…Полученные данные дополняют результаты ранее проведенных исследований в диссоциированной культуре фибробластов кожи крыс при ее репликативном старении. В этой работе было установлено, что пептид KED уменьшает продукцию матриксной металлопротеиназы-9 (ММР9) и стимулирует синтез молекул, активирующих пролиферацию и замедляющих старение фибробластов кожи (Ki67, CD98hc) [3]. Кроме того, применение пептида KED в сочетании с другими пептидами путем электрофореза повышало толщину дермы и эпидермиса у женщин среднего возраста [14].…”

Section: материалы и методы исследованияunclassified

Effect of Vasoprotective Peptides on Apoptosis of Organotypic Rat Skin Culture

Линькова¹,

Chalisova²,

Ryzhak³

et al. 2021

СПНО (MPSE)

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Замедление инволютивных процессов в коже является актуальной задачей геронтокосметологии. В статье рассматриваются пептиды и аминокислоты, регулирующие функциональную активность клеток кожи. Методом иммуноцитохимии изучено влияние аминокислот лизина (Lys), глутаминовой кислоты (Glu), аспарагиновой кислоты (Asp), пептида KED (Lys-Glu-Asp) и полипептидного комплекса сосудов на экспрессию проапоптотических белков р53, р21, р16, р65 в органотипической культуре кожи крыс. Полипептидный комплекс сосудов и пептид KED оказывали более сильный антиапоптотический эффект на клетки кожи по сравнению с L-аминокислотами. Полипептидный комплекс сосудов и пептид KED соответственно на 39-69% и на 40-65% снижали экспрессию белков р53, р21, р16, р65 в культуре клеток кожи, т.е. их влияние на клетки кожи было сопоставимо. Можно полагать, действие полипептидного комплекса сосудов на клетки кожи достигается за счет пептида KED в его составе. Уменьшение экспрессии проапоптотических протеинов может указывать на способность пептида KED и полипептидного комплекса сосудов поддерживать функциональную активность фибробластов кожи при старении. Полученные данные указывают на перспективность дальнейшего изучения полипептидного комплекса сосудов и пептида KED в качестве геропротекторов, поддерживающих функциональную активность клеток кожи. Ключевые слова: апоптоз, кожа, культура клеток, пептид KED, полипептидный комплекс сосудов.

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“…These short “cryptic” peptides can be formed endogenously by proteolytic cleavage of nuclear proteins or by synthesis after which they penetrate the cytoplasmic and nuclear membrane [60, 61]. These peptides are involved in the epigenetic regulation of aging and can have health-promoting effects by for example suppressing age-related increased expression of matrix metalloproteases and caspase-dependent apoptosis [55, 93].…”

Section: The Influence Of Peptides On Epigenetic Mechanismsmentioning

confidence: 99%

Peptides as epigenetic modulators: therapeutic implications

2019

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Peptides originating from different sources (endogenous, food derived, environmental, and synthetic) are able to influence different aspects of epigenetic regulation. Endogenous short peptides, resulting from proteolytic cleavage of proteins or upon translation of non-annotated out of frame transcripts, can block DNA methylation and hereby regulate gene expression. Peptides entering the body by digestion of food-related proteins can modulate DNA methylation and/or histone acetylation while environmental peptides, synthesized by bacteria, fungi, and marine sponges, mainly inhibit histone deacetylation. In addition, synthetic peptides that reverse or inhibit different epigenetic modifications of both histones and the DNA can be developed as well. Next to these DNA and histone modifications, peptides can also influence the expression of non-coding RNAs such as lncRNAs and the maturation of miRNAs. Seen the advantages over small molecules, the development of peptide therapeutics is an interesting approach to treat diseases with a strong epigenetic basis like cancer and Alzheimer’s disease. To date, only a limited number of drugs with a proven epigenetic mechanism of action have been approved by the FDA of which two (romidepsin and nesiritide) are peptides. A large knowledge gap concerning epigenetic effects of peptides is present, and this class of molecules deserves more attention in the development as epigenetic modulators. In addition, none of the currently approved peptide drugs are under investigation for their potential effects on epigenetics, hampering drug repositioning of these peptides to other indications with an epigenetic etiology.

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Peptide Regulation of Skin Fibroblast Functions during Their Aging In Vitro

Cited by 14 publications

References 9 publications

Effect of short peptides on neuronal differentiation of stem cells

Effect of short peptides on neuronal differentiation of stem cells

Effect of Vasoprotective Peptides on Apoptosis of Organotypic Rat Skin Culture

Peptides as epigenetic modulators: therapeutic implications

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