Peptide nucleic acids on microarrays and other biosensors

Brandt, Ole; Hoheisel, Jörg D.

doi:10.1016/j.tibtech.2004.10.003

Cited by 102 publications

(67 citation statements)

References 44 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of the superior properties of PNA, the PNA array gives rise to higher specificity, higher sensitivity, and higher stability than the DNA array in the detection of target sequences. Therefore, the PNA array is important for diagnostic methods (3,6,7,24).…”

Section: Discussionmentioning

confidence: 99%

“…These properties of PNA are widely used as a molecular tool in molecular biology and biotechnology. An increasing number of applications for PNA technology have been described, detecting specific gene sequences in advanced diagnostic methods such as PCR clamping, real-time PCR, FISH (fluorescence in situ hybridization), and microarrays (3,4,10,24). The PNA array (for research use only, not for use in diagnostic procedures) (Panagene Inc., Daejeon, South Korea) is an in vitro microarray used to detect the presence of different genetic variants of HBV in human blood samples (PANArray DR HBV).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Peptide Nucleic Acid Array for Detection of Point Mutations in Hepatitis B Virus Associated with Antiviral Resistance

Jang¹,

Kim²,

Choi³

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

The detection of antiviral-resistant hepatitis B virus (HBV) mutations is important for monitoring the response to treatment and for effective treatment decisions. We have developed an array using peptide nucleic acid (PNA) probes to detect point mutations in HBV associated with antiviral resistance. PNA probes were designed to detect mutations associated with resistance to lamivudine, adefovir, and entecavir. The PNA array assay was sensitive enough to detect 10 2 copies/ml. The PNA array assay was able to detect mutants present in more than 5% of the virus population when the total HBV DNA concentration was greater than 10 4 copies/ml. We analyzed a total of 68 clinical samples by this assay and validated its usefulness by comparing results to those of the sequencing method. The PNA array correctly identified viral mutants and has high concordance (98.3%) with direct sequencing in detecting antiviral-resistant mutations. Our results showed that the PNA array is a rapid, sensitive, and easily applicable assay for the detection of antiviral-resistant mutation in HBV. Thus, the PNA array is a useful and powerful diagnostic tool for the detection of point mutations or polymorphisms.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Peptide Nucleic Acid Array for Detection of Point Mutations in Hepatitis B Virus Associated with Antiviral Resistance

Jang¹,

Kim²,

Choi³

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…Winzor and Chan and Chen highlight the biosensor's contributions in affinity chromatography [93] and characterization of protein-fouled membranes [11]. Brandt and Hoheisel, Campàs and Katakis, Disney and Seeberger, Espina et al, Michaud, Pavlivkova et al, and Walsh and Chang each discuss the applicability of SPR as a detection method in the development of higherthroughput protein, carbohydrate, DNA and small molecule array platforms [6,8,24,27,59,68,90], Lion et al summarizes the importance of microfluidics in Biacore biosensors [49], and Buijs and Natsume, Damle et al, Mattei et al and Stuhler and Meyer describe coupling the biosensor with mass spectrometry to streamline isolation and identification of bound analytes from crude materials [7,18,57,84].…”

Section: Reviews Theory and Methodsmentioning

confidence: 99%

Survey of the year 2004 commercial optical biosensor literature

Rich

Myszka

2005

J of Molecular Recognition

114

View full text Add to dashboard Cite

The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent a $ 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe wellperformed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor-and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.

show abstract

“…In Arlinghaus et al work, ToF-SIMS was used to monitor the amount of immobilised PNA on platinum-coated silicon chips, and thus check the sample reproducibility. These chips were then hybridised and analysed for phosphorous signal; a peak was observed in the case of complementary (Brandt & Hoheisel, 2004). …”

Section: Time-of-flight Ion Mass Spectrometry (Tof Sims)mentioning

confidence: 99%

“…In the latter case, DNA binding to PNA induces a change in the refractive index, proportional to the mass uptake on the chip surface and enabling a real-time monitoring of the hybridisation process (Jensen et al, 1997). A small review of some PNA-based biosensors has been recently published, presenting some examples of biosensors using labels or not, and showing the interest of using these DNA mimics (Brandt & Hoheisel, 2004;Corradini et al, 2004); they report in particular that PNA monomers may be modified by adding a chiral center to enhance their mismatch recognition sensitivity by SPR (Corradini et al, 2004); also, instead of using the direct grafted PNA format, PCR products may be attached to the SPR sensor surface, followed by PNA oligomer hybridisation leading to highly efficient PNA hybridisation; this has been attributed to the better accessibility of PNA probes to the immobilised PCR, compared to that of DNA oligonucleotides (Feriotto et al, 2001). However, the SPR detection sensitivity often needs to be enhanced by incorporating labels or using a second hybridisation (sandwich format).…”

Section: Time-of-flight Ion Mass Spectrometry (Tof Sims)mentioning

confidence: 99%