A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques

Mateo‐Martí, Eva; Pradier, Claire‐Marie; Jussieu, Pl

doi:10.5772/7160

Cited by 4 publications

(4 citation statements)

References 67 publications

(64 reference statements)

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“…Moreover, the stability constants of complexes of native nucleic acid “hosts” with their cognate ligand “guests” are relatively low, being on the order of 10 3 M –1 . Successfully, the presently determined K s TTTATA–TATAAA value for the hexamer probe is comparable to those for much longer-chain DNA–PNA hybrids, 10 6 M –1 (for PNA-GCAT TT GCAT) ≤ K s DNA–PNA ≤ 10 7 M –1 (for PNA-GCAT GA GCAT). , Our procedure circumvents the disadvantages connected with a very low stability of short ON hybrids.…”

Section: Discussionmentioning

confidence: 78%

“…63 Successfully, the presently determined K s TTTATA-TATAAA value for the hexamer probe is comparable to those for much longer-chain DNA-PNA hybrids, 10 6 M -1 (for PNA-GCATTTGCAT) ≤ K s DNA-PNA ≤ 10 7 M -1 (for PNA-GCATGAGCAT). 64,65 Our procedure circumvents disadvantages connected with a very low stability of short ON hybrids. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 With the presently developed procedure, we fabricated an oligomer analog of DNA in the molecular cavities of MIPs.…”

Section: Discussionmentioning

confidence: 99%

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Programmed Transfer of Sequence Information into a Molecularly Imprinted Polymer for Hexakis(2,2′-bithien-5-yl) DNA Analogue Formation toward Single-Nucleotide-Polymorphism Detection

Bartold

Pietrzyk‐Le

Huynh

et al. 2017

ACS Appl. Mater. Interfaces

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A new strategy of simple, inexpensive, rapid, and label-free single-nucleotide-polymorphism (SNP) detection using robust chemosensors with piezomicrogravimetric, surface plasmon resonance, or capacitive impedimetry (CI) signal transduction is reported. Using these chemosensors, selective detection of a genetically relevant oligonucleotide under FIA conditions within 2 min is accomplished. An invulnerable-to-nonspecific interaction molecularly imprinted polymer (MIP) with electrochemically synthesized probes of hexameric 2,2'-bithien-5-yl DNA analogues discriminating single purine-nucleobase mismatch at room temperature was used. With density functional theory modeling, the synthetic procedures developed, and isothermal titration calorimetry quantification, adenine (A)- or thymine (T)-substituted 2,2'-bithien-5-yl functional monomers capable of Watson-Crick nucleobase pairing with the TATAAA oligodeoxyribonucleotide template or its peptide nucleic acid (PNA) analogue were designed. Characterized by spectroscopic techniques, molecular cavities exposed the ordered nucleobases on the 2,2'-bithien-5-yl polymeric backbone of the TTTATA hexamer probe designed to hybridize the complementary TATAAA template. In that way, an artificial TATAAA-promoter sequence was formed in the MIP. The purine nucleobases of this sequence are known to be recognized by RNA polymerase to initiate the transcription in eukaryotes. The hexamer strongly hybridized TATAAA with the complex stability constant K = k/k ≈ 10 M, as high as that characteristic for longer-chain DNA-PNA hybrids. The CI chemosensor revealed a 5 nM limit of detection, quite appreciable as for the hexadeoxyribonucleotide. Molecular imprinting increased the chemosensor sensitivity to the TATAAA analyte by over 4 times compared to that of the nonimprinted polymer. The herein-devised detection platform enabled the generation of a library of hexamer probes for typing the majority of SNP probes as well as studying a molecular mechanism of the complex transcription machinery, physics of single polymer molecules, and stable genetic nanomaterials.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Programmed Transfer of Sequence Information into a Molecularly Imprinted Polymer for Hexakis(2,2′-bithien-5-yl) DNA Analogue Formation toward Single-Nucleotide-Polymorphism Detection

Bartold

Pietrzyk‐Le

Huynh

et al. 2017

ACS Appl. Mater. Interfaces

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“…Figure a depicts the structure of PNA/DNA hybridization with the N -(2-aminoethyl)-glycine backbone of PNA hybridized in an antiparallel direction by the deoxyribose-phosphate backbone of DNA. The hydrogen bonds exist between complementary nucleobases and show different backbone linkages between PNA and DNA …”

Section: Selection Modification and Synthesis Of Peptidementioning

confidence: 99%

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Muslihati,

Septiani,

Gumilar

et al. 2024

ACS Biomater. Sci. Eng.

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In tropical and developing countries, mosquito-borne diseases by flaviviruses pose a serious threat to public health. Early detection is critical for preventing their spread, but conventional methods are time-consuming and require skilled technicians. Biosensors have been developed to address this issue, but cross-reactivity with other flaviviruses remains a challenge. Peptides are essentially biomaterials used in diagnostics that allow virological and serological techniques to identify flavivirus selectively. This biomaterial originated as a small protein consisting of two to 50 amino acid chains. They offer flexibility in chemical modification and can be easily synthesized and applied to living cells in the engineering process. Peptides could potentially be developed as robust, low-cost, sensitive, and selective receptors for detecting flaviviruses. However, modification and selection of the receptor agents are crucial to determine the effectiveness of binding between the targets and the receptors. This paper addresses two potential peptide nucleic acids (PNAs) and affinity peptides that can detect flavivirus from another target-based biosensor as well as the potential peptide behaviors of flaviviruses. The PNAs detect flaviviruses based on the nucleotide base sequence of the target's virological profile via Watson−Crick base pairing, while the affinity peptides sense the epitope or immunological profile of the targets. Recent developments in the functionalization of peptides for flavivirus biosensors are explored in this Review by division into electrochemical, optical, and other detection methods.

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“…Due to the lack of electrostatic repulsion, existing between two DNA oligonucleotides, the binding between PNA and DNA is even stronger and more specific than the formation of DNA duplexes. Thus, PNA is considered to offer extraordinary specific detection of DNA to facilitate the identification of single-nucleotide mismatches [53].…”

Section: Dna and Pnamentioning

confidence: 99%

Porous Silicon Biosensors Employing Emerging Capture Probes

Urmann

Tenenbaum

Walter

et al. 2015

Electrochemically Engineered Nanoporous Materials

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The application of porous silicon (PSi) for biosensing was first described by Thust et al. in 1996, demonstrating a potentiometric biosensor for the detection of penicillin. However, only in the past decade PSi has established as a promising nanomaterial for label-free biosensing applications. This chapter focuses on the integration of new emerging capture probes with PSi-based biosensing schemes. An overview of natural and synthetic receptors and their advantageous characteristics for the potential application in PSi biosensors technology is presented. We also review and discuss several examples, which successfully combine these new bioreceptors with PSi optical and electrochemical transducers, for label-free biosensing.

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A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques

Cited by 4 publications

References 67 publications

Programmed Transfer of Sequence Information into a Molecularly Imprinted Polymer for Hexakis(2,2′-bithien-5-yl) DNA Analogue Formation toward Single-Nucleotide-Polymorphism Detection

Programmed Transfer of Sequence Information into a Molecularly Imprinted Polymer for Hexakis(2,2′-bithien-5-yl) DNA Analogue Formation toward Single-Nucleotide-Polymorphism Detection

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Porous Silicon Biosensors Employing Emerging Capture Probes

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