Peptide nucleic acids inhibit growth of Brucella suis in pure culture and in infected murine macrophages

Rajasekaran, P.; Alexander, Jeffry; Seleem, Mohamed N.; Jain, Neeta; Sriranganathan, Nammalwar; Wattam, Alice R.; Setúbal, João Carlos; Boyle, Stephen M.

doi:10.1016/j.ijantimicag.2012.11.017

Cited by 38 publications

(39 citation statements)

References 22 publications

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“…Rajasekaran et al have studied the inhibition of Brucella suis growth using PNA linked covalently to cell-penetrating peptides (CPPs) [2]. After 24 hour of treatment, Brucella genes are involved in DNA, RNA, cell envelope, fatty acid and protein synthesis that are inhibited the growth of B. suis in culture and macrophages.…”

Section: Applications Of Pnamentioning

confidence: 99%

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A Review of Peptide Nucleic Acid

Siddiquee¹,

Rovina²,

Azriah³

2015

Adv Tech Biol Med

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Peptide Nucleic Acid (PNA) is a nucleobase oligomer in which the whole backbone is mainly replaced by N-(2aminoethyl) glycine units. PNA is considered as DNA with a neutral peptide backbone due to negative charged sugar-phosphate backbone. It is chemically stable and resistant to hydrolytic cleavage. PNA can be categorized specific sequences of DNA and RNA according to Watson-Crick hydrogen bonding structure. Hybridization process showed high thermal stability and unique ionic strength effects. It is formed a stable PNA/DNA/PNA triplex with a looped-out DNA strand. PNA hybridization technology is promptly developed within in situ hybridization. In our review paper was elaborated the PNA superior hybridization characteristics, importance's of PNA and major applications of PNA in the diagnostic and pharmaceutical fields. And also PNA could be replaced DNA in uses as a probe for many investigation purposes. PNAs antisense activities have found in nerve cells and even in rats upon injection into the brain, and in Escherichia coli.

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Section: Applications Of Pnamentioning

confidence: 99%

“…Peptide Nucleic Acid (PNA) are single stranded which potentially mimic DNA capable of hybridizing to complementary DNA, RNA or PNA [2,3]. PNAs have high chemical stability and specificity [4,5].…”

Section: Introductionmentioning

confidence: 99%

A Review of Peptide Nucleic Acid

Siddiquee¹,

Rovina²,

Azriah³

2015

Adv Tech Biol Med

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“…Results are expressed as mean ± SEM of several independent experiments. engineering has been established for Brucella (Rajasekaran et al 2013). They have used peptide nucleic acids to inhibit the growth of Brucella suis in pure culture and in infected murine macrophages.…”

Section: Discussionmentioning

confidence: 99%

A new method for simultaneous gene deletion and down-regulation in Brucella melitensis Rev.1

Saeedinia

Zeinoddini

Soleimani

et al. 2015

Microbiological Research

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In this study, our aim was to integrate an antisense expression cassette in bacterial chromosome for providing a long-term expression down-regulation in a bid to develop a new approach for simultaneous deletion and down-regulation of target genes in bacterial system. Therefore, we were used this approach for simultaneous deletion of the perosamine synthetase (per) gene and down-regulation of the virB1 expression in Brucella melitensis Rev.1. The per gene, which is one of the LPS O-chain coding genes, was replaced by homologous recombination with an antisense virB1 expression cassette together with kanamycin resistance cassette (kan(R)). Deletion of the per gene was characterized by PCR analysis and DNA sequencing. The expression of antisense virB1 cassette was confirmed by RT-PCR. Down-regulation of the virB1 mRNA expression was quantified by real-time RT-PCR using virB1 specific primers relative to the groEL reference gene. The survival rate of mutant strain was evaluated by CFU count in the BALB/c mice. The virB1 mRNA expression was down-regulated on average 10-fold in mutant strain as compared to parental strain. The loss of per gene function and decrease of the virB1 mRNA expression resulted in reduced entry and survival of the mutant Rev.1 strain in BALB/c mice splenocytes. We propose that this method can be used for simultaneous regulation of multiple genes expression.

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“…They readily cross the bacterial cell membranes when coupled with a cell-penetrating peptide. Rajasekaran et al [97] reported growth inhibition of B. suis by PNAs, both in cell-free medium and in murine macrophages. In tryptic soy broth, the inhibitory PNAs were those targeting the genes kdtA (coding for a transferase affecting lipid A), tsf (elongation factor Ts), polA (DNA polymerase I), and rpoB (subunit B of RNA polymerase).…”

Section: Peptide Nucleic Acidsmentioning

confidence: 99%

Antibiotic Susceptibility Testing of Brucella Species - Old and New Drugs

Maurin¹

2015

Updates on Brucellosis

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Brucella species cause brucellosis in humans and animals, a zoonosis that can manifest not only as acute or chronic diseases but also as silent infections persisting throughout life with recurrences potentially occurring after several decades. In vitro and in vivo methods have been developed to evaluate the bacteriostatic and bactericidal activity of antibiotics against Brucella sp. Especially eukaryotic cells and animal models have been used to eval uate the ability of antibiotics, alone or in combination, to eradicate these bacteria from their intracellular reservoir. Although treatment recommendations have been established for common clinical forms of brucellosis, optimized therapeutic alternatives are still need ed for severe forms of the disease, and for infections occurring in young children and pregnant women. Moreover, acquired resistance to first-line treatments of brucellosis is a current concern. This chapter will summarize current knowledge on in vitro and in vivo interactions between Brucella species and antibiotics and new therapeutic strategies that have been evaluated. Keywords: Brucella, brucellosis, antibiotic susceptibility testing, antibiotic resistance, treatment . IntroductionMost Brucella species are highly infectious in humans and thus are considered class biological agents [ -] and potential biological threat agents by the CDC class B [ , ]. Because of a high risk of human infections, especially through inhalation of infectious aerosols, the Brucella cultures should be handled in a biosafety level laboratory. Also, in many countries, detention of these pathogens is now subject to strict regulations. The clinical symptoms of brucellosis are often unspecific. Therefore, the diagnosis may be delayed, especially in geographic areas where the disease is rare and thus often not evoked by physicians in febrile patients. A definite © 2015 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. diagnosis of brucellosis relies on isolation of Brucella sp. from infected patients, mainly from blood samples during the first few weeks following the onset of symptoms. Serological methods lack specificity, and only represent a stopgap for brucellosis diagnosis. PCR-based techniques are useful to detect Brucella DNA in clinical samples, especially in patients with suppurated secondary locations. A specific antibiotic therapy should be started as soon as possible to avoid severe complications including neurological and cardiac involvement , and evolution to a chronic debilitating disease. However, current treatment alternatives are still scarce in adult patients, and even more limited in young children and pregnant women. Although rarely fatal, brucellosis remains a major public health problem worldwide, and a significant economic burden in livestock because of its a...

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Peptide nucleic acids inhibit growth of Brucella suis in pure culture and in infected murine macrophages

Cited by 38 publications

References 22 publications

A Review of Peptide Nucleic Acid

A Review of Peptide Nucleic Acid

A new method for simultaneous gene deletion and down-regulation in Brucella melitensis Rev.1

Antibiotic Susceptibility Testing of Brucella Species - Old and New Drugs

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