Peptide molecular weight distribution of soluble protein fraction affects growth performance and quality in European sea bass (Dicentrarchus labrax) larvae

Abstract

Dicentrarchus labrax were fed from 10 to 40 days posthatching with six microdiets differing in the inclusion level (60 g kg‐1 and 120 g kg‐1) and type of protein hydrolysate (PH; yeast, YPH; pig blood, PBPH; pig red blood cells, PRBCPH). A microdiet containing 120 g kg‐1 fish PH (FPH) was used as a control. PH differed in their amino acid (AA) profile and molecular weight distribution and therefore the tested microdiets too. The estimated content in FAA and di‐ and tripeptides in the FPH microdiet was 2 g kg‐1 and 44 g kg‐1, respectively. FAA estimated levels in YPH and PBPH microdiets were 26 g kg‐1 and 53 g kg‐1, whereas levels of di‐ and tripeptides were 30 g kg‐1 and 60 g kg‐1, respectively. The estimated levels of FAA in PRBCPH microdiets were 8 g kg‐1 and 17 g kg‐1, whereas estimated levels of di‐ and tripeptides were 11 g kg‐1 and 22 g kg‐1, respectively. Results revealed that FPH may be replaced by alternative PH from yeast and pig blood products, as fish fed those diets performed, in terms of growth, survival, digestive function and incidence of skeletal deformities, as well as those fed the FPH microdiet. Using YPH, PBPH and PRBCPH, the inclusion level of PH in microdiets might be reduced to a half with respect to current practices using FPH.

The effect of ultrafiltered fish protein hydrolysate level on growth performance, protein digestibility and mRNA expression of PepT1 in juvenile turbot (Scophthalmus maximusL.)

“…This result has been reported for several times in different species such as Japanese founder (Paralichthys olivaceus) (Zheng et al 2012) and Atlantic salmon (Salmo salar L.; Espe et al 1999;Refstie et al 2004;Hevrøy et al 2005). Peptide molecular distribution of protein hydrolysates may affect fish growth performance (Skalli et al 2014), and especially, small molecular weight may play an important role in growth for fish fed high plant protein diets (Aksnes et al 2006b,c;Kousoulaki et al 2009;Zheng et al 2012).…”

“…Peptide molecular distribution of protein hydrolysates may affect fish growth performance (Skalli et al . ), and especially, small molecular weight may play an important role in growth for fish fed high plant protein diets (Aksnes et al . ,c; Kousoulaki et al .…”

Frações solúveis e insolúveis do hidrolisado proteico de resíduo de sardinha na alimentação do jundiá: consumo de ração e excreção de amônia

“…Outro aspecto a ser considerado diz respeito ao nível de inclusão dos hidrolisados neste estudo (20%), maior do que os tradicionalmente testados, de 5 a 15% (Aksnes et al, 2006;Zheng et al, 2013;Skalli et al, 2014). Isso levou a um desbalanceamento do perfil de aminoácidos da dieta contendo somente a fração solúvel e gerou deficiência em alguns aminoácidos essenciais (lisina, metionina e treonina) na dieta contendo apenas a fração solúvel (Liaset e Espe, 2008).…”

“…Entretanto, o efeito dessas frações, combinadas ou isoladas, ainda não foi devidamente estudado na alimentação de peixes. De maneira geral, a maioria dos trabalhos avalia somente a fração solúvel filtrada dos hidrolisados (Aksnes et al, 2006;Skalli et al, 2014;CostaBonfim et al, 2016).…”

Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets

“…Under conditions of nutritional stress, the expression of some AA transporters (y + LAT1) may be increased to maintain normal growth of animals under adverse conditions (He et al, 2013). Skalli et al (2014) suggest that dietary protein levels regulate the expression of AA transporter genes by a complex regulatory system, which may also affect the energy balance and endocrine system. AAs and hormones can play an important role in the regulation of gene expression.…”