Peptide hydrogels

Dasgupta, Antara; Mondal, Julfikar Hassan; Das, Debapratim

doi:10.1039/c3ra40234g

Cited by 289 publications

(248 citation statements)

References 288 publications

(534 reference statements)

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“…An alternating sequence of hydrophobic and hydrophilic amino acid sequences and positively and negatively charged peptides results in the formation of a stable hydrogel under controlled pH and ionic conditions [64][65][66][67]. These types of hydrogel are particularly useful in examining cellular behaviour owing to their biocompatibility, biodegradability and biofunctionality [68]. cornea agarose-fibrin [29] alginate-gelatin nanofibre [163] collagen [146,149,150] collagen-PLGLA nanofibre [160] alginate [34] cellulose [35] heparin [36] alginate-hyaluronic acid [33] collagen [190] collagen-chitosan [32] dextran [30,31] hyaluronic acid [44] alginate [27] collagen [23,24] dextran [25] gelatin [26] collagen [17,97] fibrin [37] agarose [51,175,179,196] alginate [126] chitosan [20,50] fibrin [42,51] gellan gum [22,51] hyaluronic acid [110] PEG [60] A key factor when examining cell-material mechano-interactions is the mechanisms of cell adhesion to the hydrogel.…”

Section: Cell-mediated Remodelling Of Hydrogelsmentioning

confidence: 99%

Introduction to cell–hydrogel mechanosensing

2014

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The development of hydrogel-based biomaterials represents a promising approach to generating new strategies for tissue engineering and regenerative medicine. In order to develop more sophisticated cell-seeded hydrogel constructs, it is important to understand how cells mechanically interact with hydrogels. In this paper, we review the mechanisms by which cells remodel hydrogels, the influence that the hydrogel mechanical and structural properties have on cell behaviour and the role of mechanical stimulation in cell-seeded hydrogels. Cell-mediated remodelling of hydrogels is directed by several cellular processes, including adhesion, migration, contraction, degradation and extracellular matrix deposition. Variations in hydrogel stiffness, density, composition, orientation and viscoelastic characteristics all affect cell activity and phenotype. The application of mechanical force on cells encapsulated in hydrogels can also instigate changes in cell behaviour. By improving our understanding of cell-material mechano-interactions in hydrogels, this should enable a new generation of regenerative medical therapies to be developed.

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Section: Cell-mediated Remodelling Of Hydrogelsmentioning

confidence: 99%

Introduction to cell–hydrogel mechanosensing

2014

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“…11 Among these gelators, the biocompatible and biodegradable species are particularly popular for constructing hydrogels, such as nucleobases, 12 bile salts, 13 carbohydrates 14 and peptides, 15 as they can be safely used in biological applications. 16 Bile acids and their salts belong to not only important biological surfactants, but also small-molecule biological gelators that can act as solubilizers of cholesterol, bilirubin, and various fat-soluble vitamins in the intestine of vertebrates, including humans.…”

Section: Introductionmentioning

confidence: 99%

Effects of polymers on the properties of hydrogels constructed using sodium deoxycholate and amino acid

et al. 2018

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The gelation behavior and properties of sodium deoxycholate (NaDC) and L-aspartic acid (Asp) in aqueous solution were investigated in detail at 25 C. The linear polymer poly(2-(2-methoxyethoxy)ethyl methacrylate-co-oligo-(ethylene glycol) methacrylate) (P(MEO 2 MA 90 -co-OEGMA 10 )) and star-shaped polymer poly(2-(dimethylamino)ethyl methacrylate-b-2-(2-methoxyethoxy)ethyl methacrylate) (CDPDPM) were introduced in NaDC/Asp hydrogels for exploring the effects of polymers on the properties of NaDC/Asp hydrogels and the mechanism underlying gelation processes by polymers was proposed. The hydrogels were characterized by phase behavior observation, polarized optical microscopy (POM), cryogenic scanning electron microscopy (cryo-SEM), X-ray powder diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy and rheological measurements. Moreover, the adsorption performances of hydrogels with and without polymers to methylene blue (MB) were studied using a UV-vis spectrometer. The results indicated that the transition from sol to gel state was observed with an increase in the Asp concentration in the system. Both linear and star-shaped polymers can participate in the formation of a gel network structure, so that the density of network structure and the mechanical strength of hydrogels increased. Furthermore, it was found that the viscoelasticity of the CDPDPM-containing hydrogel was much higher than that of the P(MEO 2 MA 90 -co-OEGMA 10 )-containing hydrogel under the same condition, indicating that CDPDPM performed better in strengthening the network structure of the hydrogels than P(MEO 2 MA 90 -co-OEGMA 10 ) due to the special structure that provided more binding sites for hydrogen bonding and stronger hydrophobicity that inhibited the swelling and dissolution of hydrogels. On coming in contact with the MB solution, the CDPDPMcontaining hydrogel can adsorb MB and maintain the hydrogel state for recycling. On the contrary, the NaDC/Asp hydrogel dissolved and P(MEO 2 MA 90 -co-OEGMA 10 )-containing hydrogel collapsed in the MB solution. The properties of the hydrogels are expected to be tailored by introducing polymers with different properties, including the charge numbers, the number of available binding sites, and hydrophobic properties.

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“…1,2,3,4,5,6 Concerning hydrogels, in most of the cases they hierarchically self-assemble in aqueous media forming nanofibrous 3 dimensional structures. In this case, fibrillogenesis is based on the formation of β-sheet or α-helix secondary conformations of the polypeptide building blocks, mainly driven by hydrophobic interactions and stabilized by hydrogen bonding, developing fibrillar nanostructures of high aspect ratio.…”

Section: Introductionmentioning

confidence: 99%

Injectable Hydrogel: Amplifying the pH Sensitivity of a Triblock Copolypeptide by Conjugating the N-Termini via Dynamic Covalent Bonding

Popescu¹,

Liontos

Avgeropoulos

et al. 2016

ACS Appl. Mater. Interfaces

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We explore the self-assembly behavior of aqueous solutions of an amphiphilic, pH-sensitive poly(l-alanine)-b-poly(l-glutamic acid)-b-poly(l-alanine), (A5E11A5) triblock copolypeptide, end-capped by benzaldehyde through Schiff base reaction. At elevated concentrations and under physiological pH (7.4) and ionic strength (0.15M), the bare copolypeptide aqueous solutions underwent a sol-gel transition after heating and slow cooling thermal treatment, forming opaque stiff gels due to a hierarchical self-assembly that led to the formation of β-sheet-based twisted super fibers (Popescu et al. Soft Matter 2015, 11, 331-342). The conjugation of the N-termini with benzaldehyde (Bz) through a Schiff base reaction amplifies the copolypeptide pH-sensitivity within a narrow pH window relevant for in vivo applications. Specifically, the dynamic character of the imine bond allowed coupling/decoupling of the Bz upon switching pH. The presence of Bz conjugates to the N-termini of the copolypeptide resulted in enhanced packing of the elementary superfibers into thick and short piles, which inhibited the ability of the system for gelation. However, partial cleavage of Bz upon lowering pH to 6.5 prompted recovery of the hydrogel. The sol-gel transition triggered by pH was reversible, due to the coupling/decoupling of the benzoic-imine dynamic covalent bonding, endowing thus the gelling system with injectability. Undesirably, the gelation temperature window was significantly reduced, which however can be regulated at physiological temperatures by using a suitable mixture of the bare and the Bz-conjugated coplypeptide. This triblock copolypeptide gelator was investigated as a scaffold for the encapsulation of polymersome nanocarriers, loaded with a hydrophilic model drug, calcein. The polymersome/polypeptide complex system showed prolonged probe release in pH 6.5, which is relevant to extracellular tumor environment, rendering the system potentially useful for sustained delivery of anticancer drugs locally in the tumor.

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Peptide hydrogels

Cited by 289 publications

References 288 publications

Introduction to cell–hydrogel mechanosensing

Introduction to cell–hydrogel mechanosensing

Effects of polymers on the properties of hydrogels constructed using sodium deoxycholate and amino acid

Injectable Hydrogel: Amplifying the pH Sensitivity of a Triblock Copolypeptide by Conjugating the N-Termini via Dynamic Covalent Bonding

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