Effects of polymers on the properties of hydrogels constructed using sodium deoxycholate and amino acid

Abstract

The gelation behavior and properties of sodium deoxycholate (NaDC) and L-aspartic acid (Asp) in aqueous solution were investigated in detail at 25 C. The linear polymer poly(2-(2-methoxyethoxy)ethyl methacrylate-co-oligo-(ethylene glycol) methacrylate) (P(MEO 2 MA 90 -co-OEGMA 10 )) and star-shaped polymer poly(2-(dimethylamino)ethyl methacrylate-b-2-(2-methoxyethoxy)ethyl methacrylate) (CDPDPM) were introduced in NaDC/Asp hydrogels for exploring the effects of polymers on the properties of NaDC/Asp hydrogels and the mechanism underlying gelation processes by polymers was proposed. The hydrogels were characterized by phase behavior observation, polarized optical microscopy (POM), cryogenic scanning electron microscopy (cryo-SEM), X-ray powder diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy and rheological measurements. Moreover, the adsorption performances of hydrogels with and without polymers to methylene blue (MB) were studied using a UV-vis spectrometer. The results indicated that the transition from sol to gel state was observed with an increase in the Asp concentration in the system. Both linear and star-shaped polymers can participate in the formation of a gel network structure, so that the density of network structure and the mechanical strength of hydrogels increased. Furthermore, it was found that the viscoelasticity of the CDPDPM-containing hydrogel was much higher than that of the P(MEO 2 MA 90 -co-OEGMA 10 )-containing hydrogel under the same condition, indicating that CDPDPM performed better in strengthening the network structure of the hydrogels than P(MEO 2 MA 90 -co-OEGMA 10 ) due to the special structure that provided more binding sites for hydrogen bonding and stronger hydrophobicity that inhibited the swelling and dissolution of hydrogels. On coming in contact with the MB solution, the CDPDPMcontaining hydrogel can adsorb MB and maintain the hydrogel state for recycling. On the contrary, the NaDC/Asp hydrogel dissolved and P(MEO 2 MA 90 -co-OEGMA 10 )-containing hydrogel collapsed in the MB solution. The properties of the hydrogels are expected to be tailored by introducing polymers with different properties, including the charge numbers, the number of available binding sites, and hydrophobic properties.

Ionic Liquid-Polypyrrole-Gold Composites as Enhanced Enzyme Immobilization Platforms for Hydrogen Peroxide Sensing

“…As we all know the anionic surface active agent NaDC is usually used as gelatinizing agent for immobilizing biomolecules owing to its special rigid structure and good biocompatibility [39]. In the present study, NaDC was selected as the substitute of the ILs to prepare the NaDC-PPy/GCE and the corresponding conductivity was further studied.…”

Removal of Endotoxin from rAAV Samples Using a Simple Detergent-Based Protocol

“…It was earlier shown that cationic polymers and lipids can alter the infectivity of rAAV 19, 20, 21, 22. It is conceivable that more than a 20% transduction increase for rAAV5 after Na-DC treatment is caused by detergent residuals in the sample, especially given that Na-DC is known to form polymers 23 …”

Modified Low Molecular Weight Pure and Engineered Gels: A Review of Strategies towards Their Development

“…Participation of the polymers in the gelation process increased the density of gel network resulting in an increase in the mechanical strength of the hydrogel. [129] Besides using various external modifiers, researchers have also performed several of structural modifications in bile salts to alter the gelation properties. For example, Maitra and co-workers have reported the synthesis of bile salt derivatives through addition of some functional groups.…”

“…have studied the effect of a linear and a star shaped polymer on the morphological, optical, and rheological properties of NaDC‐L‐aspartic acid hydrogel. Participation of the polymers in the gelation process increased the density of gel network resulting in an increase in the mechanical strength of the hydrogel [129] . Besides using various external modifiers, researchers have also performed several of structural modifications in bile salts to alter the gelation properties.…”