Peptide Hydrogel Scaffold for Mesenchymal Precursor Cells Implanted to Injured Adult Rat Spinal Cord

Wiseman, Tylie M; Baron-Heeris, Danii; Houwers, Imke G. J.; Keenan, Rory; Williams, Richard J.; Nisbet, David R.; Harvey, Alan R.; Hodgetts, Stuart I.

doi:10.1089/ten.tea.2020.0115

Cited by 19 publications

(16 citation statements)

References 61 publications

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“…The significant variance in mechanical properties between peptide types is attributed to the mechanism of assembly driven by the residues in the sequence; 25,28 this indicates a peptide-specific relationship between the type of peptide and mechanics, which is important for the final application and tissue target. 37,73 This highlights the importance of this work, which seeks to retain the tissue-specific tunable bioactivity while determining an approach to enhance control over material properties and final utility.…”

Section: T H Imentioning

confidence: 99%

Tuneable Hybrid Hydrogels via Complementary Self-Assembly of a Bioactive Peptide with a Robust Polysaccharide

Firipis

Boyd‐Moss

Long

et al. 2021

ACS Biomater. Sci. Eng.

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Synthetic materials designed for improved biomimicry of the extracellular matrix must contain fibrous, bioactive, and mechanical cues. Self-assembly of low molecular weight gelator (LMWG) peptides Fmoc-DIKVAV (Fmoc-aspartic acid-isoleucine-lysine-valine-alanine-valine) and Fmoc-FRGDF (Fmoc-phenylalanine-arginine-glycine-aspartic acid-phenylalanine) creates fibrous and bioactive hydrogels. Polysaccharides such as agarose are biocompatible, degradable, and non-toxic. Agarose and these Fmoc-peptides have both demonstrated efficacy in vitro and in vivo. These materials have complementary properties; agarose has known mechanics in the physiological range but is inert and would benefit from bioactive and topographical cues found in the fibrous, protein-rich extracellular matrix. Fmoc-DIKVAV and Fmoc-FRGDF are synthetic self-assembling peptides that present bioactive cues “IKVAV” and “RGD” designed from the ECM proteins laminin and fibronectin. The work presented here demonstrates that the addition of agarose to Fmoc-DIKVAV and Fmoc-FRGDF results in physical characteristics that are dependent on agarose concentration. The networks are peptide-dominated at low agarose concentrations, and agarose-dominated at high agarose concentrations, resulting in distinct changes in structural morphology. Interestingly, at mid-range agarose concentration, a hybrid network is formed with structural similarities to both peptide and agarose systems, demonstrating reinforced mechanical properties. Bioactive-LMWG polysaccharide hydrogels demonstrate controllable microenvironmental properties, providing the ability for tissue-specific biomaterial design for tissue engineering and 3D cell culture.

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Section: T H Imentioning

confidence: 99%

Tuneable Hybrid Hydrogels via Complementary Self-Assembly of a Bioactive Peptide with a Robust Polysaccharide

Firipis

Boyd‐Moss

Long

et al. 2021

ACS Biomater. Sci. Eng.

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“…These IEIK13 implants (loaded with or devoid of chondrocytes) showed the restoration of full-thickness cartilage injuries, similar to cartilage-characteristic regeneration. Wiseman et al studied the ability of Fmoc-DIKVAV as scaffold and vehicle for grafted cells (in adult Fischer 344 (F344) rats) after mild thoracic contusion spinal cord injury (SCI) (thoracic level 10 [T10]) [ 149 ]. Their results showed that, after contusion SCI, Fmoc-DIKVAV hydrogel scaffold facilitated the cellular infiltration and axonal regrowth.…”

Section: Biomedical Applications Of Peptide-based Hydrogelsmentioning

confidence: 99%

Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications

et al. 2022

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Peptide-based hydrogels have attracted increasing attention for biological applications and diagnostic research due to their impressive features including biocompatibility and biodegradability, injectability, mechanical stability, high water absorption capacity, and tissue-like elasticity. The aim of this review will be to present an updated report on the advancement of peptide-based hydrogels research activity in recent years in the field of anticancer drug delivery, antimicrobial and wound healing materials, 3D bioprinting and tissue engineering, and vaccines. Additionally, the biosensing applications of this key group of hydrogels will be discussed mainly focusing the attention on cancer detection.

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“…For example, the delivery of pluripotent stem cells after dental root canal extraction or for a long-acting injectable implant where the introduction of infection is a risk and may wish to be prevented (ocular and spinal implants). [145][146][147] The potential for the peptide sequence to be modified in order to respond to infection or infectious stimuli (e.g. specific enzymes or pH change) is of major importance to the development of future technologies in this area.…”

Section: Future Perspectivesmentioning

confidence: 99%

Unravelling the antimicrobial activity of peptide hydrogel systems: current and future perspectives

et al. 2021

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Peptide Hydrogel Scaffold for Mesenchymal Precursor Cells Implanted to Injured Adult Rat Spinal Cord

Cited by 19 publications

References 61 publications

Tuneable Hybrid Hydrogels via Complementary Self-Assembly of a Bioactive Peptide with a Robust Polysaccharide

Tuneable Hybrid Hydrogels via Complementary Self-Assembly of a Bioactive Peptide with a Robust Polysaccharide

Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications

Unravelling the antimicrobial activity of peptide hydrogel systems: current and future perspectives

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