Peptide functionalized poly(l-lysine)-g-poly(ethylene glycol) on titanium: resistance to protein adsorption in full heparinized human blood plasma

Tosatti, Samuele; Paul, Susan M. De; Askendal, Agneta; VandeVondele, Stephanie; Hubbell, Jeffrey A.; Tengvall, Pentti; Textor, Marcus

doi:10.1016/s0142-9612(03)00420-4

Cited by 193 publications

(209 citation statements)

References 34 publications

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“…Poly(ethylene glycol) (PEG) has been reported to have the ability to inhibit protein adhesion, platelet adhesion, bacterial adhesion, or biofilm formation 14,15) . In addition, PEG-immobilized Ti has been demonstrated to be effective in suppressing biofilm formation, protein adsorption, and bacterial adhesion [16][17][18] . In these studies, the methods used to immobilize PEG on metal surfaces have been complicated by the inclusion of a multistage process for the synthesis of poly( L -lysine) and PEG (PLL-g-PEG) graft copolymers and stabilization by immersion.…”

Section: Fig 1 Schematic Illustration Of Peg Electrodeposited Onmentioning

confidence: 99%

Evaluation of biofilm formation in the presence of saliva on poly(ethylene glycol)deposited titanium

Kawabe¹,

Nakagawa²,

Kanno³

et al. 2014

Dent. Mater. J.

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Titanium (Ti) is widely used for oral cavity biomedical devices. However, because it penetrates the mucosa and exists partially external to the tissue, it sometimes induces tissue inflammation, minor infection, or peri-implantitis due to oral bacteria after implantation and causes serious consequences. We have previously shown that poly(ethylene glycol) (PEG)-electrodeposited Ti inhibits bacterial adhesion and biofilm formation. However, the effect of the PEG coating in body fluid is still unclear. In this study, we investigated bacterial colony morphology and biofilm formation on PEG-electrodeposited Ti in comparison with untreated Ti in the presence of saliva. After 48 h incubation, Streptococcus mutans biofilms adhered on the untreated Ti were rigid and cohesive, while those on the PEG-electrodeposited were loose and were easily washed off. These results indicate electrodeposited-PEG layers inhibit the biofilm formation on Ti in the presence of saliva.

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Section: Fig 1 Schematic Illustration Of Peg Electrodeposited Onmentioning

confidence: 99%

Evaluation of biofilm formation in the presence of saliva on poly(ethylene glycol)deposited titanium

Kawabe¹,

Nakagawa²,

Kanno³

et al. 2014

Dent. Mater. J.

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“…Coatings based on human protein such as albumin or human serum have been shown to reduce S. aureus and S. epidermidis adhesion to the surface (Kinnari et al, 2005). Poly(l-lysine)-grafted-poly(ethylene glycol) (PLL-g-PEG) coatings have been extensively studied for use in biomedical applications, and are highly effective in reducing the adsorption of blood serum, blood plasma and single proteins, such as fibrinogen and albumin (Tosatti et al, 2003). It is also known that fibroblast and osteoblast cell adhesion and spreading on metal oxide surfaces coated with PLL-g-PEG is strongly reduced in comparison to uncoated oxide surfaces .There has also been interest in coating osteosynthesis implants (stainless steel, titanium, or titanium alloy) with a thin layer of antibiotic-loaded biocompatible, biodegradable polymer, such as polylactic-co-glycolic acid (PLGA) (Price et al, 1996), and poly(D,L-lactide) (PDLLA) (Gollwitzer et al, 2003).…”

Section: Implanted Materials and Osteomyelitismentioning

confidence: 99%

Pathophysiology and Pathogenesis of Osteomyelitis

Roy¹,

Somerson²,

Kerr³

et al. 2012

Osteomyelitis

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“…Two of the most common bulk techniques are: making the surface more hydrophilic through a variety of processes such as plasma etching [6,[20][21][22][23]; or modifying the surface with a hydrophilic polymer such as polyethylene glycol [24][25][26][27][28][29][30]. Other methodologies have used smallscale modifications at the micro-and nanolevels to create patterned surfaces that can regulate protein and cellular behaviour [31][32][33][34][35][36].…”

Section: (B) Engineering Surfaces To Control Protein Adsorptionmentioning

confidence: 99%

Designing nanostructured block copolymer surfaces to control protein adhesion

Schricker

Palacio

Bhushan

2012

Phil. Trans. R. Soc. A.

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The profile and conformation of proteins that are adsorbed onto a polymeric biomaterial surface have a profound effect on its in vivo performance. Cells and tissue recognize the protein layer rather than directly interact with the surface. The chemistry and morphology of a polymer surface will govern the protein behaviour. So, by controlling the polymer surface, the biocompatibility can be regulated. Nanoscale surface features are known to affect the protein behaviour, and in this overview the nanostructure of self-assembled block copolymers will be harnessed to control protein behaviour. The nanostructure of a block copolymer can be controlled by manipulating the chemistry and arrangement of the blocks. Random, A-B and A-B-A block copolymers composed of methyl methacrylate copolymerized with either acrylic acid or 2-hydroxyethyl methacrylate will be explored. Using atomic force microscopy (AFM), the surface morphology of these block copolymers will be characterized. Further, AFM tips functionalized with proteins will measure the adhesion of that particular protein to polymer surfaces. In this manner, the influence of block copolymer morphology on protein adhesion can be measured. AFM tips functionalized with antibodies to fibronectin will determine how the surfaces will affect the conformation of fibronectin, an important parameter in evaluating surface biocompatibility.

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Peptide functionalized poly(l-lysine)-g-poly(ethylene glycol) on titanium: resistance to protein adsorption in full heparinized human blood plasma

Cited by 193 publications

References 34 publications

Evaluation of biofilm formation in the presence of saliva on poly(ethylene glycol)deposited titanium

Evaluation of biofilm formation in the presence of saliva on poly(ethylene glycol)deposited titanium

Pathophysiology and Pathogenesis of Osteomyelitis

Designing nanostructured block copolymer surfaces to control protein adhesion

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