Peptide-Functionalized Nanoparticles-Encapsulated Cyclin-Dependent Kinases Inhibitor Seliciclib in Transferrin Receptor Overexpressed Cancer Cells

He, Guan Zhen; Lin, Wen Jen

doi:10.3390/nano11030772

Cited by 21 publications

(12 citation statements)

References 32 publications

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“…The higher expression of Tf receptors on U87 cells enables a much greater proportion of targeted niosomes to enter the cells via receptor-mediated endocytosis, in comparison to non-targeted niosomes. We verified this Tf-receptor-mediated endocytosis of Tf-functionalized PEGNIO formulations by Tf-receptor-expressing U87 glioma cells via performing flow-cytometric cellular uptake measurements of fluorescence-labeled PEGNIO/Tf in Tf-receptor negative cells (A549, which expresses hardly any Tf-receptors [ 49 ]) ( Figure S7 ). Because, in this case, the internalization of PEGNIO and PEGNIO/Tf was shown to be almost uniform and therefore independent of Tf-functionalization, the presented enhanced internalization of Tf-labeled PEGNIO/QDs/MIONs by U87 cells can be attributed to a Tf-based endocytosis.…”

Section: Resultsmentioning

confidence: 83%

Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma

Şeleci

Maurer

Barlas

et al. 2021

IJMS

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The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEGNIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.

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Section: Resultsmentioning

confidence: 83%

Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma

Şeleci

Maurer

Barlas

et al. 2021

IJMS

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“…Cell Cultures. Human breast cancer cells MDA-MB-231 (TfR1 highly overexpressed) and lung carcinoma cells A549 (TfR1 expressed, lower level than MDA-MB-231 cells) 32 were provided by the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% (v/v) FBS and 1% penicillin−streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

ERK-Peptide-Inhibitor-Modified Ferritin Enhanced the Therapeutic Effects of Paclitaxel in Cancer Cells and Spheroids

Dong

et al. 2021

Mol. Pharmaceutics

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Rational design of a drug delivery system with enhanced therapeutic potency is critical for efficient tumor chemotherapy. Many protein-based drug delivery platforms have been designed to deliver drugs to target sites and improve the therapeutic efficacy. In this study, paclitaxel (PTX) molecules were encapsulated within an apoferritin nanocage-based drug delivery system with the modification of an extracellular-signal-regulated kinase (ERK) peptide inhibitor at the C-terminus of ferritin (HERK). Apoferritin is an endogenous nano-sized spherical protein which has the ability to specially bind to a majority of tumor cells via interacting with transferrin receptor 1. The ERK peptide inhibitor is a peptide which can disrupt the interaction of MEK with ERK in the mitogen-activated protein kinase/ERK pathway. By combining the targeted delivery effect of ferritin and the inhibitory effect of the ERK peptide inhibitor, the newly fabricated ferritin carrier nanoparticle HERK could still be taken up by tumor cells, and it displayed higher cell cytotoxicity than the parent ferritin. After loading with PTX, HERK-PTX displayed a favorable anticancer effect in human breast cancer cells MDA-MB-231 and lung carcinoma cells A549. The remarkable inhibitory effect on MDA-MB-231 tumor spheroids was also identified. These results indicated that the constructed HERK nanocarrier is a promising multi-functional drug delivery vehicle to enhance the therapeutic effect of drugs in cancer therapy.

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“…Cellular targeting and uptake of the bioconjugates were also investigated using the MDA-MB-231 breast cancer cell line, which overexpresses the Tf receptor. 50 Most importantly, when investigated in a healthy mouse model, Tf-PMSEA 16.2k was able to significantly improve the in vivo pharmacokinetics and bioavailability of the native protein compared to Tf-POEGA 16.2k and native Tf due to its lowfouling nature. Moreover, when tested in mice bearing subcutaneous MDA-MB-231 breast tumors, a remarkably high tumor accumulation of Tf-PMSEA 16.2k was observed compared to the other groups, and hence the conjugate is expected to perform favorably for therapeutic delivery.…”

Section: ■ Introductionmentioning

confidence: 97%

Stimuli-Responsive Sulfoxide Polymer–Protein Conjugates with Improved Pharmacokinetics and Tumor Delivery

Ediriweera,

Chang,

Wang

et al. 2023

Chem. Mater.

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A growing number of reports of poly(ethylene glycol) (PEG)associated immunogenicity have prompted the development of alternative polymers for bioconjugation to assist in overcoming the poor in vivo pharmacokinetic profile of proteins. In this study, we demonstrate the development of transferrin (Tf) protein−polymer conjugates using a highly hydrophilic sulfoxide polymer (poly(2-(methylsulfinyl)ethyl acrylate) (PMSEA)) that has recently emerged as a promising class of low-fouling polymer. A cleavable thioketal linker between the protein and polymer enabled successful protein release when the conjugates were exposed to an environment rich in reactive oxygen species. Cleavage of the polymer circumvents the loss of protein activity upon chemical modification. The Tf-PMSEA conjugate was capable of significantly improving the in vivo pharmacokinetics of the protein and consequently enabled higher tumor accumulation of Tf-PMSEA in tumorbearing mice compared to the PEGylated counterpart. This demonstrated the potential of PMSEA to be utilized in therapeutic delivery applications as a promising alternative to PEG.

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Peptide-Functionalized Nanoparticles-Encapsulated Cyclin-Dependent Kinases Inhibitor Seliciclib in Transferrin Receptor Overexpressed Cancer Cells

Cited by 21 publications

References 32 publications

Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma

Transferrin-Decorated Niosomes with Integrated InP/ZnS Quantum Dots and Magnetic Iron Oxide Nanoparticles: Dual Targeting and Imaging of Glioma

ERK-Peptide-Inhibitor-Modified Ferritin Enhanced the Therapeutic Effects of Paclitaxel in Cancer Cells and Spheroids

Stimuli-Responsive Sulfoxide Polymer–Protein Conjugates with Improved Pharmacokinetics and Tumor Delivery

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