Peptide-crosslinked molecularly imprinted polymers for efficient separation of immunoglobulin G from human serum

Dong, Qiujing; Mu, Yang; Wang, Yafei; Guan, Ying; Zhang, Wangqing; Zhang, Yongjun

doi:10.1039/d2bm01450e

Cited by 8 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the shape of the imprint cavities could be largely restored. Since both the original size and shape of the imprint cavities are restored, the MIP exhibits high affinity to the template protein. − …”

Section: Results and Discussionmentioning

confidence: 99%

“…Herein, a novel hemoglobin-imprinted polymer was designed using a recently developed strategy. − In this strategy, the aforementioned problems are overcome simultaneously via the construction of shape-memorable imprint cavities. When removing the template protein, the cavities are enlarged by pH change to allow the complete release of the template.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecularly Imprinted Polymers with Shape-Memorable Imprint Cavities for Efficient Separation of Hemoglobin from Blood

Dong

Guan

et al. 2023

Biomacromolecules

Self Cite

View full text Add to dashboard Cite

Efficient separation and purification of hemoglobin from blood and other complicated biological fluids still remains a big challenge. Molecularly imprinted polymers (MIPs) of hemoglobin are potential choices; however, they suffer from severe problems including difficult template removal and low imprinting efficiency like other protein-imprinted polymers. Herein, a novel MIP of bovine hemoglobin (BHb) was designed in which a peptide crosslinker (PC), instead of the commonly used crosslinkers, was used. The PC, a random copolymer of lysine and alanine, adopts an α-helical conformation at pH 10 but transits to a random coil conformation at pH 5. The introduction of alanine residues lowers the pH range at which the PC undergoes helix-coil transition. The imprint cavities in the polymers are shape-memorable due to the reversible and precise helix-coil transition of the peptide segments in the polymers. They can be enlarged by lowering pH from 10 to 5, thus allowing complete removal of the template protein under mild conditions. When the pH is adjusted back to 10, their original size and shape will be recovered. Therefore, the MIP binds the template protein BHb with high affinity. Compared with the MIP crosslinked with the commonly used crosslinker, the imprinting efficiency of the PC-crosslinked MIP is significantly improved. In addition, both the maximum adsorption capacity (641.9 mg/g) and imprinting factor (7.2) are much higher than the BHb MIPs reported previously. The new BHb MIP also exhibits high selectivity toward BHb and good reusability. Thanks to the high adsorption capacity and high selectivity of the MIP, when it was applied to extract BHb from bovine blood, BHb in the blood sample was extracted almost completely, and high purity product was obtained.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymers with Shape-Memorable Imprint Cavities for Efficient Separation of Hemoglobin from Blood

Dong

Guan

et al. 2023

Biomacromolecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…Instead of the commonly used cross-linkers, such as BIS, this strategy uses peptide cross-linkers to synthesize the polymers. These peptide cross-linkers can be synthesized by solid phase peptide synthesis − or ring-opening polymerization of NCAs. − Unlike solid phase peptide synthesis, which is a stepwise procedure and therefore laborious and time-consuming, ring-opening polymerization of NCAs is one-step and allows for massive production. However, the two vinyl groups of the previously synthesized peptide cross-linkers are different and therefore may have different reactivities .…”

Section: Resultsmentioning

confidence: 99%

“…The peptide chains were introduced by using peptide cross-linkers (PCs). Previously we synthesized the PCs via solid phase peptide synthesis. − This stepwise and therefore laborious and time-consuming procedure was later replaced by one-step ring-opening polymerization of N-carboxyanhydrides (NCAs). − However, the two vinyl groups of the peptide cross-linkers are different, and these will have different reactivities. Here a PC with the same vinyl groups on both ends was synthesized.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymer with High Capacity and Selectivity for Separation of Bovine Serum Albumin

Zhang,

Wang,

et al. 2023

ACS Appl. Polym. Mater.

Self Cite

View full text Add to dashboard Cite

A cost-effective but simple method for the separation of bovine serum albumin (BSA) from complex biological fluids is still lacking. Here using N-isopropylacrylamide as the main monomer and dimethylaminoethyl methacrylate, methacrylic acid, and acrylamide as functional monomers, a BSA-imprinted polymer was synthesized. To overcome the two major obstacles of common protein-imprinted polymers, i.e., difficult template removal and low imprinting efficiency, an oligo(glutamic acid)-based peptide cross-linker with the same vinyl group on both ends was designed and used as cross-linker in the synthesis of the polymers. The introduction of the peptide segments, which are capable of undergoing a pH-triggered reversible and precise helix−coil transition, into the polymers makes the imprint cavities shape-memorable. Therefore, complete removal of the template BSA under mild conditions, that is, at physiological pH, ionic strength, and temperature, was achieved. More importantly, an adsorption capacity of 607 mg/g and an imprinting factor of 6.0, both of which are not only higher than the molecularly imprinted polymer (MIP) cross-linked with the commonly used cross-linker N,N′-methylenebis(acrylamide), but also higher than most BSA MIPs reported previously in the literature, were obtained, indicating a high affinity of the MIP to the template BSA. The BSA MIP also showed a high selectivity and could be used repeatedly. Using the BSA MIP as the adsorbent, BSA was extracted from fetal bovine serum selectively and efficiently.

show abstract

“…Whereas molecular imprinting of polymers only requires a high-purity sample of the target molecule and allows for the relatively straightforward synthesis of equally specific targeting materials at a lower cost [ 17 , 18 ]. MIPs are based on the polymerization of a specific monomer and crosslinking agent in the presence of a template [ 19 ]. This molding process produces a specific structure that is selective to the molecule of interest used as a template.…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

et al. 2023

View full text Add to dashboard Cite

Cancer is a disease caused by abnormal cell growth that spreads through other parts of the body and threatens life by destroying healthy tissues. Therefore, numerous techniques have been employed not only to diagnose and monitor the progress of cancer in a precise manner but also to develop appropriate therapeutic agents with enhanced efficacy and safety profiles. In this regard, molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. Taken together, the topics discussed in this review provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment. Graphical Abstract Molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for cancer theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. The topics discussed in this review aim to provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment.

show abstract

Peptide-crosslinked molecularly imprinted polymers for efficient separation of immunoglobulin G from human serum

Abstract: Low-cost and highly effective methods are highly desirable to replace the costly ethanol fractionation and aﬃnity chromatography in IgG isolation from human plasma. Molecularly imprinted polymers (MIPs) of IgG are...

Cited by 8 publications

References 40 publications

Molecularly Imprinted Polymers with Shape-Memorable Imprint Cavities for Efficient Separation of Hemoglobin from Blood

Molecularly Imprinted Polymers with Shape-Memorable Imprint Cavities for Efficient Separation of Hemoglobin from Blood

Molecularly Imprinted Polymer with High Capacity and Selectivity for Separation of Bovine Serum Albumin

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

Contact Info

Product

Resources

About