1996
DOI: 10.1002/eji.1830260836
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Peptide binding specificity of major histocompatibility complex class I resolved into an array of apparently independent subspecificities: quantitation by peptide libraries and improved prediction of binding

Abstract: Considerable interest has focused on understanding how major histocompatibility complex (MHC) specificity is generated and characterizing the specificity of MHC molecules with the ultimate goal being to predict peptide binding. We have used a strategy where all possible peptides of a particular size are distributed into positional scanning combinatorial peptide libraries (PSCPL) to develop a highly efficient, universal and unbiased approach to address MHC specificity. The PSCPL approach appeared qualitatively … Show more

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Cited by 90 publications
(83 citation statements)
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“…The peptide binding specificity of a given HLA-I is determined by favoring or disfavoring of certain amino acids in certain positions of the peptide. An unbiased method has previously been reported capable of determining the peptide binding specificities of HLA-I molecules using PSCPLs (17). To investigate whether Tpn 1-87 alters or shows a preference for facilitation based on the peptide binding specificity of HLA-A*02:01, i.e.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The peptide binding specificity of a given HLA-I is determined by favoring or disfavoring of certain amino acids in certain positions of the peptide. An unbiased method has previously been reported capable of determining the peptide binding specificities of HLA-I molecules using PSCPLs (17). To investigate whether Tpn 1-87 alters or shows a preference for facilitation based on the peptide binding specificity of HLA-A*02:01, i.e.…”
Section: Resultsmentioning
confidence: 99%
“…We used a previously reported method capable of determining the HLA-I peptide binding specificity using PSCPLs (17). In brief, peptides are synthesized with completely random amino acids at all positions in the peptide except at a chosen position.…”
Section: Methodsmentioning
confidence: 99%
“…Prediction matrices of Parker et al [26] were generated from a limited set of peptides, perhaps explaining a recent report [33] describing poor correspondence between the predicted MHC binding peptides and those determined experimentally. Quantitative matrices have also been derived from positional scanning combinatorial peptide libraries (PSCPL) [27,28], where all possible peptides of a given length are represented by sets of sublibraries and in each sublibrary, one amino acid is kept fixed whereas the remaining positions contain mixtures of all amino acids. Unfortunately, to date, prediction of pMHCI binding using these PSCPL-derived matrices is not freely accessible.…”
Section: Discussionmentioning
confidence: 99%
“…Synthesized peptides were purified by reverse-phase HPLC to .80% purity and validated by mass spectrometry. Positional scanning combinatorial peptide libraries (PSCPL) were synthesized as previously described (17). Briefly, eight of nine positions comprised an equimolar pool of 19 of the 20 natural amino acids (i.e., excluding cysteine), whereas the remaining position comprised 1 of the 20 natural amino acids (i.e., including cysteine), thereby interrogating the position-specific effect of this latter amino acid.…”
Section: Peptidesmentioning
confidence: 99%
“…Determining peptide-binding motifs by a scintillation proximity-based dissociation assay Nonameric peptide-binding motifs were determined for HLA-C*01:02, -C*02:02, -C*03:02, -C*03:03, -C*03:04, -C*04:01, -C*05:01, -C*06:02, -C*07:01, -C*07:02, -C*07:04, -C*08:01, -C*08:02, -C*12:03, -C*14:02, -C*15:02, and -C*16:01 using a recently developed scintillation proximity assay (SPA)-based peptide-MHC-I dissociation assay (23) in combination with nonameric PSCPL (17). In 384-well streptavidin-coated FlashPlate HTS Plus microplates (PerkinElmer, SMP410), recombinant biotinylated MHC-I H chain in 8 M urea was diluted at least 100 times (to 50 nM) into PBS/0.1% Lutrol F68 containing 78 mg/ml (78 mM) of each sublibrary with 2-10 nM [ …”
Section: Radiolabelingmentioning
confidence: 99%