Peptide behaviour and analysis on a chemically stable C18-bonded vinyl alcohol copolymer column with alkaline and acidic eluents

Serotonin uptake stimulating peptide found in plasma of normal individuals and in some autistic urines

“…The only fit possible would be amides of pE‐G‐W or pE‐W‐G because of the level of UV 280 absorbtion. The elution point also agreed with the theoretically calculated one for pE‐W‐GNH 2 on reverse phase HPLC (14, 15). Hydrolysed peptide was inactive (Fig.…”

Review on the chemical and thermal stability of stationary phases for reversed-phase liquid chromatography

“…In summary, several studies have discussed static emerging and also dynamic purging of RP columns under recycling as well as non-recycling conditions [72,73,76,[78][79][80][81]. Due to saturation effects of stationary phase deterioration products in the eluent, especially chemical stability results from static or recycling eluent experiments must be compared with caution.…”

“…In several studies the chemical and temperature stability of polymer-based RPLC phases was studied. Uchida investigated the chemical stability of a C-18 bonded vinyl alcohol polymer [79]. Based on static immersion experiments, the authors found an excellent chemical stability of that specific polymer phase in the pH range 2-13 during one-half to 2 days at a temperature of 50 • C. However, the long-term chemical stability for this particular phase is difficult to judge because, as discussed earlier in Section 2.1.1, several different approaches to determine the chemical stability of RP phases hamper a fair comparison of the data from different studies.…”

High-performance liquid chromatography (advances in packing materials)

“…The ranges of pH stability are stated to be 1-13 for PS-DVB packing materials and 2-12 for HEMA (039) and other PHAM (038) packing materials. A Ci8 PVA packing material (Asahipak ODP-50) was immersed for 48 h in 50 mM sodium phosphate/acetonitrile (90:10) at pHs 2,10, and 13 at 50 °C, showing no weight or efficiency loss at pHs 2 and 10, while showing a 1% decrease in weight and a 12% loss in retention time (calculated from a figure in the paper) at pH 13 (040). The manufacturer's catalog rates the pH stability of its PVA packing materials to be from either 2 to 9 or 2 to 12 for size-exclusion, 2 to 13 for reversed-phase, and 2 to 12 for ion-exchange packing materials ('91-'92 Technical Data Compendium, Asahi Chemical).…”