Peptide-based simple detection of SARS-CoV-2 with electrochemical readout

Electrochemical genosensor for the specific detection of SARS-CoV-2

“…The higher HRP molecules of streptavidin poly-HRP80 concerning the streptavidin poly-HRP20 enzymatic complex expect the highest electrochemical biosensing signal response [ 44 ]; however, the biosensing response was quite comparable in both cases. We hypothesized that having a complex with a higher number of molecules as a signaling tag may induce steric repulsion, limiting the contact of streptavidin poly-HRP80 complex with the biotinylated signaling probes, leading to a slightly reduced biosensing response to streptavidin-HRP20.…”

“…Other few authors have also reported electrochemical biosensors for N gene detection [ 32 ] for the detection of spike protein [ [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] ], immunoglobulins IgG and IgM [ 15 ], and pseudotyped viral particles [ 41 , 42 ]. An immunosensor [ 43 ] and a peptide-based biosensor [ 44 ] were recently reported by our group to rapidly detect the spike protein and the viral particles in clinical samples with simple electrochemical formats and values correlated with the RT-PCR standard method.…”

An extracellular matrix biosensing mimetic for evaluating cathepsin as a host target for COVID-19

“…Peptide probe 1, for cathepsin B (11-Mercaptoundecanoic acid (MUA)K-DAHKI-(ARKKAAKA) 3 VLVLVLVL), and probe 2, for TGF beta 1 ((MUA)K–FSHF-(ARKKAAKA) 3 VLVLVLVL), were custom-synthesized as lyophilized powder, purity>95%, by Shanghai Science Peptide Co, Ltd. Cathepsin B, TGF beta 1 and anti-TGF beta 1 antibody as its inhibitor, were from R&D system. MUA, 9-mercapto-1-nonanol (MN), benzylviologen (BV) and cucurbit [ 8 ]uril (CB [ 8 ]) were from Sigma-Aldrich. All the other chemicals were of analytical grade.…”

“…The solutions of the peptide probe were prepared by dissolving the powder into 10 μM with 10 mM phosphate buffer solution (PBS) (pH 7.4). The label in Scheme 1 b was prepared as follows: Briefly, BV and CB [ 8 ] powder of equal molar amounts were dissolved in 10 mM PBS (pH 7.0) to 750 μM followed by moderate vortex-agitating overnight. This solution was freshly prepared each time and was brought to its experimental application immediately after the agitation process.…”

“…The complete image of the interactions can only be pieced together by combing the results of several separated assays. Recently, with the development of biosensing and interface nanotechnology [ 8 , 9 ], it is now possible to construct biosensing interfaces mimicking the ECM. In such a biomimetic sensing system, the secreted bioactive factors could interact with the sensing interface in similar ways as with the ECM, resulting in analogous biophysical and biological changes in the biosensing interface.…”