Peptide-Based Sandwich Immunoassay for the Quantification of the Membrane Transporter Multidrug Resistance Protein 1

Poetz, Oliver; Dieze, Theresa; Hammer, Helen; Weiss, F. T.; Sommersdorf, Cornelia; Templin, Markus F.; Esdar, Christina; Zimmermann, Astrid; Stevanović, Stefan; Bedke, Jens; Stenzl, Arnulf; Joos, Thomas

doi:10.1021/acs.analchem.8b00152

Cited by 6 publications

(3 citation statements)

References 32 publications

(55 reference statements)

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“…Single-analyte sandwich immunoassays were also performed for MCSF1R and OPN using antibodies and calibrators from commercially available kits (R&D, Minneapolis, Canada: OPN: DuoSet #DY1433, MCSF1R: DuoSet #DY329) adapted to the Luminex bead-based assay platform (Luminex xMAP, Luminex Corp., Austin, USA) according to the workflows described in detail elsewhere. , Briefly, the capture antibodies were coupled to magnetic polystyrene beads and incubated with a calibrator, controls, or unknown samples for 2 h (sample dilutions: MCSF1R: 1:100, OPN: 1:10). Thereafter, samples were incubated with the biotinylated detection antibodies for 2 h followed by another incubation with phycoerythrin (PE)-conjugated streptavidin for 45 min.…”

Section: Methodssupporting

confidence: 91%

Matrix and Sampling Effects on Quantification of Protein Biomarkers of Drug-Induced Liver Injury

Anselm¹,

Sommersdorf²,

Carrasco‐Triguero³

et al. 2021

J. Proteome Res.

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Macrophage colony stimulating factor 1 receptor (MCSF1R), osteopontin (OPN), high-mobility group protein B1 (HMGB1), glutamate dehydrogenase (GLDH), keratin 18 (K18), and caspase-cleaved keratin 18 (ccK18) are considered promising mechanistic biomarkers for the diagnosis of drug-induced liver injury. Here, we aim to elucidate the impact of the sample matrix and handling on the quantification of these emerging protein biomarkers. We investigated effects such as time from collection to centrifugation during serum (± gel) or EDTA plasma preparation on two assay platforms: immunoaffinity liquid chromatography mass spectrometric assays and sandwich immunoassays. Furthermore, we measured GLDH activity with an enzymatic activity assay. Matrix effects were observed particularly for HMGB1 and MCSF1R. HMGB1 levels were higher in serum than in plasma, whereas higher concentrations of MCSF1R were observed in plasma than in serum. A comparison of sample collection to centrifugation time ranging from 15 to 60 min demonstrated increasing levels of HMGB1 in serum, while MCSF1R, OPN, GLDH, and ccK18 concentrations remained stable. Additionally, there was a poor correlation in HMGB1 and ccK18 levels between serum and plasma. Considering the observed matrix effects, we recommend plasma as a matrix of choice and cross-study comparison studies to be limited to those using the same matrix.

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Section: Methodssupporting

confidence: 91%

Matrix and Sampling Effects on Quantification of Protein Biomarkers of Drug-Induced Liver Injury

Anselm¹,

Sommersdorf²,

Carrasco‐Triguero³

et al. 2021

J. Proteome Res.

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“…mAbs are produced from a single clone of pure and specific cells or cell line, and consist of identical antibodies. Until now, mAbs have been used to detect almost every target class, including metal ions, [ 26 ] mycotoxins, [ 27 ] pesticides, [ 28 ] veterinary drugs, [ 29 ] nucleic acids, [ 30 ] proteins, [ 31 ] viruses, [ 32 ] and microorganisms. [ 33 ]…”

Section: Biomolecules Used In Lateral Flow Assaysmentioning

confidence: 99%

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Wang

Zhao

et al. 2021

Small Methods

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The development of the lateral flow assay (LFA) has received much attention in both academia and industry because of their broad applications to food safety, environmental monitoring, clinical diagnosis, and so forth. The user friendliness, low cost, and easy operation are the most attractive advantages of the LFA. In recent years, quantitative detection has become another focus of LFA development. Here, the most recent studies of quantitative LFAs are reviewed. First, the principles and corresponding formats of quantitative LFAs are introduced. In the biomaterial and nanomaterial sections, the detection, capture, and signal amplification biomolecules and the optical, fluorescent, luminescent, and magnetic labels used in LFAs are described. The invention of dedicated strip readers has drawn further interest in exploiting the better performance of LFAs. Therefore, next, the development of dedicated reader devices is described and the usefulness and specifications of these devices for LFAs are discussed. Finally, the applications of LFAs in the detection of metal ions, biotoxins, pathogenic microorganisms, veterinary drugs, and pesticides in the fields of food safety and environmental health and the detection of nucleic acids, biomarkers, and viruses in clinical analyses are summarized.

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“…Highly selective and efficient recognition and localization are critical for both detection and cure of cancers. − Due to the lack of specificity and cancer affinity, traditional drugs are usually accompanied by severe side effects, low absorption in tumor sites and drug resistance, leading to the failure in the treatment. − To minimize systemic toxicity, drugs are given at suboptimal dosages, which however results in limited clinical efficacy . Multidrug resistance (MDR), as another obstacle for chemotherapeutics, can render drugs ineffective via different mechanisms, including reducing cellular uptake of drugs. ,− Discovery of novel approaches for simultaneously reducing side effect and bypassing drug resistance are highly desired for effectively probing cancers, however still remain a challenging task.…”

mentioning

confidence: 99%

Peptide-Guided System with Programmable Subcellular Translocation for Targeted Therapy and Bypassing Multidrug Resistance

et al. 2018

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Nonselectivity and drug resistance are two major obstacles for cancer treatment. Although great advances have been made toward cell targeting or discovering novel delivery pathways, it is still desirable to simultaneously overcome the two hurdles for successful cancer theranostics. Herein, a peptide-guided system was tailored by modular integration of a cancer biomarker-specific peptide, a mitochondria-targeting motif and a cell toxin. Cell imaging analysis revealed that the dual-targeting peptide-drug conjugate (PDC) features in cancer cell-specific uptake, strong drug retention, and programmable intracellular translocation. Facilitated by in situ bond cleavage, PDC successfully diverted the toxic effect of nucleus-localized drug to mitochondria. Mechanism investigation demonstrated that the cell damage pathway of the drug was also transformed, which is beneficial to reverse drug resistance in cancer cells. The effectiveness of PDC for cancer therapy was further demonstrated by in vivo imaging and tumor inhibition assay. With intravenous injection, targeted accumulation in the tumor site, and tumor suppressing efficacy without side effects exhibit its perspective for cancer treatment. The dual-targeting peptidedrug conjugate featuring tailored transportation route highlights a promising and generally applicable way to enhance the overall therapeutic index of conventional anticancer drugs.

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Peptide-Based Sandwich Immunoassay for the Quantification of the Membrane Transporter Multidrug Resistance Protein 1

Cited by 6 publications

References 32 publications

Matrix and Sampling Effects on Quantification of Protein Biomarkers of Drug-Induced Liver Injury

Matrix and Sampling Effects on Quantification of Protein Biomarkers of Drug-Induced Liver Injury

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Peptide-Guided System with Programmable Subcellular Translocation for Targeted Therapy and Bypassing Multidrug Resistance

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