Peptidase compensation in the digestive system of whiteleg shrimp <i>Penaeus vannamei</i>
 against dietary Kunitz-type soybean trypsin inhibitor

Maytorena-Verdugo, Claudia I.; Córdova-Murueta, Julio H.; García‐Carreño, Fernando

doi:10.1111/anu.12477

Cited by 11 publications

(4 citation statements)

References 45 publications

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“…When ingested by shrimp, the TI will form competitive bindings that reduce proteolytic activity and chymotrypsin enzyme activity by 65%. The existence of TI in the shrimp gastrointestinal tract from 2 to 4 hours also inhibited 40% of trypsin enzyme activity (Maytorena-Verdugo et al, 2017). However, trypsin inhibitors are highly stable and difficult to degrade during heat treatment to create animal feed.…”

Section: Introductionmentioning

confidence: 99%

The growth and probiotic characteristics of Bacillus velezensis BS in soybean meal used as synbiotic-like preparations for Litopenaeus vannamei culture

Hoang,

Nguyen,

Chu

et al. 2023

Vietnam J. Biotechnol.

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Soybean meal (SBM) is the by-product of soybean oil extraction. It is well known to contain at least 47% protein and more than 6% crude fiber. This study showed that Bacillus velezensis BS grew well in medium containing 1% SBM and gained the prebiotic index and activity score of 15.40 ± 4.10 and 0.85 ± 0.22, respectively. In this medium, B. velezensis BS showed various probiotic characteristics, such as digestive enzyme secretion (α-amylase, cellulase, and protease), anti-Vibrio parahaemolyticus, biofilm formation on the solid surface after 24 h of interaction and the ability to grow in a broad range of environmental conditions (pH 6-9, temperature of 20-45ºC, salinity of 4%). In addition, SBM fermented with B. velezensis BS significantly reduced 79.7 ± 1.9% of the trypsin inhibitor content. Dietary supplement of the fermented product in Litopenaeus vannamei culture improved the specific growth rate of shrimp by approximately 7 times and concomitantly decreased the density of V. parahaemolitycus pathogen by 10 times compared to the group without synbiotic-like fed. Hence, the B. velezensis BS and its SBM fermented product were suggested as a potential feed additive in aquaculture applications.

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Section: Introductionmentioning

confidence: 99%

The growth and probiotic characteristics of Bacillus velezensis BS in soybean meal used as synbiotic-like preparations for Litopenaeus vannamei culture

Hoang,

Nguyen,

Chu

et al. 2023

Vietnam J. Biotechnol.

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“…Application of microorganisms as biocontrol for reducing the pathogenic bacteria is an alternative method as substitution of antibiotic. Proteases can be used to improve feed quality by reducing the size of protein molecules to increase digestibility (Maytorena-Verdugo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

Trianto,

Radjasa,

Subagiyo

et al. 2023

Indo. J. Mar. Sci.

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Mangroves are complex, unique, and dynamic ecosystems that host organisms that have special physiological adaptations to fluctuations in salinity, temperature, and pH. Gastropods have an important role in the mangrove ecosystem for food cycles and webs by helping to provide nutrients and micro-habitats for microbes. Micro-fungi isolated from mangrove ecosystems are productive sources of enzymes and bioactive compounds such as antibacterial and antifungal. In this study a explored the enzymatic and anti-vibrio activity of fungi associated with the marine gastropod Cerithidea cingulata and Lottia scabra. collected in three mangrove forests in North Sulawesi, Indonesia. The fungi associated with the specimens were inoculated on malt extract agar (MEA) using the tapping method. Enzymatic and anti-vibrio assays were conducted applying the plug method. Positive effects of the fungal compounds on Vibrio were indicated by clear zones formed around the isolates. A total of 13 fungal species were isolated from C. cingulata (19MB-C5 and 19MBa-A4) and L. scabra (19MT-07 and 19MT-18). Most fungal isolates have moderate growth rate. The isolate with highest cellulolytic, proteolytic, and anti-vibriosis activity (Vibrio parahaemolyticus, V. harveyi, V. vulnificus, and V. alginolyticus), derived from C. cingulata 19MB-C5 was closely related to Aspergillus niger, as revealed by molecular analysis using the universal primers ITS1 and ITS4 (similarity value 99%). A. niger 19MB-C5-3 has potential as a bioremediation and anti-vibrio agent that can be applied in aquaculture.

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“…has the function to the inhibitor of trypsin and chymotrypsin, this highly stable proteinaceous inhibitor can resist the heat treatment during the drying process feed fabrication. Therefore the molecule is often present in its active form in commercial shrimp feed (Rojo-Arreola et al, 2019;Lemos et al, 2000 andMaytorena-Verdugo et al, 2017). Soybean Trypsin Inhibitor (S.B.T.I.)…”

Section: Introductionmentioning

confidence: 99%

“…Soybean Trypsin Inhibitor (S.B.T.I.) binds both trypsins and chymotrypsins of Litopenaeus vannamei with 80-90% inhibition at 0.25 mM (Rojo-Arreola et al, 2019;Hernandez-Cortes et al, 1997;Sainz et al, 2004;Maytorena-Verdugo et al, 2017). However, shrimp respond to phenotypic plasticity in the digestive system of L. vannamei as an adaptive response to compensate the protein digestion capacity when some of the peptidase activities are reduced by the presence of protease inhibitors (Rojo-Arreola et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

THE EVALUATION OF PROTEIN DIGESTIBILITY, SAPONIN AND TRYPSIN INHIBITOR CONTENT IN PACIFIC WHITE SHRIMP (Litopenaeus vanamei) FEED, DIGESTED WITH BROMELAIN CRUDE EXTRACT FROM PINEAPPLE WASTE

Klahan¹,

Bundit²,

Wiboonsirikul³

et al. 2022

Proceedings of International Conference on Fisheries and Aquaculture

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The present study aimed at investigating the effect of varying concentrations of bromelain crude extract from pineapple on percentage of protein digestibility and amount of saponin, and trypsin inhibitor in pacific white shrimp feed. The bromelain was extracted from the crown and peel of pineapple (Bhattavia strain) at dark green ripening stages. The trial was divided into two experiments; the first experiment determined the optimal condition for in vitro protein digestibility of pacific white shrimp feed which contains 38% of crude protein. It was digested by bromelain crude extract at different pH (6 -9), hydrolysis time (5, 10, and 30 min), and temperature (25 and 30 °C). The second experiment investigated the saponin and trypsin inhibitor (TI) in white shrimp feed after being digested with different concentrations of bromelain crude extract at 0, 90, 170, and 250 ppt for 5, 10, and 30 min at 30 °C. The results presented that the optimal condition for protein digestion with bromelain was pH 6 at 25 °C for 5 and 30 min, the percentage of protein digestion was 63.15 and 70.66% (P<0.05). Besides, saponin content in the diet was varied after the bromelain levels and hydrolysis time, the highest level of saponin was found in digested diet with bromelain at 170 and 250 ppt for 30 min (1.84 and 1.88 mg/g feed) and the lowest saponin at 90 and 170 ppt for 5 min. (0.94 and 0.99 mg/g feed) (P<0.05). The TI content in the diet was varied inversely with the levels of bromelain at 5 minute length which the lowest level of TI was shown in digested feed with bromelain at 250 ppt (0.008 mg/g feed) (P<0.05) whereas TI was varied with bromelain at 10 and 30minute lenght which the lowest level was shown at 0 ppt (0.0031 and 0.007mg/g feed) (P<0.05). This study showed that the optimal condition for protein digestion with bromelain was pH 6 at 25°C for 5 and 30 min, and the suitable level of bromelain and hydrolysis time to inactivate the saponin and trypsin inhibitor in shrimp feed were 250 ppt at 5 min.

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Peptidase compensation in the digestive system of whiteleg shrimp Penaeus vannamei against dietary Kunitz-type soybean trypsin inhibitor

Cited by 11 publications

References 45 publications

The growth and probiotic characteristics of Bacillus velezensis BS in soybean meal used as synbiotic-like preparations for Litopenaeus vannamei culture

The growth and probiotic characteristics of Bacillus velezensis BS in soybean meal used as synbiotic-like preparations for Litopenaeus vannamei culture

Molecular Identification and Biotechnological Potential of Cerithidea cingulata-and Lottia scabra Associated Fungi as Extracellular Enzyme Producer and Anti-Vibriosis Agent

THE EVALUATION OF PROTEIN DIGESTIBILITY, SAPONIN AND TRYPSIN INHIBITOR CONTENT IN PACIFIC WHITE SHRIMP (Litopenaeus vanamei) FEED, DIGESTED WITH BROMELAIN CRUDE EXTRACT FROM PINEAPPLE WASTE

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