Pepsin Secretion and Synthesis in vitro

Villarreal, Ramon

doi:10.3181/00379727-83-20502

Cited by 5 publications

(4 citation statements)

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“…A basal output of pepsin has previously been recorded from isolated stomach and gastric mucosa preparations of the rat (Mantovani, 1970), mouse (Villarreal, 1953) and rabbit (Kapadia & Donaldson, 1978) and a spontaneous output of pepsin was also observed from unstimulated stomachs in the present work. However, the control of this basal output is unclear since a high concentration of neither a histamine H2-receptor antagonist (cimetidine) nor a muscarinic blocker (atropine) had an effect on the secretion which was consistently of statistical significance.…”

Section: Effect Ofsecretory Antagonists On Histaminestimulated Secretionsupporting

confidence: 78%

“…However, the control of this basal output is unclear since a high concentration of neither a histamine H2-receptor antagonist (cimetidine) nor a muscarinic blocker (atropine) had an effect on the secretion which was consistently of statistical significance. Other workers have also reported that the basal secretion of pepsin from isolated tissue preparations is resistant to inhibition by both atropine and cimetidine (Villarreal, 1953;Kapadia & Donaldson, 1978). Histamine stimulated the secretion of pepsin in the rat isolated stomach, and such an effect of histamine has also been described in isolated tissue preparations of the mouse (Villarreal, 1953) and frog (Kasbekar & Gordon, 1979).…”

Section: Effect Ofsecretory Inhibitors On Basal Secretionmentioning

confidence: 99%

“…Other workers have also reported that the basal secretion of pepsin from isolated tissue preparations is resistant to inhibition by both atropine and cimetidine (Villarreal, 1953;Kapadia & Donaldson, 1978). Histamine stimulated the secretion of pepsin in the rat isolated stomach, and such an effect of histamine has also been described in isolated tissue preparations of the mouse (Villarreal, 1953) and frog (Kasbekar & Gordon, 1979). A direct comparison of pepsin output in these latter studies with the present work is made difficult by the use of different units to define peptic activity.…”

Section: Effect Ofsecretory Inhibitors On Basal Secretionmentioning

confidence: 99%

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The Effect of Cimetidine on Basal and Stimulated Pepsin Secretion in the Isolated Whole Stomach of the Rat

Bunce¹,

Grewal²,

Parsons³

1981

British J Pharmacology

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1 The isolated stomach preparation of the immature rat has been used to study the stimulation and inhibition of pepsin secretion. 2 The isolated stomach secretes a basal level of pepsin. High concentrations (10-3M) of the H2-receptor antagonist, cimetidine, and the muscarinic receptor blocking drug, atropine, did not affect this secretion in a manner which was consistently of statistical significance.3 Concentrations of histamine of 10-5M, 10-4M and 10-3M stimulated maximum levels of pepsin output of 126%, 155 % and 299% respectively of control. There was no evidence that this secretion was secondary to the stimulation of acid secretion. 4 Cimetidine (10-4M and 10-3M) produced a dose-related inhibition of the pepsin output to 10-3M histamine, suggesting that histamine H2-receptors mediate this response. 5 Atropine (10-3M) had no effect on the pepsin response to 10-3M histamine, suggesting that muscarinic mechanisms play no part, even modulatory, in this secretion.

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Section: Effect Ofsecretory Antagonists On Histaminestimulated Secretionsupporting

confidence: 78%

Section: Effect Ofsecretory Inhibitors On Basal Secretionmentioning

confidence: 99%

Section: Effect Ofsecretory Inhibitors On Basal Secretionmentioning

confidence: 99%

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The Effect of Cimetidine on Basal and Stimulated Pepsin Secretion in the Isolated Whole Stomach of the Rat

Bunce¹,

Grewal²,

Parsons³

1981

British J Pharmacology

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“…This animal preparation can secrete acid for long periods (Harper et al 1968) and the relatively high mucosal blood flow might deliver the precursor materials for pepsin secretion. However, the isolated mouse stomach under hyperbaric oxygen conditions secretes but does not synthesize pepsin (Villareal, 1953 Fig. 9.…”

Section: Pepsinogen Synthesis and Secretionmentioning

confidence: 99%

Pepsin secretion in the anaesthetized cat and the effect of sympathetic nerve stimulation.

Hooper¹,

Reed²,

Sanders³

1976

The Journal of Physiology

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SUMMARY1. Experiments were performed on chloralose anaesthetized cats and gastric mucosal blood flow, acid and pepsin secretions were measured. Gastric mucosal pepsin and protein contents were measured at the end of the experiments which were done in three groups: gastrin (A), vagal (B), vagal and sympathetic nerve (C) stimulations.2. Vagal stimulation significantly reduced (76 %) the pepsin content of gastric mucosa compared with gastrin stimulated animals, none of which secreted pepsin.3. The sum of the secreted and extracted pepsins for all the three groups was not significantly different. There was no significant difference in the extracted protein from any of the groups.4. Gastric mucosal blood flow, acid and pepsin outputs all had significant correlations with time during the first 70 min of vagal stimulation. During the period 80-160 min of vagal stimulation acid secretion and mucosal blood flow were not correlated with time but pepsin output declined significantly. From 170 to 220 min of vagal stimulation, acid and pepsin outputs and mucosal blood flow were not correlated with time.5. The assumed pepsin store during each period was calculated and after 40 min of vagal stimulation there is a constant percentage pepsin output from this assumed store.6. There is some data to suggest that pepsinogen synthesis was occurring during the period 170-220 min of vagal stimulation. 7. Sympathetic nerve stimulation which started at 160 min after the beginning of sustained vagal stimulation, significantly inhibited gastric acid secretion and mucosal blood flow, and in addition it significantly * Major Award, Hertfordshire County Education Committee.

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Some Reflections on Gastric Secretion

Davenport

1957

Gastroenterology

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Pepsin Secretion and Synthesis in vitro

Cited by 5 publications

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The Effect of Cimetidine on Basal and Stimulated Pepsin Secretion in the Isolated Whole Stomach of the Rat

The Effect of Cimetidine on Basal and Stimulated Pepsin Secretion in the Isolated Whole Stomach of the Rat

Pepsin secretion in the anaesthetized cat and the effect of sympathetic nerve stimulation.

Some Reflections on Gastric Secretion

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