Pentapeptide‐modified poly(N,N‐diethylacrylamide) hydrogel scaffolds for tissue engineering

Abstract

Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,N-diethylacrylamide (DEAAm), N,N'-methylenebisacrylamide and methacrylic acid in the presence of (NH₄)₂SO₄ or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH₂, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH₂ pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSR-NH₂-modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.

Human Embryonic Stem Cell-Derived Mesenchymal Stem Cell Seeding on Calcium Phosphate Cement-Chitosan-RGD Scaffold for Bone Repair

“…However, only a few studies have investigated hESCs for bone tissue engineering. 13,[23][24][25] There has been no report on hESC-derived MSC seeding on CPC. In a recent study, hESC-derived MSCs were encapsulated in alginate hydrogel microbeads and the microbeads were then incorporated into CPC; there was no direct cell attachment to the CPC surface.…”

“…13 Osteogenic differentiation of hESCs were achieved by introducing hESCs or hESCd-MSCs in the osteogenic medium 56,57 or cocultured with other cells. 58,59 Several types of scaffolds, such as collagen, 23 hydrogels, 25 polymer and composite, 13,24 were seeded with hESC-derived cells. However, few studies investigated the hESC seeding on biomaterials with high strength for loadbearing bone tissue engineering.…”

“…Recently, several studies investigated the use of hESCs for bone tissue engineering, and demonstrated that hESCs could undergo osteogenic differentiate in vitro and in vivo. 13,[23][24][25] Besides cells, scaffolds are another important component for tissue engineering. 26 Bioceramics, such as calcium phosphates are important for bone repair, because they can bond to bone to form a functional interface due to their compositions mimicking bone minerals to enhance cell attachment.…”

Thermoresponsive Nanogels Based on Different Polymeric Moieties for Biomedical Applications

“…Different research groups have studied PDEAAM’s properties, such as enhancement of thermosensitivity by copolymerization with 2-hydroxyethyl methacrylate (HEMA) [ 86 ] and thermal responsivity of PDEAAM hydrogels prepared by γ-ray irradiation [ 87 ]. In the past decade, PDEAAM has been used for the preparation of responsive hydrogels [ 88 , 89 , 90 ], micelles [ 91 , 92 ] and nanogels [ 93 , 94 ] for biomedical applications. Lu et al prepared non-ionic and thermosensitive nanogels with 100-nm diameters, based on N,N-diethyl acrylamide (DEA) and N,N-dimethylacrylamide (DMA), which can be used for DNA separation by microchip electrophoresis [ 95 ].…”

Non-Covalently Stabilized Alginate Hydrogels as Functional Cell Scaffold Material

“…Many approaches have been taken to design new hydrogel biomaterials for cell encapsulation ranging from synthetic structures, biological based structures, or chemically derivatives thereof . Biologically derived structures generally offer more potential to be clinically relevant as these are less likely to be cytotoxic, are often already known by regulatory bodies, and trigger only minimal immune responses .…”