Pentanoic acid induces thymic stromal lymphopoietin production through Gq/11 and Rho-associated protein kinase signaling pathway in keratinocytes

Mizuno, Natsumi; Abe, Koudai; Morishita, Yukari; Yamashita, Saori; Segawa, Ryosuke; Dong, Jing; Moriya, Takuya; Hiratsuka, Masahiro; Hirasawa, Noriyasu

doi:10.1016/j.intimp.2017.06.024

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…To further elucidate whether the mechanism of the neuroprotective effect of NOB is through inhibition of the Rho/ROCK signaling pathway, we established an OGD model of PC12 cells in vitro , examining the apoptosis, migration, cell viability and expression levels of related proteins after treatment of OGD PC12 cells with NOB, PA (an Rho/ROCK pathway agonist) ( 24 , 25 ) or NOB + PA. As shown in Figure 4 , the results revealed that the rate of apoptosis of PC12 cells in the NOB-treated group was less than that in the OGD model group. When treated with PA, apoptosis of OGD PC12 cells was accelerated, whereas treatment with NOB reduced PA-induced apoptosis.…”

Section: Resultsmentioning

confidence: 99%

Neuroprotective effects of nobiletin on cerebral ischemia/reperfusion injury rats by inhibiting Rho/ROCK signaling pathway

Huang¹,

Qin²,

Lü³

et al. 2022

Ann Transl Med

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Background: Nobiletin (NOB), an active natural flavonoid component of citrus, is used in Traditional Chinese Medicine for its anti-inflammatory activity, but its efficacy in cerebral ischemia/reperfusion (I/R) injury remains unclear. Methods: In a middle cerebral artery occlusion (MCAO) rat model, MCAO rats were administered (Sham group and MCAO model group treated with an equal volume of solvent, NOB group treated with 10 or 20 mg/kg NOB) once a day for 7 days before cerebral ischemia and again after reperfusion, 2,3,5-triphenyltetrazolium chloride (TTC) staining was applied to assess the infarct area. Neurological function was evaluated by the modified neurological severity score and Morris water maze. The levels of inflammatory factors, interleukin 6 (IL-6), interleukin 1β (IL-1β) and tumor necrosis factor-α (TNF-α), were examined by enzyme-linked immunosorbent assay (ELISA). Histopathological staining evaluated neuron apoptosis in brain tissue. In an oxygen-glucose deprivation PC12 cell (OGD PC12) model, the proliferation, migration and apoptosis of OGD PC12 cells were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and cell migration assays and flow cytometry. The gene and protein expression levels of Ras homolog gene family, member A (Rho A), ras-related C3 botulinum toxin substrate 1 (Rac 1), Rho-associated kinase 1 (ROCK 1), ROCK 2 in the Rho/ROCK pathway were measured by Realtime PCR (RT-PCR), immunohistochemistry and western blot.Results: In rats with cerebral I/R injury, NOB significantly decreased the infarcted area, neuron apoptosis in brain tissue and expressions of IL-6, IL-1β, and TNF-α. It also improved neurological deficits in brain tissue and enhanced learning and memory ability. Further, NOB had a protective effect on OGD PC12 cells, increasing proliferation and migration and decreasing apoptosis. The expressions of Rho A, Rac 1, ROCK 1 and ROCK 2 were high in cerebral I/R injury rats, but were downregulated by NOB in I/R injury rats' brain tissue and OGD PC12 cells.Conclusions: Nobiletin had a neuroprotective effect in rats with cerebral I/R injury, and its potential mechanism is decreasing neuron apoptosis by inhibiting the Rho/ROCK signaling pathway. These results suggest NOB is a promising neuroprotective agent for patients with cerebral ischemia.

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Section: Resultsmentioning

confidence: 99%

Neuroprotective effects of nobiletin on cerebral ischemia/reperfusion injury rats by inhibiting Rho/ROCK signaling pathway

Huang¹,

Qin²,

Lü³

et al. 2022

Ann Transl Med

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“…CIC formation between tumor cells has been reported to alter energy metabolism, which closely related to the occurrence and development of tumors [ 31 , 32 ]. We treated the U2OS cells with MT, MT + ROCK activator (pentanoic acid) [ 39 ], or MT + ROCK inhibitor (ROCK inhibitor-2) [ 40 ], and determined the relative fluorescence of mitochondria by immunofluorescence. We observed that MT and MT + ROCK inhibitor reduced the relative fluorescence of mitochondria in OS cells (P < 0.05, Fig.…”

Section: Resultsmentioning

confidence: 99%

3D-printing magnesium–polycaprolactone loaded with melatonin inhibits the development of osteosarcoma by regulating cell-in-cell structures

et al. 2021

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Melatonin has been proposed as a potent anticarcinogen presents a short half-life for osteosarcoma (OS). Cell-in-cell (CIC) structures play a role in the development of malignant tumors by changing the tumor cell energy metabolism. This study developed a melatonin-loaded 3D printed magnesium–polycaprolactone (Mg–PCL) scaffold and investigated its effect and molecular mechanism on CIC in OS. Mg–PCL scaffold was prepared by 3D-printing and its characteristic was determined. The effect and molecular mechanism of Mg–PCL scaffold as well as melatonin-loaded Mg–PCL on OS growth and progression were investigated in vivo and in vitro. We found that melatonin receptor 1 (MT1) and CIC expressions were increased in OS tissues and cells. Melatonin treatment inhibit the key CIC pathway, Rho/ROCK, through the cAMP/PKA signaling pathway, interfering with the mitochondrial physiology of OS cells, and thus playing an anti-invasion and anti-metastasis role in OS. The Mg–PCL–MT could significantly inhibit distant organ metastasis of OS in the in vivo model. Our results showed that melatonin-loaded Mg–PCL scaffolds inhibited the proliferation, invasion and metastasis of OS cells through the CIC pathway. The Mg–PCL–MT could be a potential therapeutics for OS.

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“…SCFAs are also known to mediate inflammatory response through these GPCRs, but allergic response elicited by pentanoic acid, in keratinocytes appears to be dependent on a Gq/11-coupled protein receptor that is not GPR41 or GPR43. This suggests that SCFAs may have yet more unidentified receptors within the body [ 57 ]. The differential release of SCFAs for LMW-HDE and HMW-LDE lemon pectin may promote cellular health in slightly different, but complementary ways.…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Gut Microbiota Structure and Function by Two Structurally Different Lemon Pectins

Firrman

Mahalak

Bobokalonov

et al. 2022

Foods

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Pectins are plant polysaccharides consumed as part of a diet containing fruits and vegetables. Inside the gastrointestinal tract, pectin cannot be metabolized by the mammalian cells but is fermented by the gut microbiota in the colon with the subsequent release of end products including short-chain fatty acids (SCFA). The prebiotic effects of pectin have been previously evaluated but reports are inconsistent, most likely due to differences in the pectin chemical structure which can vary by molecular weight (MW) and degree of esterification (DE). Here, the effects of two different MW lemon pectins with varying DEs on the gut microbiota of two donors were evaluated in vitro. The results demonstrated that low MW, high DE lemon pectin (LMW-HDE) altered community structure in a donor-dependent manner, whereas high MW, low DE lemon pectin (HMW-LDE) increased taxa within Lachnospiraceae in both donors. LMW-HDE and HMW-LDE lemon pectins both increased total SCFAs (1.49- and 1.46-fold, respectively) and increased acetic acid by 1.64-fold. Additionally, LMW-HDE lemon pectin led to an average 1.41-fold increase in butanoic acid. Together, these data provide valuable information linking chemical structure of pectin to its effect on the gut microbiota structure and function, which is important to understanding its prebiotic potential.

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Pentanoic acid induces thymic stromal lymphopoietin production through Gq/11 and Rho-associated protein kinase signaling pathway in keratinocytes

Cited by 10 publications

References 32 publications

Neuroprotective effects of nobiletin on cerebral ischemia/reperfusion injury rats by inhibiting Rho/ROCK signaling pathway

Neuroprotective effects of nobiletin on cerebral ischemia/reperfusion injury rats by inhibiting Rho/ROCK signaling pathway

3D-printing magnesium–polycaprolactone loaded with melatonin inhibits the development of osteosarcoma by regulating cell-in-cell structures

Modulation of the Gut Microbiota Structure and Function by Two Structurally Different Lemon Pectins

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