PENGEMBANGAN DIAGNOSIS TUBERKULOSIS PADA HEWAN KESAYANGAN ANJING MENGGUNAKAN ANTIGEN SPESIFIK Mycobacterium tuberculosisESAT-6 DAN CFP-10

Abstract

Early diagnosis is one of the important methods to control tuberculosis because this disease is zoonotic which easily spread through the air. Early detection of tuberculosis in dog is also very important since dog as pet animal have a potency to transfer the disease to human or other animals. The discovery of two specific M.tuberculosis antigens, ESAT06 and CFP-10, provide the opportunity to develop a specific diagnostic kit for tuberculosis by using ELISA based on the secretion of IFN-γ. The development of a tuberculosis diagnostic kit based on this molecular biology and immunological method would provide a good alternative method to detect tuberculosis specially, accurately as early as possible. The result of this experiment would provide contribution for the development of health science and technology, especially in the eradication of tuberculosis.

VAKSINASI BCG MENINGKATKAN AKTIVITAS FAGOSITOSIS DAN SEKRESI REACTIVE OXYGEN INTERMEDIATE (ROI) PADA MAKROFAG PERITONEUM KUCING YANG DIINFEKSI DENGAN Mycobacterium tuberculosis

“…Laporan lain mengenai efek BCG terhadap pembunuhan Plasmodium berghei oleh makrofag didemontrasikan oleh Wijayanti (15). Hasil penelitian terdahulu menunjukkan bahwa infeksi tuberkulosis pada kucing juga mengikuti paradigma Th1 dan Th2 yang melibatkan aktivitas makrofag dan T helper (16). Meskipun BCG sampai saat ini digunakan di lapangan dan telah diketahui bersifat imunopotensiator, namun bagaimana pengaruhnya terhadap imunitas seluler khususnya aktivitas makrofag dalam fagositosis dan sekresi ROI pada kucing belum pernah dilaporkan.…”