Pengaruh Waktu Penyimpanan Darah Terhadap Kadar Hemoglobin Pada Komponen Whole Blood Darah Donor

Abstract

Blood transfusion is the process of distributing blood from donors to patients with the aim of replacing blood lost due to bleeding, surgery, shock and malfunctioning of the blood-forming organs that require replacement blood in the form of whole blood or blood components. Blood is stored with the First in First Out (FIFO) system, which is a system that regulates the expulsion of blood in which the first blood that enters will be removed first. The storage period of blood will experience changes in blood components, especially erythrocytes will experience significant changes in shape over the length of time blood storage. The purpose of this study was to determine the difference in hemoglobin levels of donor blood before and after storage for 7 days. This research was conducted at the Blood Transfusion Unit of PMI Kudus Regency. The number of samples used as many as 15 samples. The results showed that there were differences in hemoglobin levels before storage and after storage for 7 days (0.000). The average value before storage was 14.7 g/dl, after storage for 7 days 18.2 g/dl, the highest hemoglobin before storage was 15.4 g/dl, the highest after storage was 18.2 g/dl, while the lowest hemoglobin was before storage. storage 14.0 gr/dl and after storage 17.3 gr/dl. The conclusion is there is a significant difference between hemoglobin levels before storage and after storage for 7 days.

Erythrogram Profile of Blood Samples Anticoagulated with Tri-potassium Ethylene Diamine Tetraacetic Acid (K3EDTA) Stored for 48 Hours at 4oC

“…The dynamics of hemoglobin levels during the storage period could be influenced by erythrocyte damage. Saputro and Lestari (2021) reported that stored blood samples could increase hemoglobin levels. The longer the stored blood sample, the more erythrocytes can swell due to stiffness and loss of lipids in the cell membrane.…”

“…This study found hypochromasia in blood samples stored for 6 to 48 hours, with a score of 4+. Saputro and Lestari (2021) reported that the longer blood samples are stored, the more hemoglobin in the erythrocytes will be released into the blood plasma, so that the hemoglobin level in the erythrocytes decreases and can cause hypochromasia in the erythrocytes.…”

“…Many factors influence the erythrocyte morphology, including the K3EDTA anticoagulant, length of storage period, and temperature during storage (Fitria et al, 2016;Rahmanitarini et al, 2019;Saputro and Lestari, 2021). During storage, erythrocytes undergo several changes that affect their ability to carry oxygen.…”

Temperature Distribution Monitoring on Blood Bank Chamber Using Android Application on Mobile Phone

“…Meanwhile, storage at temperatures above 6°C causes the growth of bacteria very quickly so that blood transfusions can be fatal for the recipient of the blood [3]. The temperature limit of 2°C is also very important because red blood cells are very sensitive to clotting, where if the red blood cells freeze, the blood cell walls will burst and the hemoglobin will come out (hemolysis) [4] [5]. Therefore, blood banks need to ensure the quality of blood so that there is no risk of damage during storage.…”