PENGARUH PENAMBAHAN FILTRAT ROSELLA (Hibiscus sabdariffa Linn) KE DALAM PENGENCER TRIS-KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING KACANG (The effect of rosella filtrate suplementation in tris-egg yolk extender on sperm.....

Abstract

The purpose of this study was to determinate the effect of adding various levels of roselle filtrate (RF) into tris-egg yolk diluents (T-EY) on the quality of kacang goat spermatozoa. Semen was collected twice a week using an artificial vagina method of three male goats. Fresh semen obtained was evaluated macroscopically and microscopically with a motility value of 80%, viability 86.58%, concentration of 2,099.40 milionspermatozoa cells/ml, and an abnormality of 2.83%. Then the fresh semen was diluted according to the treatment, namely T0 (T-EY 100%), T1 (T-EY 100% + RF 1%), T2 (T-EY 100% + RF 2%), T3 (T-EY 100% + RF 3%), T4 (T-EY 100% + RF 4%), T5 (T-EY 100% + RF 5%). The diluted semen was stored at 3-5C, and evaluation was carried out every 24 hours on the quality of the spermatozoa. The results showed that spermatozoa in T-EY diluent with the addition of 3% RF (T3) had a higher quality (T<0.05) compered to the other five treatments, namely the motility reached (42,99±2,19%), viability (49,30±2,24%), and abnormalities (4,012±0,49%) during 5 days of storage. The conclusion of this study is the addition of 3% roselle filtrate in tris-egg yolk diluent is the best level and is more effective in maintaining the quality of spermatozoa kacang goat.

Characteristics and Kinematics of Bali Bull Sperms after Thawing Using Tris Soy Lecithin

“…These results indicate that the semen volume of Bali bull in this study was lower compared to the findings of previous study byWijayanti et al, (2023), which recorded a volume of 6.28 ml. However, this semen volume is almost close to reported byMujahidurrohman et al, (2023), which was 4.92 ml and was higher than the findings reported byLeo et al, (2023) of 3.20 ml. Differences in semen volume during shelter can be influenced by several factors, such as differences in body size, a Characteristics and Kinematics of Bali Bull Sperms after Thawing Using Tris Soy Lecithin Available online at: https://jazindia.com -482 -offspring…”

Addition of Sugarcane Juice to Tris Egg Yolk Buffer Diluent Improves the Quality of the Stored Diluted Semen of Kacang Goats at 5°C

“…In this study, the viability percentage obtained after 4 days of storage was slightly better than the result of, which used 10% pomegranate juice, which was 74.8 with a viability value of 56.85% , 66.0±7.9 when extender was enriched with 7% fig fruit filtrate (Zaenuri et al, 2013). This difference was caused by the physical and chemical properties and levels of diluent in the treatment, osmotic pressure, electrolytes, and non-electrolytes (Laos et al, 2021). Membrane structure eventually reduces its viability when liquid semen is stored at 5°C due to oxidative stress, triggering the formation of reactive oxygen (Zaenuri et al, 2014).…”

“…The high viability of all treatments with the addition of the sugarcane juice extract was because the nutrient in the sugarcane juice effectively maintained the viability of the spermatozoa of the Kacang goat. Laos et al (2021) stated that spermatozoa utilized sucrose from sugarcane extract in metabolism to support the energy needed for movement. The average percentage of spermatozoa viability after 4 days of storage had the best results at T2 treatment, with a value of 79.4±1.14%.…”

“…Moreover, the results showed that semen stored longer at both room temperature and 5°C experienced a decrease in the percentage of viability. This was because changes in the structure of the phospholipids plasma membrane occurred during storage, which disrupted the function and permeability of the cell plasma membrane (Laos et al, 2021). When damage to the cell plasma membrane occurred, it interfered with metabolic processes, and the synthesis process of ATP was inhibited, causing decreased viability of spermatozoa (Sukmawati et al, 2015).…”

Penambahan Filtrat Kelopak Rosella (Hibiscus Sabdariffa) dalam Pengencer Tris Kuning Telur pada Semen Beku Kambing Kaligesing

“…Vitamin C berfungsi sebagai antioksidan serta dapat meningkatkan laju fruktolisis. Peningkatan fruktolisis dapat memenuhi kebutuhan energi spermatozoa (Laos, 2021). Semakin tinggi kandungan vitamin C maka akan semakin kuat dalam menangani radikal bebas (Lestiana et al, 2019).…”