ABSTRAKAngka kebuntingan sapi bali yang mengalami sinkronisasi estrus dan diinseminasi pada saat estrus muncul masih di bawah 50%. Akan tetapi, persentrasi gejala estrus yang muncul setelah sinkronisasi selalu di atas 85%. Oleh karena itu, penelitian untuk mengevaluasi tingkat kebuntingan sapi bali setelah sinkronisasi, deteksi estrus dan perkawinan baik kawin alam maupun inseminasi telah dilakukan dengan menggunakan 67 ekor sapi bali induk umur 4-6 tahun yang mempunyai corpus luteum periodik. Penelitian dilakukan sebanyak empat tahap dengan masing-masing tahap selama 22 hari. Semua sapi percobaan dipelihara secara intensif pada 5 pedok yang terpisah dan setiap pedok ditempatkan 4 ekor sapi bali induk dan seekor pejantan. Hormon estron digunakan untuk sinkronisasi induk sapi percobaan dengan dosis 5 ml/ekor secara intra muscular, selanjutnya dilakukan induk sapi dikawinkan pada hari pertama sampai kelima setelah sinkronisasi dan deteksi estrus. Deteksi estrus diperpanjang sampai dengan akhir siklus estrus. Hasil penelitian menunjukkan bahwa 91,05% sapi percobaan mengalami estrus setelah sinkronisasi sedangkan gejala estrus muncul mulai hari pertama sampai hari kelima (rata-rata hari ke 3) setelah sinkronisasi. Angka kebuntingan yang diperoleh rata-rata 27,87%. Estrus alami (terjadi setelah estrus sinkronisasi) mulai muncul setelah 6-13 hari (rata-rata 8 hari) estrus hasil sinkronisasi muncul.Kata Kunci: estrus, kebuntingan, PGF2α Therefore, the experiment related to evaluation of conception rate of bali cow following synchronization, estrus detection and natural mating or artificial insemination was conducted using 67 bali cow aged 4-6 y.o. that has periodic corpus luteum. The experiment was conducted in four sequent periods (22 days each). All animal was kept intensively in 5 separate paddocks where each paddock was allocated five animals (4 cows+1 bull). Estron contained PGF2α was injected intramuscular (5 ml/head) to all cows for synchronization while mating (natural mating and artificial insemination) was conducted during 1-5 days following synchronization and estrus detection. Estrus detection was prolonged up to the end of estrus cycle. The results showed that 91.05% of synchronized cow was estrus, while the estrus sign occurred on day 1-5 (day 3 in average) after synchronization. Conception rate gained in this experiment was 27.87%. Natural estrus that occurred following synchronized estrus varied between 6-13 days (8 days in average) after synchronized estrus occurred.Keywords: conception, estrus, PGF2α, synchronization
This study determined whether the administration of progesterone and hCG after insemination increased pregnancy rate in Bali Timor cows. A total of 30 Bali Timor cows (BW ± 200-225 kg; aged 3-7 years old) with different parity were used in this study. Cows were inseminated with progesterone and hCG. Cows were grouped based on body weight and number of parities following a Complete Randomized Design with 3 treatments and 10 replications. The treatments were: P1 = did not receive any treatment (control); P2 = estrus induced by PGF2α 125 mg on day 5, 7 and 9 (n = 10); and P3 = estrus induced by hCG 1000 IU on day 5 after artificial insemination (AI). Blood samples were collected from the jugular vein of the animals on day 0 and continued for a period of 33 days after AI using 5 mL heparinised pre-set syringes Blood samples were then centrifuged at 1500 rpm for 10 minutes. Serum was harvested and stored at -20oC until serum concentrations of progesterone and hCG were determined using ELISA procedures. Pregnancy was determined by rectal palpation. The results showed that the AI pregnancy rates was greater in group treated with hCG (70%) than cows induced with progesterone (50%) or the control group. The concentrations of both progesterone and hCG were increased simultaneously on day 5 to 11 post AI. In conclusion, progesterone and hCG application in Bali Timor cows after artificial insemination could increase pregnancy rates.
The quality of porcine semen deteriorates very rapidly during the semen preservation process. The aim of this study is to find alternative diluents for Duroc semen preservation by changing different semen diluents. Two Duroc pigs, ± 3 years old, were trained twice weekly for semen collection. Immediately after collection, sperm were examined macroscopically and microscopically. Semen with sperm motility ≥ 70%, sperm concentration ≥200 x 106 cells/ml, and abnormality ≤20%) was diluted with six diluents, namely: Beltsville Thawing Solution® (BTS), Durasperm, Citrate Egg Yolk (CEY), Tris Egg Yolk (TEY), CEY + Olive Oil (CEYO) and TEY + Olive Oil (TEYO) in the ratio of one part semen and four parts diluent. The liquid sperm was stored at 18-20 oC and examined every eight hours for 80 hours. The result shows that sperm motility, viability and plasma membrane integrity of Duroc semen with CEYO and TEYO are comparable to BTS. No significant difference was found in sperm abnormalities. The results suggest that citrate egg yolk and Tris egg yolk in combination with olive oil are suitable as alternative diluents for the preservation of porcine sperm. Further studies are needed to determine the most suitable olive oil concentration for the preservation of porcine sperm of different breeds.
The aims of this research were to estimate repitability coefficients of production at birth and weaning characters and to determine the average and standard deviation of landrace crossbreed sows. This research was carried out in two breeding farms namely the Instalation of Pig Breeding, village of Tarus, Kupang Regency and the Manise Pig Farm, Village of Oetete, Kupang City, East Nusa Tenggara. Materials used 15heads landrace crossbreed sows ( 15 heads ) which born and weaned piglets during the study periode. The number of piglets for the first parity was 151heads and for the second parity was 162heads. All sows was mated by artivicial insemination with boars of landrace crossbreed. Variables were liter size, weaning rate, birth weight, weaning weight and weaning rate. Results showed that landrace crossbreed sows had the average and deviation standard for the first and second liter size were 10.00±1.50heads and 10.73±2.40heads, respectively; for the first and second birth weight were 1.37±0.23kg and 1.68±0.34kg, respectively; for the first and second weaning weight were 6.42±0.38kg and 6.72±0.51kg, respectively and for the first and second weaning rate were 8.33±1.28heads and 8.68±1.43 heads; respectively. The average of litter size, birth weight, weaning weight and weaning rate between the first and second parity was no difference (P > 0,05). The value of repeatabitily for characters of liter size, birth weight, weaning weight and weaning rate were 0.32; 0.47; 0.49 and 0,42; respectively. In conclusion, the repeatabily value of litter size, birth weight, weaning weight and weaning rate from landrace crossbred sows were medium value and the performance of those characters between the first and second parity were no difference.
This aim of the research was to know the influence of sex on production performances at birth age of piglets of duroc sow crossbreds. The research was carried out in two breeding farms namely the Instalation of Pig Breeding, village of Tarus, Kupang Regency and the Manise Pig Farm, Village of Oetete, Kupang City, East Nusa Tenggara (NTT). There were 20 heads of duroc crossbred sows. The method used a survey and samples were collected purposively. Samples were all pigles that born during research period from all of duroc sow crossbreds. The variables were litter size, body weight, girth size, and body length. Data was differentiated between males and females and analysed using the t test. The results showed that males had the average of litter size, body weight, girth size and body length of 4.94±1.21 head; 1.42±0.55kg; 23.94±2.80cm and 21.91±4.06cm, respectively; while females had the average of liter size, body weight, girth size and body length of 4.60±1.32 head; 1.39±0.38kg; 23.20±2.76cm and 20.93±4.20cm, respectively. Statistical analysis showed that there was no difference (P>0.05) between males and females from observation variables. Performances of piglets from duroc sow crossbreds for male and female characters such as litter size, birth weight, girth size and body length were relatively similar.
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